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miR-965 controls cell proliferation and migration during tissue morphogenesis in the Drosophila abdomen.

Verma P, Cohen SM - Elife (2015)

Bottom Line: During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis.Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis.By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular and Cell Biology, Singapore, Singapore.

ABSTRACT
Formation of the Drosophila adult abdomen involves a process of tissue replacement in which larval epidermal cells are replaced by adult cells. The progenitors of the adult epidermis are specified during embryogenesis and, unlike the imaginal discs that make up the thoracic and head segments, they remain quiescent during larval development. During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis. Here, we provide evidence that the microRNA, miR-965, acts via string and wingless to control histoblast proliferation and migration. Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis. Replacement of the larval epidermis by adult epidermal progenitors involves regulation of both cell-intrinsic events and cell communication. By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

No MeSH data available.


Related in: MedlinePlus

Evidence that kismet and miR-965 arise from a common transcription unit.Above: diagram of the kismet locus showing miR-965 in the first intron. kismet and miR-965 are transcribed in the same direction. Below: quantitative miRNA PCR showing the level of miR-965 miRNA. miR-965 levels were reduced in flies carrying several kismet alleles associated with different transgene insertions near the first exon, in which both copies of the miRNA gene should be intact. Genotypes are shown at left and the expected number of copies of miR-965 DNA is shown at right. miR-965 levels were somewhat lower than the expected 50% in the KO/+ heterozygote. ‘B’ indicates balancer chromosome. Refers to Figure 1A–D.DOI:http://dx.doi.org/10.7554/eLife.07389.004
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fig1s1: Evidence that kismet and miR-965 arise from a common transcription unit.Above: diagram of the kismet locus showing miR-965 in the first intron. kismet and miR-965 are transcribed in the same direction. Below: quantitative miRNA PCR showing the level of miR-965 miRNA. miR-965 levels were reduced in flies carrying several kismet alleles associated with different transgene insertions near the first exon, in which both copies of the miRNA gene should be intact. Genotypes are shown at left and the expected number of copies of miR-965 DNA is shown at right. miR-965 levels were somewhat lower than the expected 50% in the KO/+ heterozygote. ‘B’ indicates balancer chromosome. Refers to Figure 1A–D.DOI:http://dx.doi.org/10.7554/eLife.07389.004

Mentions: The miR-965 microRNA is located in the first intron of the kismet gene (Figure 1A). kismet and miR-965 are transcribed in the same direction. Quantitative PCR showed that a series of P-element insertional mutants near the first exon of the kismet gene reduced the level of mature miR-965 miRNA, suggesting that kismet and miR-965 arise from a single transcription unit (Figure 1—figure supplement 1).10.7554/eLife.07389.003Figure 1.The miR-965 mutant.


miR-965 controls cell proliferation and migration during tissue morphogenesis in the Drosophila abdomen.

Verma P, Cohen SM - Elife (2015)

Evidence that kismet and miR-965 arise from a common transcription unit.Above: diagram of the kismet locus showing miR-965 in the first intron. kismet and miR-965 are transcribed in the same direction. Below: quantitative miRNA PCR showing the level of miR-965 miRNA. miR-965 levels were reduced in flies carrying several kismet alleles associated with different transgene insertions near the first exon, in which both copies of the miRNA gene should be intact. Genotypes are shown at left and the expected number of copies of miR-965 DNA is shown at right. miR-965 levels were somewhat lower than the expected 50% in the KO/+ heterozygote. ‘B’ indicates balancer chromosome. Refers to Figure 1A–D.DOI:http://dx.doi.org/10.7554/eLife.07389.004
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4538364&req=5

fig1s1: Evidence that kismet and miR-965 arise from a common transcription unit.Above: diagram of the kismet locus showing miR-965 in the first intron. kismet and miR-965 are transcribed in the same direction. Below: quantitative miRNA PCR showing the level of miR-965 miRNA. miR-965 levels were reduced in flies carrying several kismet alleles associated with different transgene insertions near the first exon, in which both copies of the miRNA gene should be intact. Genotypes are shown at left and the expected number of copies of miR-965 DNA is shown at right. miR-965 levels were somewhat lower than the expected 50% in the KO/+ heterozygote. ‘B’ indicates balancer chromosome. Refers to Figure 1A–D.DOI:http://dx.doi.org/10.7554/eLife.07389.004
Mentions: The miR-965 microRNA is located in the first intron of the kismet gene (Figure 1A). kismet and miR-965 are transcribed in the same direction. Quantitative PCR showed that a series of P-element insertional mutants near the first exon of the kismet gene reduced the level of mature miR-965 miRNA, suggesting that kismet and miR-965 arise from a single transcription unit (Figure 1—figure supplement 1).10.7554/eLife.07389.003Figure 1.The miR-965 mutant.

Bottom Line: During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis.Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis.By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular and Cell Biology, Singapore, Singapore.

ABSTRACT
Formation of the Drosophila adult abdomen involves a process of tissue replacement in which larval epidermal cells are replaced by adult cells. The progenitors of the adult epidermis are specified during embryogenesis and, unlike the imaginal discs that make up the thoracic and head segments, they remain quiescent during larval development. During pupal development, the abdominal histoblast cells proliferate and migrate to replace the larval epidermis. Here, we provide evidence that the microRNA, miR-965, acts via string and wingless to control histoblast proliferation and migration. Ecdysone signaling downregulates miR-965 at the onset of pupariation, linking activation of the histoblast nests to the hormonal control of metamorphosis. Replacement of the larval epidermis by adult epidermal progenitors involves regulation of both cell-intrinsic events and cell communication. By regulating both cell proliferation and cell migration, miR-965 contributes to the robustness of this morphogenetic system.

No MeSH data available.


Related in: MedlinePlus