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Prenatal Exposure to Sodium Arsenite Alters Placental Glucose 1, 3, and 4 Transporters in Balb/c Mice.

Gutiérrez-Torres DS, González-Horta C, Del Razo LM, Infante-Ramírez R, Ramos-Martínez E, Levario-Carrillo M, Sánchez-Ramírez B - Biomed Res Int (2015)

Bottom Line: Whereas GLUT1 expression was unchanged in placentas from exposed group, GLUT3 expression was found increased.In contrast, GLUT4 expression was significantly lower (P < 0.05) in placentas from females exposed to 12 ppm.The decrease in placental GLUT4 expression might affect the provision of adequate fetal nutrition and explain the low fetal weight observed in the exposed groups.

View Article: PubMed Central - PubMed

Affiliation: Programa de Maestría en Ciencias en Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito No. 1 Nuevo Campus Universitario, 31125 Chihuahua, CHIH, Mexico.

ABSTRACT
Inorganic arsenic (iAs) exposure induces a decrease in glucose type 4 transporter (GLUT4) expression on the adipocyte membrane, which may be related to premature births and low birth weight infants in women exposed to iAs at reproductive age. The aim of this study was to analyze the effect of sodium arsenite (NaAsO2) exposure on GLUT1, GLUT3, and GLUT4 protein expression and on placental morphology. Female Balb/c mice (n = 15) were exposed to 0, 12, and 20 ppm of NaAsO2 in drinking water from 8th to 18th day of gestation. Morphological changes and GLUT1, GLUT3, and GLUT4 expression were evaluated in placentas by immunohistochemical and image analysis and correlated with iAs and arsenical species concentration, which were quantified by atomic absorption spectroscopy. NaAsO2 exposure induced a significant decrease in fetal and placental weight (P < 0.01) and increases in infarctions and vascular congestion. Whereas GLUT1 expression was unchanged in placentas from exposed group, GLUT3 expression was found increased. In contrast, GLUT4 expression was significantly lower (P < 0.05) in placentas from females exposed to 12 ppm. The decrease in placental GLUT4 expression might affect the provision of adequate fetal nutrition and explain the low fetal weight observed in the exposed groups.

No MeSH data available.


Related in: MedlinePlus

Expression of GLUT1, 3, and 4 in placenta from female mice exposed during gestation to NaAsO2 through water. Each bar indicates the mean ± s.d. of arbitrary units of optical density (ADU) obtained for each transporter (n = 300 for each protein), according to NaAsO2 water concentration: White, 0 ppm; Gray, 12 ppm; and Black, 20 ppm. Comparisons among groups were performed using the Wilcoxon range test; ∗P < 0.05.
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fig3: Expression of GLUT1, 3, and 4 in placenta from female mice exposed during gestation to NaAsO2 through water. Each bar indicates the mean ± s.d. of arbitrary units of optical density (ADU) obtained for each transporter (n = 300 for each protein), according to NaAsO2 water concentration: White, 0 ppm; Gray, 12 ppm; and Black, 20 ppm. Comparisons among groups were performed using the Wilcoxon range test; ∗P < 0.05.

Mentions: To analyze the expression of GLUT transporters among groups, image analysis was performed using the methodology previously reported by our research group [30, 31]. The expression of GLUT1 showed no difference between placentas in female controls and those that were exposed to iAs (Figure 3). Although a nonsignificant increase was detected in GLUT3 expression in placentas from female mice exposed to 12 ppm of NaAsO2, the expression of this transporter was quite similar in placentas from females exposed to 20 ppm and in nonexposed females.


Prenatal Exposure to Sodium Arsenite Alters Placental Glucose 1, 3, and 4 Transporters in Balb/c Mice.

Gutiérrez-Torres DS, González-Horta C, Del Razo LM, Infante-Ramírez R, Ramos-Martínez E, Levario-Carrillo M, Sánchez-Ramírez B - Biomed Res Int (2015)

Expression of GLUT1, 3, and 4 in placenta from female mice exposed during gestation to NaAsO2 through water. Each bar indicates the mean ± s.d. of arbitrary units of optical density (ADU) obtained for each transporter (n = 300 for each protein), according to NaAsO2 water concentration: White, 0 ppm; Gray, 12 ppm; and Black, 20 ppm. Comparisons among groups were performed using the Wilcoxon range test; ∗P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig3: Expression of GLUT1, 3, and 4 in placenta from female mice exposed during gestation to NaAsO2 through water. Each bar indicates the mean ± s.d. of arbitrary units of optical density (ADU) obtained for each transporter (n = 300 for each protein), according to NaAsO2 water concentration: White, 0 ppm; Gray, 12 ppm; and Black, 20 ppm. Comparisons among groups were performed using the Wilcoxon range test; ∗P < 0.05.
Mentions: To analyze the expression of GLUT transporters among groups, image analysis was performed using the methodology previously reported by our research group [30, 31]. The expression of GLUT1 showed no difference between placentas in female controls and those that were exposed to iAs (Figure 3). Although a nonsignificant increase was detected in GLUT3 expression in placentas from female mice exposed to 12 ppm of NaAsO2, the expression of this transporter was quite similar in placentas from females exposed to 20 ppm and in nonexposed females.

Bottom Line: Whereas GLUT1 expression was unchanged in placentas from exposed group, GLUT3 expression was found increased.In contrast, GLUT4 expression was significantly lower (P < 0.05) in placentas from females exposed to 12 ppm.The decrease in placental GLUT4 expression might affect the provision of adequate fetal nutrition and explain the low fetal weight observed in the exposed groups.

View Article: PubMed Central - PubMed

Affiliation: Programa de Maestría en Ciencias en Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito No. 1 Nuevo Campus Universitario, 31125 Chihuahua, CHIH, Mexico.

ABSTRACT
Inorganic arsenic (iAs) exposure induces a decrease in glucose type 4 transporter (GLUT4) expression on the adipocyte membrane, which may be related to premature births and low birth weight infants in women exposed to iAs at reproductive age. The aim of this study was to analyze the effect of sodium arsenite (NaAsO2) exposure on GLUT1, GLUT3, and GLUT4 protein expression and on placental morphology. Female Balb/c mice (n = 15) were exposed to 0, 12, and 20 ppm of NaAsO2 in drinking water from 8th to 18th day of gestation. Morphological changes and GLUT1, GLUT3, and GLUT4 expression were evaluated in placentas by immunohistochemical and image analysis and correlated with iAs and arsenical species concentration, which were quantified by atomic absorption spectroscopy. NaAsO2 exposure induced a significant decrease in fetal and placental weight (P < 0.01) and increases in infarctions and vascular congestion. Whereas GLUT1 expression was unchanged in placentas from exposed group, GLUT3 expression was found increased. In contrast, GLUT4 expression was significantly lower (P < 0.05) in placentas from females exposed to 12 ppm. The decrease in placental GLUT4 expression might affect the provision of adequate fetal nutrition and explain the low fetal weight observed in the exposed groups.

No MeSH data available.


Related in: MedlinePlus