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Heterogeneity of Genetic Damage in Cervical Nuclei and Lymphocytes in Women with Different Levels of Dysplasia and Cancer-Associated Risk Factors.

Alvarez-Moya C, Reynoso-Silva M, Canales-Aguirre AA, Chavez-Chavez JO, Castañeda-Vázquez H, Feria-Velasco AI - Biomed Res Int (2015)

Bottom Line: The comet assay can be used to assess genetic damage, but heterogeneity in the length of the tails is frequently observed.A comet assay was performed in peripheral blood lymphocytes and cervical epithelial cells.Results suggest that genetic damage could be widely present but only manifested as increased tail length in certain cell populations.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Biology Department, Guadalajara University, Juarez 976, Colonia Centro, 44100 Guadalajara, JAL, Mexico.

ABSTRACT
The comet assay can be used to assess genetic damage, but heterogeneity in the length of the tails is frequently observed. The aims of this study were to evaluate genetic damage and heterogeneity in the cervical nuclei and lymphocytes from patients with different levels of dysplasia and to determine the risk factors associated with the development of cervical cancer. The study included 97 females who presented with different levels of dysplasia. A comet assay was performed in peripheral blood lymphocytes and cervical epithelial cells. Significant genetic damage (P ≤ 0.05) was observed only in patients diagnosed with nuclei cervical from dysplasia III (NCDIII) and lymphocytes from dysplasia I (LDI). However, the standard deviations of the tail lengths in the cervical nuclei and lymphocytes from patients with dysplasia I were significantly different (P ≤ 0.0001) from the standard deviations of the tail lengths in the nuclei cervical and lymphocytes from patients with DII and DIII (NCDII, NCDIII and LDII, LDIII), indicating a high heterogeneity in tail length. Results suggest that genetic damage could be widely present but only manifested as increased tail length in certain cell populations. This heterogeneity could obscure the statistical significance of the genetic damage.

No MeSH data available.


Related in: MedlinePlus

Average tail length (genetic damage) of cervical nuclei and lymphocytes from women with different grades of dysplasia. The number in the bars corresponds to the average tail length of cervical cells or nuclei from women with a certain level of dysplasia. Cervical cells and nuclei were placed separately. The negative control is also visualized. *P ≤ 0.05.  *Bartlett's test suggests that the SD in both cervical tissue nuclei and lymphocytes from cervical dysplasia I are significantly different (P ≤ 0.0001) from those of lymphocytes and cervical tissue nuclei from dysplasia II and III.
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fig2: Average tail length (genetic damage) of cervical nuclei and lymphocytes from women with different grades of dysplasia. The number in the bars corresponds to the average tail length of cervical cells or nuclei from women with a certain level of dysplasia. Cervical cells and nuclei were placed separately. The negative control is also visualized. *P ≤ 0.05.  *Bartlett's test suggests that the SD in both cervical tissue nuclei and lymphocytes from cervical dysplasia I are significantly different (P ≤ 0.0001) from those of lymphocytes and cervical tissue nuclei from dysplasia II and III.

Mentions: Averages of the DNA migration for both the lymphocytes and the cervical nuclei were obtained and are presented in Figure 2 and the comparisons with the corresponding negative controls were statistically significant (P ≤ 0.05) only for NCDIII and LDI (Figure 2). However, the standard deviations of the cervical nuclei and lymphocytes from the dysplasia I group were significantly different (P ≤ 0.0001) from the cervical nuclei and lymphocytes from the DII and DIII groups. The comparison between negative controls showed no significant difference.


Heterogeneity of Genetic Damage in Cervical Nuclei and Lymphocytes in Women with Different Levels of Dysplasia and Cancer-Associated Risk Factors.

Alvarez-Moya C, Reynoso-Silva M, Canales-Aguirre AA, Chavez-Chavez JO, Castañeda-Vázquez H, Feria-Velasco AI - Biomed Res Int (2015)

Average tail length (genetic damage) of cervical nuclei and lymphocytes from women with different grades of dysplasia. The number in the bars corresponds to the average tail length of cervical cells or nuclei from women with a certain level of dysplasia. Cervical cells and nuclei were placed separately. The negative control is also visualized. *P ≤ 0.05.  *Bartlett's test suggests that the SD in both cervical tissue nuclei and lymphocytes from cervical dysplasia I are significantly different (P ≤ 0.0001) from those of lymphocytes and cervical tissue nuclei from dysplasia II and III.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538336&req=5

fig2: Average tail length (genetic damage) of cervical nuclei and lymphocytes from women with different grades of dysplasia. The number in the bars corresponds to the average tail length of cervical cells or nuclei from women with a certain level of dysplasia. Cervical cells and nuclei were placed separately. The negative control is also visualized. *P ≤ 0.05.  *Bartlett's test suggests that the SD in both cervical tissue nuclei and lymphocytes from cervical dysplasia I are significantly different (P ≤ 0.0001) from those of lymphocytes and cervical tissue nuclei from dysplasia II and III.
Mentions: Averages of the DNA migration for both the lymphocytes and the cervical nuclei were obtained and are presented in Figure 2 and the comparisons with the corresponding negative controls were statistically significant (P ≤ 0.05) only for NCDIII and LDI (Figure 2). However, the standard deviations of the cervical nuclei and lymphocytes from the dysplasia I group were significantly different (P ≤ 0.0001) from the cervical nuclei and lymphocytes from the DII and DIII groups. The comparison between negative controls showed no significant difference.

Bottom Line: The comet assay can be used to assess genetic damage, but heterogeneity in the length of the tails is frequently observed.A comet assay was performed in peripheral blood lymphocytes and cervical epithelial cells.Results suggest that genetic damage could be widely present but only manifested as increased tail length in certain cell populations.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Biology Department, Guadalajara University, Juarez 976, Colonia Centro, 44100 Guadalajara, JAL, Mexico.

ABSTRACT
The comet assay can be used to assess genetic damage, but heterogeneity in the length of the tails is frequently observed. The aims of this study were to evaluate genetic damage and heterogeneity in the cervical nuclei and lymphocytes from patients with different levels of dysplasia and to determine the risk factors associated with the development of cervical cancer. The study included 97 females who presented with different levels of dysplasia. A comet assay was performed in peripheral blood lymphocytes and cervical epithelial cells. Significant genetic damage (P ≤ 0.05) was observed only in patients diagnosed with nuclei cervical from dysplasia III (NCDIII) and lymphocytes from dysplasia I (LDI). However, the standard deviations of the tail lengths in the cervical nuclei and lymphocytes from patients with dysplasia I were significantly different (P ≤ 0.0001) from the standard deviations of the tail lengths in the nuclei cervical and lymphocytes from patients with DII and DIII (NCDII, NCDIII and LDII, LDIII), indicating a high heterogeneity in tail length. Results suggest that genetic damage could be widely present but only manifested as increased tail length in certain cell populations. This heterogeneity could obscure the statistical significance of the genetic damage.

No MeSH data available.


Related in: MedlinePlus