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miR-965 controls cell proliferation and migration during tissue morphogenesis in the Drosophila abdomen.

Verma P, Cohen SM - Elife (2015)

Bottom Line: During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis.Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis.By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular and Cell Biology, Singapore, Singapore.

ABSTRACT
Formation of the Drosophila adult abdomen involves a process of tissue replacement in which larval epidermal cells are replaced by adult cells. The progenitors of the adult epidermis are specified during embryogenesis and, unlike the imaginal discs that make up the thoracic and head segments, they remain quiescent during larval development. During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis. Here, we provide evidence that the microRNA, miR-965, acts via string and wingless to control histoblast proliferation and migration. Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis. Replacement of the larval epidermis by adult epidermal progenitors involves regulation of both cell-intrinsic events and cell communication. By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

No MeSH data available.


Related in: MedlinePlus

Rate of histoblast nest expansion measured from time-lapse videos.Each data point corresponds to one histoblast nest. The leading edge of each histoblast nest was tracked using imageJ. Speed was calculated measuring total distance covered (micrometer/hour). Genotypes: Control was esg-GAL4, UAS-GFP. esg-GAL4 and UAS-GFP were recombined onto the KO1 and KO2 chromosomes and onto the miR-965-Rescue chromosome. Data include examples with both recombinant mutant chromosomes. No difference between these two recombinants was apparent. n = 18 for the miR-965 (KO1/KO2) mutant combination. n = 15 for control and rescue. Left panel: p < 0.0001 comparing KO1/KO2 with control, p < 0.01 comparing KO1/KO2 with rescue using one-way ANOVA. Right panel: p < 0.001 comparing KO1/KO2 with control, p < 0.01 comparing KO1/KO2 with rescue using one-way ANOVA. Refers to Figure 3B and Videos 5–7.DOI:http://dx.doi.org/10.7554/eLife.07389.009
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fig3s1: Rate of histoblast nest expansion measured from time-lapse videos.Each data point corresponds to one histoblast nest. The leading edge of each histoblast nest was tracked using imageJ. Speed was calculated measuring total distance covered (micrometer/hour). Genotypes: Control was esg-GAL4, UAS-GFP. esg-GAL4 and UAS-GFP were recombined onto the KO1 and KO2 chromosomes and onto the miR-965-Rescue chromosome. Data include examples with both recombinant mutant chromosomes. No difference between these two recombinants was apparent. n = 18 for the miR-965 (KO1/KO2) mutant combination. n = 15 for control and rescue. Left panel: p < 0.0001 comparing KO1/KO2 with control, p < 0.01 comparing KO1/KO2 with rescue using one-way ANOVA. Right panel: p < 0.001 comparing KO1/KO2 with control, p < 0.01 comparing KO1/KO2 with rescue using one-way ANOVA. Refers to Figure 3B and Videos 5–7.DOI:http://dx.doi.org/10.7554/eLife.07389.009

Mentions: Subsequently, the rate of histoblast nest expansion was slower in the miR-965 mutant, compared to the controls (Figure 3B). Expansion of the histoblast nests was quantified in segments 3 and 4 by monitoring the speed of migration (Figure 3—figure supplement 1, Videos 5, 6). The average speed of migration of third and fourth histoblast nests in the control samples was 15 µm/hr, compare with ∼6.5 µm/hr in the mutant. The rate of nest expansion was increased to 12–14 µm/hr by restoring miR-965 expression with the rescue allele the miR-965 mutant background (Video 7).Video 5.Control, growth phase.


miR-965 controls cell proliferation and migration during tissue morphogenesis in the Drosophila abdomen.

Verma P, Cohen SM - Elife (2015)

Rate of histoblast nest expansion measured from time-lapse videos.Each data point corresponds to one histoblast nest. The leading edge of each histoblast nest was tracked using imageJ. Speed was calculated measuring total distance covered (micrometer/hour). Genotypes: Control was esg-GAL4, UAS-GFP. esg-GAL4 and UAS-GFP were recombined onto the KO1 and KO2 chromosomes and onto the miR-965-Rescue chromosome. Data include examples with both recombinant mutant chromosomes. No difference between these two recombinants was apparent. n = 18 for the miR-965 (KO1/KO2) mutant combination. n = 15 for control and rescue. Left panel: p < 0.0001 comparing KO1/KO2 with control, p < 0.01 comparing KO1/KO2 with rescue using one-way ANOVA. Right panel: p < 0.001 comparing KO1/KO2 with control, p < 0.01 comparing KO1/KO2 with rescue using one-way ANOVA. Refers to Figure 3B and Videos 5–7.DOI:http://dx.doi.org/10.7554/eLife.07389.009
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4538364&req=5

fig3s1: Rate of histoblast nest expansion measured from time-lapse videos.Each data point corresponds to one histoblast nest. The leading edge of each histoblast nest was tracked using imageJ. Speed was calculated measuring total distance covered (micrometer/hour). Genotypes: Control was esg-GAL4, UAS-GFP. esg-GAL4 and UAS-GFP were recombined onto the KO1 and KO2 chromosomes and onto the miR-965-Rescue chromosome. Data include examples with both recombinant mutant chromosomes. No difference between these two recombinants was apparent. n = 18 for the miR-965 (KO1/KO2) mutant combination. n = 15 for control and rescue. Left panel: p < 0.0001 comparing KO1/KO2 with control, p < 0.01 comparing KO1/KO2 with rescue using one-way ANOVA. Right panel: p < 0.001 comparing KO1/KO2 with control, p < 0.01 comparing KO1/KO2 with rescue using one-way ANOVA. Refers to Figure 3B and Videos 5–7.DOI:http://dx.doi.org/10.7554/eLife.07389.009
Mentions: Subsequently, the rate of histoblast nest expansion was slower in the miR-965 mutant, compared to the controls (Figure 3B). Expansion of the histoblast nests was quantified in segments 3 and 4 by monitoring the speed of migration (Figure 3—figure supplement 1, Videos 5, 6). The average speed of migration of third and fourth histoblast nests in the control samples was 15 µm/hr, compare with ∼6.5 µm/hr in the mutant. The rate of nest expansion was increased to 12–14 µm/hr by restoring miR-965 expression with the rescue allele the miR-965 mutant background (Video 7).Video 5.Control, growth phase.

Bottom Line: During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis.Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis.By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular and Cell Biology, Singapore, Singapore.

ABSTRACT
Formation of the Drosophila adult abdomen involves a process of tissue replacement in which larval epidermal cells are replaced by adult cells. The progenitors of the adult epidermis are specified during embryogenesis and, unlike the imaginal discs that make up the thoracic and head segments, they remain quiescent during larval development. During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis. Here, we provide evidence that the microRNA, miR-965, acts via string and wingless to control histoblast proliferation and migration. Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis. Replacement of the larval epidermis by adult epidermal progenitors involves regulation of both cell-intrinsic events and cell communication. By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

No MeSH data available.


Related in: MedlinePlus