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Effect of 50 Hz Extremely Low-Frequency Electromagnetic Fields on the DNA Methylation and DNA Methyltransferases in Mouse Spermatocyte-Derived Cell Line GC-2.

Liu Y, Liu WB, Liu KJ, Ao L, Zhong JL, Cao J, Liu JY - Biomed Res Int (2015)

Bottom Line: However, the biological effects and molecular mechanism are largely unclear.Our study was designed to elucidate the epigenetic effects of 50 Hz ELF-EMF in vitro.The expression of DNMT1 and DNMT3b was decreased at 1 mT, and 50 Hz ELF-EMF can increase the expression of DNMT1 and DNMT3b of GC-2 cells at 3 mT.

View Article: PubMed Central - PubMed

Affiliation: College of Bioengineering, Chongqing University, Chongqing 400044, China ; Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
Previous studies have shown that the male reproductive system is one of the most sensitive organs to electromagnetic radiation. However, the biological effects and molecular mechanism are largely unclear. Our study was designed to elucidate the epigenetic effects of 50 Hz ELF-EMF in vitro. Mouse spermatocyte-derived GC-2 cell line was exposed to 50 Hz ELF-EMF (5 min on and 10 min off) at magnetic field intensity of 1 mT, 2 mT, and 3 mT with an intermittent exposure for 72 h. We found that 50 Hz ELF-EMF exposure decreased genome-wide methylation at 1 mT, but global methylation was higher at 3 mT compared with the controls. The expression of DNMT1 and DNMT3b was decreased at 1 mT, and 50 Hz ELF-EMF can increase the expression of DNMT1 and DNMT3b of GC-2 cells at 3 mT. However, 50 Hz ELF-EMF had little influence on the expression of DNMT3a. Then, we established DNA methylation and gene expression profiling and validated some genes with aberrant DNA methylation and expression at different intensity of 50 Hz ELF-EMF. These results suggest that the alterations of genome-wide methylation and DNMTs expression may play an important role in the biological effects of 50 Hz ELF-EMF exposure.

No MeSH data available.


Related in: MedlinePlus

Quantitative analysis of global DNA methylation in GC-2 cells. DNA methylation in GC-2 cells was lower than the sham-exposure group at magnetic field intensity of 1 mT and was higher than the sham-exposure group at 2 mT and 3 mT exposure to 50 Hz ELF-EMF.
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fig1: Quantitative analysis of global DNA methylation in GC-2 cells. DNA methylation in GC-2 cells was lower than the sham-exposure group at magnetic field intensity of 1 mT and was higher than the sham-exposure group at 2 mT and 3 mT exposure to 50 Hz ELF-EMF.

Mentions: As male reproductive system is one of the most sensitive organs to electromagnetic radiation, we sought to determine whether there is a disruption of the DNA methylation patterns in GC-2 cells exposed to 50 Hz ELF-EMF exposure for 72 h compared with the sham-exposure group. Quantitative analysis of the global DNA methylation levels showed that the DNA methylation in GC-2 cells were lower than the sham-exposure group at magnetic field intensity of 1 mT and were higher than the sham-exposure group at magnetic intensity of 2 mT and 3 mT (Figure 1). These data showed that the DNA in GC-2 cells acquired aberrant methylation pattern exposed to 50 Hz ELF-EMF exposure. It suggested that global methylation alterations might play a vital role in the biological effects and damage mechanism of 50 Hz ELF-EMF exposure.


Effect of 50 Hz Extremely Low-Frequency Electromagnetic Fields on the DNA Methylation and DNA Methyltransferases in Mouse Spermatocyte-Derived Cell Line GC-2.

Liu Y, Liu WB, Liu KJ, Ao L, Zhong JL, Cao J, Liu JY - Biomed Res Int (2015)

Quantitative analysis of global DNA methylation in GC-2 cells. DNA methylation in GC-2 cells was lower than the sham-exposure group at magnetic field intensity of 1 mT and was higher than the sham-exposure group at 2 mT and 3 mT exposure to 50 Hz ELF-EMF.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538330&req=5

fig1: Quantitative analysis of global DNA methylation in GC-2 cells. DNA methylation in GC-2 cells was lower than the sham-exposure group at magnetic field intensity of 1 mT and was higher than the sham-exposure group at 2 mT and 3 mT exposure to 50 Hz ELF-EMF.
Mentions: As male reproductive system is one of the most sensitive organs to electromagnetic radiation, we sought to determine whether there is a disruption of the DNA methylation patterns in GC-2 cells exposed to 50 Hz ELF-EMF exposure for 72 h compared with the sham-exposure group. Quantitative analysis of the global DNA methylation levels showed that the DNA methylation in GC-2 cells were lower than the sham-exposure group at magnetic field intensity of 1 mT and were higher than the sham-exposure group at magnetic intensity of 2 mT and 3 mT (Figure 1). These data showed that the DNA in GC-2 cells acquired aberrant methylation pattern exposed to 50 Hz ELF-EMF exposure. It suggested that global methylation alterations might play a vital role in the biological effects and damage mechanism of 50 Hz ELF-EMF exposure.

Bottom Line: However, the biological effects and molecular mechanism are largely unclear.Our study was designed to elucidate the epigenetic effects of 50 Hz ELF-EMF in vitro.The expression of DNMT1 and DNMT3b was decreased at 1 mT, and 50 Hz ELF-EMF can increase the expression of DNMT1 and DNMT3b of GC-2 cells at 3 mT.

View Article: PubMed Central - PubMed

Affiliation: College of Bioengineering, Chongqing University, Chongqing 400044, China ; Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
Previous studies have shown that the male reproductive system is one of the most sensitive organs to electromagnetic radiation. However, the biological effects and molecular mechanism are largely unclear. Our study was designed to elucidate the epigenetic effects of 50 Hz ELF-EMF in vitro. Mouse spermatocyte-derived GC-2 cell line was exposed to 50 Hz ELF-EMF (5 min on and 10 min off) at magnetic field intensity of 1 mT, 2 mT, and 3 mT with an intermittent exposure for 72 h. We found that 50 Hz ELF-EMF exposure decreased genome-wide methylation at 1 mT, but global methylation was higher at 3 mT compared with the controls. The expression of DNMT1 and DNMT3b was decreased at 1 mT, and 50 Hz ELF-EMF can increase the expression of DNMT1 and DNMT3b of GC-2 cells at 3 mT. However, 50 Hz ELF-EMF had little influence on the expression of DNMT3a. Then, we established DNA methylation and gene expression profiling and validated some genes with aberrant DNA methylation and expression at different intensity of 50 Hz ELF-EMF. These results suggest that the alterations of genome-wide methylation and DNMTs expression may play an important role in the biological effects of 50 Hz ELF-EMF exposure.

No MeSH data available.


Related in: MedlinePlus