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miR-965 controls cell proliferation and migration during tissue morphogenesis in the Drosophila abdomen.

Verma P, Cohen SM - Elife (2015)

Bottom Line: During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis.Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis.By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular and Cell Biology, Singapore, Singapore.

ABSTRACT
Formation of the Drosophila adult abdomen involves a process of tissue replacement in which larval epidermal cells are replaced by adult cells. The progenitors of the adult epidermis are specified during embryogenesis and, unlike the imaginal discs that make up the thoracic and head segments, they remain quiescent during larval development. During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis. Here, we provide evidence that the microRNA, miR-965, acts via string and wingless to control histoblast proliferation and migration. Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis. Replacement of the larval epidermis by adult epidermal progenitors involves regulation of both cell-intrinsic events and cell communication. By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

No MeSH data available.


Related in: MedlinePlus

Rescue of the migration defect of miR-965 mutants with reduced levels of string.Left: still images taken from a time-lapse video showing histoblast divisions in the miR-965 mutant (KO1/KO2) with reduced levels of string transcript using the stgEY12388 allele. M1 and M2 indicate mitosis 1 and 2. Cell membranes were labeled using Atpα-GFP (green). Nuclei were labeled using Histone2-RFP (red). ADHN: anterior dorsal histoblast nest. PDHN: posterior dorsal histoblast nest. Scale bars: 50 µM. Right: still images taken from a time-lapse video showing histoblast nest migration in animals of the same genotypes. Scale bars: 100 µM. Refers to Figure 5D and Video 11.DOI:http://dx.doi.org/10.7554/eLife.07389.025
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fig5s4: Rescue of the migration defect of miR-965 mutants with reduced levels of string.Left: still images taken from a time-lapse video showing histoblast divisions in the miR-965 mutant (KO1/KO2) with reduced levels of string transcript using the stgEY12388 allele. M1 and M2 indicate mitosis 1 and 2. Cell membranes were labeled using Atpα-GFP (green). Nuclei were labeled using Histone2-RFP (red). ADHN: anterior dorsal histoblast nest. PDHN: posterior dorsal histoblast nest. Scale bars: 50 µM. Right: still images taken from a time-lapse video showing histoblast nest migration in animals of the same genotypes. Scale bars: 100 µM. Refers to Figure 5D and Video 11.DOI:http://dx.doi.org/10.7554/eLife.07389.025


miR-965 controls cell proliferation and migration during tissue morphogenesis in the Drosophila abdomen.

Verma P, Cohen SM - Elife (2015)

Rescue of the migration defect of miR-965 mutants with reduced levels of string.Left: still images taken from a time-lapse video showing histoblast divisions in the miR-965 mutant (KO1/KO2) with reduced levels of string transcript using the stgEY12388 allele. M1 and M2 indicate mitosis 1 and 2. Cell membranes were labeled using Atpα-GFP (green). Nuclei were labeled using Histone2-RFP (red). ADHN: anterior dorsal histoblast nest. PDHN: posterior dorsal histoblast nest. Scale bars: 50 µM. Right: still images taken from a time-lapse video showing histoblast nest migration in animals of the same genotypes. Scale bars: 100 µM. Refers to Figure 5D and Video 11.DOI:http://dx.doi.org/10.7554/eLife.07389.025
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4538364&req=5

fig5s4: Rescue of the migration defect of miR-965 mutants with reduced levels of string.Left: still images taken from a time-lapse video showing histoblast divisions in the miR-965 mutant (KO1/KO2) with reduced levels of string transcript using the stgEY12388 allele. M1 and M2 indicate mitosis 1 and 2. Cell membranes were labeled using Atpα-GFP (green). Nuclei were labeled using Histone2-RFP (red). ADHN: anterior dorsal histoblast nest. PDHN: posterior dorsal histoblast nest. Scale bars: 50 µM. Right: still images taken from a time-lapse video showing histoblast nest migration in animals of the same genotypes. Scale bars: 100 µM. Refers to Figure 5D and Video 11.DOI:http://dx.doi.org/10.7554/eLife.07389.025
Bottom Line: During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis.Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis.By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular and Cell Biology, Singapore, Singapore.

ABSTRACT
Formation of the Drosophila adult abdomen involves a process of tissue replacement in which larval epidermal cells are replaced by adult cells. The progenitors of the adult epidermis are specified during embryogenesis and, unlike the imaginal discs that make up the thoracic and head segments, they remain quiescent during larval development. During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis. Here, we provide evidence that the microRNA, miR-965, acts via string and wingless to control histoblast proliferation and migration. Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis. Replacement of the larval epidermis by adult epidermal progenitors involves regulation of both cell-intrinsic events and cell communication. By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

No MeSH data available.


Related in: MedlinePlus