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Effect of 50 Hz Extremely Low-Frequency Electromagnetic Fields on the DNA Methylation and DNA Methyltransferases in Mouse Spermatocyte-Derived Cell Line GC-2.

Liu Y, Liu WB, Liu KJ, Ao L, Zhong JL, Cao J, Liu JY - Biomed Res Int (2015)

Bottom Line: However, the biological effects and molecular mechanism are largely unclear.Our study was designed to elucidate the epigenetic effects of 50 Hz ELF-EMF in vitro.The expression of DNMT1 and DNMT3b was decreased at 1 mT, and 50 Hz ELF-EMF can increase the expression of DNMT1 and DNMT3b of GC-2 cells at 3 mT.

View Article: PubMed Central - PubMed

Affiliation: College of Bioengineering, Chongqing University, Chongqing 400044, China ; Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
Previous studies have shown that the male reproductive system is one of the most sensitive organs to electromagnetic radiation. However, the biological effects and molecular mechanism are largely unclear. Our study was designed to elucidate the epigenetic effects of 50 Hz ELF-EMF in vitro. Mouse spermatocyte-derived GC-2 cell line was exposed to 50 Hz ELF-EMF (5 min on and 10 min off) at magnetic field intensity of 1 mT, 2 mT, and 3 mT with an intermittent exposure for 72 h. We found that 50 Hz ELF-EMF exposure decreased genome-wide methylation at 1 mT, but global methylation was higher at 3 mT compared with the controls. The expression of DNMT1 and DNMT3b was decreased at 1 mT, and 50 Hz ELF-EMF can increase the expression of DNMT1 and DNMT3b of GC-2 cells at 3 mT. However, 50 Hz ELF-EMF had little influence on the expression of DNMT3a. Then, we established DNA methylation and gene expression profiling and validated some genes with aberrant DNA methylation and expression at different intensity of 50 Hz ELF-EMF. These results suggest that the alterations of genome-wide methylation and DNMTs expression may play an important role in the biological effects of 50 Hz ELF-EMF exposure.

No MeSH data available.


Related in: MedlinePlus

Network analysis of dynamic gene expression in GC-2 cells at magnetic field intensity of 1 mT (a) and 3 mT (b). The red dot stands for upregulated genes, the blue dot is downregulated genes, and the lilac dot stands for the connection gene.
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fig5: Network analysis of dynamic gene expression in GC-2 cells at magnetic field intensity of 1 mT (a) and 3 mT (b). The red dot stands for upregulated genes, the blue dot is downregulated genes, and the lilac dot stands for the connection gene.

Mentions: To better elucidate the molecular mechanisms and biological pathways implicated in 50 Hz ELF-EMF exposure in GC-2 cells, network analyses were used to find the differentially expressed and target key genes in GC-2 cells between the exposure group and the sham-exposure group. Network analyses were generated through the use of the software confirmed the major functionally related gene groups. As seen from Figure 5(a), Maoa, Bhmt, Gng8, Ugt2b34, Dgat1, and Adh1 may be target genes in GC-2 cells at magnetic intensity of 1 mT. Cyp3a11, Ugt2b34, Adcy5, Ptgs1, and Cyp2b23 may be closely related with the epigenetic of 50 Hz ELF-EMF exposure at magnetic intensity of 3 mT (Figure 5(b)).


Effect of 50 Hz Extremely Low-Frequency Electromagnetic Fields on the DNA Methylation and DNA Methyltransferases in Mouse Spermatocyte-Derived Cell Line GC-2.

Liu Y, Liu WB, Liu KJ, Ao L, Zhong JL, Cao J, Liu JY - Biomed Res Int (2015)

Network analysis of dynamic gene expression in GC-2 cells at magnetic field intensity of 1 mT (a) and 3 mT (b). The red dot stands for upregulated genes, the blue dot is downregulated genes, and the lilac dot stands for the connection gene.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538330&req=5

fig5: Network analysis of dynamic gene expression in GC-2 cells at magnetic field intensity of 1 mT (a) and 3 mT (b). The red dot stands for upregulated genes, the blue dot is downregulated genes, and the lilac dot stands for the connection gene.
Mentions: To better elucidate the molecular mechanisms and biological pathways implicated in 50 Hz ELF-EMF exposure in GC-2 cells, network analyses were used to find the differentially expressed and target key genes in GC-2 cells between the exposure group and the sham-exposure group. Network analyses were generated through the use of the software confirmed the major functionally related gene groups. As seen from Figure 5(a), Maoa, Bhmt, Gng8, Ugt2b34, Dgat1, and Adh1 may be target genes in GC-2 cells at magnetic intensity of 1 mT. Cyp3a11, Ugt2b34, Adcy5, Ptgs1, and Cyp2b23 may be closely related with the epigenetic of 50 Hz ELF-EMF exposure at magnetic intensity of 3 mT (Figure 5(b)).

Bottom Line: However, the biological effects and molecular mechanism are largely unclear.Our study was designed to elucidate the epigenetic effects of 50 Hz ELF-EMF in vitro.The expression of DNMT1 and DNMT3b was decreased at 1 mT, and 50 Hz ELF-EMF can increase the expression of DNMT1 and DNMT3b of GC-2 cells at 3 mT.

View Article: PubMed Central - PubMed

Affiliation: College of Bioengineering, Chongqing University, Chongqing 400044, China ; Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
Previous studies have shown that the male reproductive system is one of the most sensitive organs to electromagnetic radiation. However, the biological effects and molecular mechanism are largely unclear. Our study was designed to elucidate the epigenetic effects of 50 Hz ELF-EMF in vitro. Mouse spermatocyte-derived GC-2 cell line was exposed to 50 Hz ELF-EMF (5 min on and 10 min off) at magnetic field intensity of 1 mT, 2 mT, and 3 mT with an intermittent exposure for 72 h. We found that 50 Hz ELF-EMF exposure decreased genome-wide methylation at 1 mT, but global methylation was higher at 3 mT compared with the controls. The expression of DNMT1 and DNMT3b was decreased at 1 mT, and 50 Hz ELF-EMF can increase the expression of DNMT1 and DNMT3b of GC-2 cells at 3 mT. However, 50 Hz ELF-EMF had little influence on the expression of DNMT3a. Then, we established DNA methylation and gene expression profiling and validated some genes with aberrant DNA methylation and expression at different intensity of 50 Hz ELF-EMF. These results suggest that the alterations of genome-wide methylation and DNMTs expression may play an important role in the biological effects of 50 Hz ELF-EMF exposure.

No MeSH data available.


Related in: MedlinePlus