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Prenatal Exposure to Sodium Arsenite Alters Placental Glucose 1, 3, and 4 Transporters in Balb/c Mice.

Gutiérrez-Torres DS, González-Horta C, Del Razo LM, Infante-Ramírez R, Ramos-Martínez E, Levario-Carrillo M, Sánchez-Ramírez B - Biomed Res Int (2015)

Bottom Line: Whereas GLUT1 expression was unchanged in placentas from exposed group, GLUT3 expression was found increased.In contrast, GLUT4 expression was significantly lower (P < 0.05) in placentas from females exposed to 12 ppm.The decrease in placental GLUT4 expression might affect the provision of adequate fetal nutrition and explain the low fetal weight observed in the exposed groups.

View Article: PubMed Central - PubMed

Affiliation: Programa de Maestría en Ciencias en Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito No. 1 Nuevo Campus Universitario, 31125 Chihuahua, CHIH, Mexico.

ABSTRACT
Inorganic arsenic (iAs) exposure induces a decrease in glucose type 4 transporter (GLUT4) expression on the adipocyte membrane, which may be related to premature births and low birth weight infants in women exposed to iAs at reproductive age. The aim of this study was to analyze the effect of sodium arsenite (NaAsO2) exposure on GLUT1, GLUT3, and GLUT4 protein expression and on placental morphology. Female Balb/c mice (n = 15) were exposed to 0, 12, and 20 ppm of NaAsO2 in drinking water from 8th to 18th day of gestation. Morphological changes and GLUT1, GLUT3, and GLUT4 expression were evaluated in placentas by immunohistochemical and image analysis and correlated with iAs and arsenical species concentration, which were quantified by atomic absorption spectroscopy. NaAsO2 exposure induced a significant decrease in fetal and placental weight (P < 0.01) and increases in infarctions and vascular congestion. Whereas GLUT1 expression was unchanged in placentas from exposed group, GLUT3 expression was found increased. In contrast, GLUT4 expression was significantly lower (P < 0.05) in placentas from females exposed to 12 ppm. The decrease in placental GLUT4 expression might affect the provision of adequate fetal nutrition and explain the low fetal weight observed in the exposed groups.

No MeSH data available.


Related in: MedlinePlus

Morphologic alterations found in placentas from female mice exposed to NaAsO2 in water. Microphotographs show the decidua (D), junction zone (JZ), and labyrinth (L) zones of murine placenta. (a) Normal morphology of murine placenta and the trophoblast giant cells (arrows) and clusters of glycogen trophoblast cells (arrowheads) are shown in JZ and near to L. Magnification 10x. (b) Infarct and deposits of fibrinoid material (FM) present in JZ and L. (c) Hemorrhagic lesion located in D zone found in placenta from a NaAsO2 exposed female. Magnification 10x. (d) Normal appearance of the L zone present in placenta from a nonexposed female. Magnification 60x. (e) Vascular congestion (VC) located in L zone present in the placenta from a NaAsO2 exposed female. Magnification 60x. (f) Magnification of fibrinoid material (FM) in the placenta from a female exposed to NaAsO2. Magnification 60x. H&E stain.
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fig1: Morphologic alterations found in placentas from female mice exposed to NaAsO2 in water. Microphotographs show the decidua (D), junction zone (JZ), and labyrinth (L) zones of murine placenta. (a) Normal morphology of murine placenta and the trophoblast giant cells (arrows) and clusters of glycogen trophoblast cells (arrowheads) are shown in JZ and near to L. Magnification 10x. (b) Infarct and deposits of fibrinoid material (FM) present in JZ and L. (c) Hemorrhagic lesion located in D zone found in placenta from a NaAsO2 exposed female. Magnification 10x. (d) Normal appearance of the L zone present in placenta from a nonexposed female. Magnification 60x. (e) Vascular congestion (VC) located in L zone present in the placenta from a NaAsO2 exposed female. Magnification 60x. (f) Magnification of fibrinoid material (FM) in the placenta from a female exposed to NaAsO2. Magnification 60x. H&E stain.

Mentions: The morphological analysis of placentas from nonexposed females showed normal cellular architecture (Figure 1(a)); no microscopic alterations were observed in the labyrinth (Figure 1(d)). However, fibrosis and hemorrhagic processes were observed in the decidua basalis area. In placentas from NaAsO2 exposed females, it was common to detect hemorrhagic zones (Figure 1(c)) and infarct lesions in decidua basalis, where the cells were replaced by fibrinoid material (Figure 1(b)). In the labyrinth zone, vascular congestion was evident (Figure 1(e)) in placentas from females in the groups exposed to any concentration of NaAsO2. Microinfarction with cellular infiltration was detected in the labyrinth in one placenta from a NaAsO2 exposed female (Figure 1(f)).


