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miR-965 controls cell proliferation and migration during tissue morphogenesis in the Drosophila abdomen.

Verma P, Cohen SM - Elife (2015)

Bottom Line: During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis.Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis.By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular and Cell Biology, Singapore, Singapore.

ABSTRACT
Formation of the Drosophila adult abdomen involves a process of tissue replacement in which larval epidermal cells are replaced by adult cells. The progenitors of the adult epidermis are specified during embryogenesis and, unlike the imaginal discs that make up the thoracic and head segments, they remain quiescent during larval development. During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis. Here, we provide evidence that the microRNA, miR-965, acts via string and wingless to control histoblast proliferation and migration. Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis. Replacement of the larval epidermis by adult epidermal progenitors involves regulation of both cell-intrinsic events and cell communication. By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

No MeSH data available.


Related in: MedlinePlus

Pupal survival assays.Pupal survival was assayed for flies of the indicated genotypes. 6 batches of pupae were sampled/genotype. The data present the total number of surviving adults (live) and the total number of dead pupae (dead). There was no significant difference between the mutant and control genotypes used to make the videos: p = 0.67 comparing KO2 esgG4>GFP/+ vs KO2 esgG4>GFP/KO1 (Mann–Whitney test). p = 1 comparing KO1 esgG4>GFP/+ vs KO1 esgG4>GFP/KO2 (Mann–Whitney test). Refers to Figure 3B.DOI:http://dx.doi.org/10.7554/eLife.07389.011
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fig3s3: Pupal survival assays.Pupal survival was assayed for flies of the indicated genotypes. 6 batches of pupae were sampled/genotype. The data present the total number of surviving adults (live) and the total number of dead pupae (dead). There was no significant difference between the mutant and control genotypes used to make the videos: p = 0.67 comparing KO2 esgG4>GFP/+ vs KO2 esgG4>GFP/KO1 (Mann–Whitney test). p = 1 comparing KO1 esgG4>GFP/+ vs KO1 esgG4>GFP/KO2 (Mann–Whitney test). Refers to Figure 3B.DOI:http://dx.doi.org/10.7554/eLife.07389.011

Mentions: It was noted that the migration defects observed in the videos seem to be more severe than the segmentation defects observed in the adult flies. However, we did not observe significant pre-eclosion lethality (Figure 3—figure supplement 3). Given that there was no evidence for loss of a class of more severely affected animals, we suggest that the apparent difference reflects a delay in tissue replacement in the mutant, so that most animals end up with milder defects by the end of pupariation than were apparent during the early pupal time-window in the videos.


miR-965 controls cell proliferation and migration during tissue morphogenesis in the Drosophila abdomen.

Verma P, Cohen SM - Elife (2015)

Pupal survival assays.Pupal survival was assayed for flies of the indicated genotypes. 6 batches of pupae were sampled/genotype. The data present the total number of surviving adults (live) and the total number of dead pupae (dead). There was no significant difference between the mutant and control genotypes used to make the videos: p = 0.67 comparing KO2 esgG4>GFP/+ vs KO2 esgG4>GFP/KO1 (Mann–Whitney test). p = 1 comparing KO1 esgG4>GFP/+ vs KO1 esgG4>GFP/KO2 (Mann–Whitney test). Refers to Figure 3B.DOI:http://dx.doi.org/10.7554/eLife.07389.011
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4538364&req=5

fig3s3: Pupal survival assays.Pupal survival was assayed for flies of the indicated genotypes. 6 batches of pupae were sampled/genotype. The data present the total number of surviving adults (live) and the total number of dead pupae (dead). There was no significant difference between the mutant and control genotypes used to make the videos: p = 0.67 comparing KO2 esgG4>GFP/+ vs KO2 esgG4>GFP/KO1 (Mann–Whitney test). p = 1 comparing KO1 esgG4>GFP/+ vs KO1 esgG4>GFP/KO2 (Mann–Whitney test). Refers to Figure 3B.DOI:http://dx.doi.org/10.7554/eLife.07389.011
Mentions: It was noted that the migration defects observed in the videos seem to be more severe than the segmentation defects observed in the adult flies. However, we did not observe significant pre-eclosion lethality (Figure 3—figure supplement 3). Given that there was no evidence for loss of a class of more severely affected animals, we suggest that the apparent difference reflects a delay in tissue replacement in the mutant, so that most animals end up with milder defects by the end of pupariation than were apparent during the early pupal time-window in the videos.

Bottom Line: During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis.Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis.By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular and Cell Biology, Singapore, Singapore.

ABSTRACT
Formation of the Drosophila adult abdomen involves a process of tissue replacement in which larval epidermal cells are replaced by adult cells. The progenitors of the adult epidermis are specified during embryogenesis and, unlike the imaginal discs that make up the thoracic and head segments, they remain quiescent during larval development. During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis. Here, we provide evidence that the microRNA, miR-965, acts via string and wingless to control histoblast proliferation and migration. Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis. Replacement of the larval epidermis by adult epidermal progenitors involves regulation of both cell-intrinsic events and cell communication. By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

No MeSH data available.


Related in: MedlinePlus