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ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells.

Wang C, Hu X, Gao Y, Ji Y - Biomed Res Int (2015)

Bottom Line: Both intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) were examined by a microplate-reader method.Results showed that ZnO NPs (≥ 0.01 μg/mL) significantly inhibited proliferation (P < 0.05) and induced substantial apoptosis in LTEP-a-2 cells after 4 h of exposure.The intracellular ROS level rose up to 30-40% corresponding to significant depletion (approximately 70-80%) in GSH content in LTEP-a-2 cells (P < 0.05), suggesting that ZnO NPs induced apoptosis mainly through increased ROS production.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 17 Chunhui Road, Laishan District, Yantai 264003, China ; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
Owing to the wide use of novel nanoparticles (NPs) such as zinc oxide (ZnO) in all aspects of life, toxicological research on ZnO NPs is receiving increasing attention in these days. In this study, the toxicity of ZnO NPs in a human pulmonary adenocarcinoma cell line LTEP-a-2 was tested in vitro. Log-phase cells were exposed to different levels of ZnO NPs for hours, followed by colorimetric cell viability assay using tetrazolium salt and cell survival rate assay using trypan blue dye. Cell morphological changes were observed by Giemsa staining and light microscopy. Apoptosis was detected by using fluorescence microscopy and caspase-3 activity assay. Both intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) were examined by a microplate-reader method. Results showed that ZnO NPs (≥ 0.01 μg/mL) significantly inhibited proliferation (P < 0.05) and induced substantial apoptosis in LTEP-a-2 cells after 4 h of exposure. The intracellular ROS level rose up to 30-40% corresponding to significant depletion (approximately 70-80%) in GSH content in LTEP-a-2 cells (P < 0.05), suggesting that ZnO NPs induced apoptosis mainly through increased ROS production. This study elucidates the toxicological mechanism of ZnO NPs in human pulmonary adenocarcinoma cells and provides reference data for application of nanomaterials in the environment.

No MeSH data available.


Related in: MedlinePlus

Major characteristics of ZnO nanoparticles used in this study. (a) Scanning electron micrograph, (b) size distribution, and (c) major physical properties.
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fig1: Major characteristics of ZnO nanoparticles used in this study. (a) Scanning electron micrograph, (b) size distribution, and (c) major physical properties.

Mentions: A description of the morphology and physicochemical properties of ZnO NPs is regarded as a comparative study in the field of cytotoxicity research [23, 24]. In the present study, SEM image shows that the ZnO NPs in use are mainly anxiolytic shaped and are partially rhombic (Figure 1(a)). Mean grain diameter of the ZnO NPs is 30 ± 5 nm, which matches the supplier's declaration. Zeta potential data indicate that the ZnO NPs have a positive surface charge, −18.6 mV at pH 7.4 in DMEM (Figure 1(b)), which is inadequate to stabilize the suspension of ZnO NPs via repulsive force and thus may cause NPs aggregation in DMEM. The size distribution of ZnO NPs in DMEM, as determined by dynamic light scattering, shows great variations (Figure 1(c)).


ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells.

Wang C, Hu X, Gao Y, Ji Y - Biomed Res Int (2015)

Major characteristics of ZnO nanoparticles used in this study. (a) Scanning electron micrograph, (b) size distribution, and (c) major physical properties.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538331&req=5

fig1: Major characteristics of ZnO nanoparticles used in this study. (a) Scanning electron micrograph, (b) size distribution, and (c) major physical properties.
Mentions: A description of the morphology and physicochemical properties of ZnO NPs is regarded as a comparative study in the field of cytotoxicity research [23, 24]. In the present study, SEM image shows that the ZnO NPs in use are mainly anxiolytic shaped and are partially rhombic (Figure 1(a)). Mean grain diameter of the ZnO NPs is 30 ± 5 nm, which matches the supplier's declaration. Zeta potential data indicate that the ZnO NPs have a positive surface charge, −18.6 mV at pH 7.4 in DMEM (Figure 1(b)), which is inadequate to stabilize the suspension of ZnO NPs via repulsive force and thus may cause NPs aggregation in DMEM. The size distribution of ZnO NPs in DMEM, as determined by dynamic light scattering, shows great variations (Figure 1(c)).

Bottom Line: Both intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) were examined by a microplate-reader method.Results showed that ZnO NPs (≥ 0.01 μg/mL) significantly inhibited proliferation (P < 0.05) and induced substantial apoptosis in LTEP-a-2 cells after 4 h of exposure.The intracellular ROS level rose up to 30-40% corresponding to significant depletion (approximately 70-80%) in GSH content in LTEP-a-2 cells (P < 0.05), suggesting that ZnO NPs induced apoptosis mainly through increased ROS production.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 17 Chunhui Road, Laishan District, Yantai 264003, China ; University of Chinese Academy of Sciences, Beijing 100049, China.

ABSTRACT
Owing to the wide use of novel nanoparticles (NPs) such as zinc oxide (ZnO) in all aspects of life, toxicological research on ZnO NPs is receiving increasing attention in these days. In this study, the toxicity of ZnO NPs in a human pulmonary adenocarcinoma cell line LTEP-a-2 was tested in vitro. Log-phase cells were exposed to different levels of ZnO NPs for hours, followed by colorimetric cell viability assay using tetrazolium salt and cell survival rate assay using trypan blue dye. Cell morphological changes were observed by Giemsa staining and light microscopy. Apoptosis was detected by using fluorescence microscopy and caspase-3 activity assay. Both intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) were examined by a microplate-reader method. Results showed that ZnO NPs (≥ 0.01 μg/mL) significantly inhibited proliferation (P < 0.05) and induced substantial apoptosis in LTEP-a-2 cells after 4 h of exposure. The intracellular ROS level rose up to 30-40% corresponding to significant depletion (approximately 70-80%) in GSH content in LTEP-a-2 cells (P < 0.05), suggesting that ZnO NPs induced apoptosis mainly through increased ROS production. This study elucidates the toxicological mechanism of ZnO NPs in human pulmonary adenocarcinoma cells and provides reference data for application of nanomaterials in the environment.

No MeSH data available.


Related in: MedlinePlus