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Considerable Variation of Antibacterial Activity of Cu Nanoparticles Suspensions Depending on the Storage Time, Dispersive Medium, and Particle Sizes.

Zakharova OV, Godymchuk AY, Gusev AA, Gulchenko SI, Vasyukova IA, Kuznetsov DV - Biomed Res Int (2015)

Bottom Line: In this study we examined copper nanoparticles of various sizes obtained by the method of wire electric explosion: nanopowder average size 50 nm (Cu 50) and 100 nm (Cu 100).Ionic solution of Cu(2+) and sodium dichloroisocyanurate was used for comparison study.It has been observed that antibacterial properties of Cu 50 nanoparticle suspensions are ceased after 24-hour storage, while for Cu 100 suspensions no correlation between antibacterial properties and storage time has been noted.

View Article: PubMed Central - PubMed

Affiliation: Tambov State University named after G.R. Derzhavin, 33 Internatsionalnaya street, Tambov 392000, Russia.

ABSTRACT
Suspensions of Cu nanoparticles are promising for creating the new class of alternative antimicrobial products. In this study we examined copper nanoparticles of various sizes obtained by the method of wire electric explosion: nanopowder average size 50 nm (Cu 50) and 100 nm (Cu 100). The paper presents the complex study of the influence of physicochemical properties such as particle size and concentration of the freshly prepared and 24-hour suspensions of Cu nanoparticles in distilled water and physiological solution upon their toxicity to bacteria E. coli M-17. Ionic solution of Cu(2+) and sodium dichloroisocyanurate was used for comparison study. It has been shown that decrease in the nanoparticle size leads to changes in the correlation between toxicity and concentration as toxicity peaks are observed at low concentrations (0.0001⋯0.01 mg/L). It has been observed that antibacterial properties of Cu 50 nanoparticle suspensions are ceased after 24-hour storage, while for Cu 100 suspensions no correlation between antibacterial properties and storage time has been noted. Cu 100 nanoparticle suspensions at 10 mg/L concentration display higher toxicity at substituting physiological solution for water than Cu 50 suspensions. Dependence of the toxicity on the mean particle aggregates size in suspension was not revealed.

No MeSH data available.


Related in: MedlinePlus

Changes in the toxicity index for Cu2+ solutions in (a) water, Cu2+ + H2O and (b) physiological solution, Cu2+ + PS.
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fig7: Changes in the toxicity index for Cu2+ solutions in (a) water, Cu2+ + H2O and (b) physiological solution, Cu2+ + PS.

Mentions: It is deduced from experiments that Cu2+ solutions with concentration ≤ 1 mg/L display no cytotoxicity (Figures 7(a) and 7(b)). High toxicity level (>90 units) is observed at 10 mg/L concentration, while storage time influence upon bactericidal effect is noted only for PS-based solutions: toxic effect disappears after 24-hour storage. Minimum inhibitory concentration (MIC) for water solutions and for fresh suspension based on physical solution is 2 mg/L. Minimum bactericidal concentration (MBC) for water solutions is 10 mg/L; for fresh suspension based on physical solution it is 10 mg/L. For 24-hour suspension based on physical solution neither MIC nor MBC were calculated.


Considerable Variation of Antibacterial Activity of Cu Nanoparticles Suspensions Depending on the Storage Time, Dispersive Medium, and Particle Sizes.

Zakharova OV, Godymchuk AY, Gusev AA, Gulchenko SI, Vasyukova IA, Kuznetsov DV - Biomed Res Int (2015)

Changes in the toxicity index for Cu2+ solutions in (a) water, Cu2+ + H2O and (b) physiological solution, Cu2+ + PS.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538334&req=5

fig7: Changes in the toxicity index for Cu2+ solutions in (a) water, Cu2+ + H2O and (b) physiological solution, Cu2+ + PS.
Mentions: It is deduced from experiments that Cu2+ solutions with concentration ≤ 1 mg/L display no cytotoxicity (Figures 7(a) and 7(b)). High toxicity level (>90 units) is observed at 10 mg/L concentration, while storage time influence upon bactericidal effect is noted only for PS-based solutions: toxic effect disappears after 24-hour storage. Minimum inhibitory concentration (MIC) for water solutions and for fresh suspension based on physical solution is 2 mg/L. Minimum bactericidal concentration (MBC) for water solutions is 10 mg/L; for fresh suspension based on physical solution it is 10 mg/L. For 24-hour suspension based on physical solution neither MIC nor MBC were calculated.

Bottom Line: In this study we examined copper nanoparticles of various sizes obtained by the method of wire electric explosion: nanopowder average size 50 nm (Cu 50) and 100 nm (Cu 100).Ionic solution of Cu(2+) and sodium dichloroisocyanurate was used for comparison study.It has been observed that antibacterial properties of Cu 50 nanoparticle suspensions are ceased after 24-hour storage, while for Cu 100 suspensions no correlation between antibacterial properties and storage time has been noted.

View Article: PubMed Central - PubMed

Affiliation: Tambov State University named after G.R. Derzhavin, 33 Internatsionalnaya street, Tambov 392000, Russia.

ABSTRACT
Suspensions of Cu nanoparticles are promising for creating the new class of alternative antimicrobial products. In this study we examined copper nanoparticles of various sizes obtained by the method of wire electric explosion: nanopowder average size 50 nm (Cu 50) and 100 nm (Cu 100). The paper presents the complex study of the influence of physicochemical properties such as particle size and concentration of the freshly prepared and 24-hour suspensions of Cu nanoparticles in distilled water and physiological solution upon their toxicity to bacteria E. coli M-17. Ionic solution of Cu(2+) and sodium dichloroisocyanurate was used for comparison study. It has been shown that decrease in the nanoparticle size leads to changes in the correlation between toxicity and concentration as toxicity peaks are observed at low concentrations (0.0001⋯0.01 mg/L). It has been observed that antibacterial properties of Cu 50 nanoparticle suspensions are ceased after 24-hour storage, while for Cu 100 suspensions no correlation between antibacterial properties and storage time has been noted. Cu 100 nanoparticle suspensions at 10 mg/L concentration display higher toxicity at substituting physiological solution for water than Cu 50 suspensions. Dependence of the toxicity on the mean particle aggregates size in suspension was not revealed.

No MeSH data available.


Related in: MedlinePlus