Prenatal Exposure to Sodium Arsenite Alters Placental Glucose 1, 3, and 4 Transporters in Balb/c Mice.

Gutiérrez-Torres DS, González-Horta C, Del Razo LM, Infante-Ramírez R, Ramos-Martínez E, Levario-Carrillo M, Sánchez-Ramírez B - Biomed Res Int (2015)

Morphologic alterations found in placentas from female mice exposed to NaAsO2 in water. Microphotographs show the decidua (D), junction zone (JZ), and labyrinth (L) zones of murine placenta. (a) Normal morphology of murine placenta and the trophoblast giant cells (arrows) and clusters of glycogen trophoblast cells (arrowheads) are shown in JZ and near to L. Magnification 10x. (b) Infarct and deposits of fibrinoid material (FM) present in JZ and L. (c) Hemorrhagic lesion located in D zone found in placenta from a NaAsO2 exposed female. Magnification 10x. (d) Normal appearance of the L zone present in placenta from a nonexposed female. Magnification 60x. (e) Vascular congestion (VC) located in L zone present in the placenta from a NaAsO2 exposed female. Magnification 60x. (f) Magnification of fibrinoid material (FM) in the placenta from a female exposed to NaAsO2. Magnification 60x. H&E stain.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538324&req=5

fig1: Morphologic alterations found in placentas from female mice exposed to NaAsO2 in water. Microphotographs show the decidua (D), junction zone (JZ), and labyrinth (L) zones of murine placenta. (a) Normal morphology of murine placenta and the trophoblast giant cells (arrows) and clusters of glycogen trophoblast cells (arrowheads) are shown in JZ and near to L. Magnification 10x. (b) Infarct and deposits of fibrinoid material (FM) present in JZ and L. (c) Hemorrhagic lesion located in D zone found in placenta from a NaAsO2 exposed female. Magnification 10x. (d) Normal appearance of the L zone present in placenta from a nonexposed female. Magnification 60x. (e) Vascular congestion (VC) located in L zone present in the placenta from a NaAsO2 exposed female. Magnification 60x. (f) Magnification of fibrinoid material (FM) in the placenta from a female exposed to NaAsO2. Magnification 60x. H&E stain.
Mentions: The morphological analysis of placentas from nonexposed females showed normal cellular architecture (Figure 1(a)); no microscopic alterations were observed in the labyrinth (Figure 1(d)). However, fibrosis and hemorrhagic processes were observed in the decidua basalis area. In placentas from NaAsO2 exposed females, it was common to detect hemorrhagic zones (Figure 1(c)) and infarct lesions in decidua basalis, where the cells were replaced by fibrinoid material (Figure 1(b)). In the labyrinth zone, vascular congestion was evident (Figure 1(e)) in placentas from females in the groups exposed to any concentration of NaAsO2. Microinfarction with cellular infiltration was detected in the labyrinth in one placenta from a NaAsO2 exposed female (Figure 1(f)).

Bottom Line: Whereas GLUT1 expression was unchanged in placentas from exposed group, GLUT3 expression was found increased.In contrast, GLUT4 expression was significantly lower (P < 0.05) in placentas from females exposed to 12 ppm.The decrease in placental GLUT4 expression might affect the provision of adequate fetal nutrition and explain the low fetal weight observed in the exposed groups.

View Article: PubMed Central - PubMed

Affiliation: Programa de Maestría en Ciencias en Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito No. 1 Nuevo Campus Universitario, 31125 Chihuahua, CHIH, Mexico.

ABSTRACT
Inorganic arsenic (iAs) exposure induces a decrease in glucose type 4 transporter (GLUT4) expression on the adipocyte membrane, which may be related to premature births and low birth weight infants in women exposed to iAs at reproductive age. The aim of this study was to analyze the effect of sodium arsenite (NaAsO2) exposure on GLUT1, GLUT3, and GLUT4 protein expression and on placental morphology. Female Balb/c mice (n = 15) were exposed to 0, 12, and 20 ppm of NaAsO2 in drinking water from 8th to 18th day of gestation. Morphological changes and GLUT1, GLUT3, and GLUT4 expression were evaluated in placentas by immunohistochemical and image analysis and correlated with iAs and arsenical species concentration, which were quantified by atomic absorption spectroscopy. NaAsO2 exposure induced a significant decrease in fetal and placental weight (P < 0.01) and increases in infarctions and vascular congestion. Whereas GLUT1 expression was unchanged in placentas from exposed group, GLUT3 expression was found increased. In contrast, GLUT4 expression was significantly lower (P < 0.05) in placentas from females exposed to 12 ppm. The decrease in placental GLUT4 expression might affect the provision of adequate fetal nutrition and explain the low fetal weight observed in the exposed groups.

No MeSH data available.


Related in: MedlinePlus