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Interleukin-1 β Modulates Melatonin Secretion in Ovine Pineal Gland: Ex Vivo Study.

Herman AP, Bochenek J, Skipor J, Król K, Krawczyńska A, Antushevich H, Pawlina B, Marciniak E, Tomaszewska-Zaremba D - Biomed Res Int (2015)

Bottom Line: Animals were euthanized three hours after the injection.It was found that IL-1β abolished (P < 0.05) NE-induced increase in melatonin release.Treatment with IL-1β also reduced (P < 0.05) expression of AA-NAT enzyme compared to NE-treated explants.

View Article: PubMed Central - PubMed

Affiliation: The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Street, 05-110 Jabłonna, Poland.

ABSTRACT
The study was designed to determine the effect of proinflammatory cytokine, interleukin- (IL-) 1β, on melatonin release and expression enzymes essential for this hormone synthesis: arylalkylamine-N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT) in ovine pineal gland, taking into account the immune status of animals before sacrificing. Ewes were injected by lipopolysaccharide (LPS; 400 ng/kg) or saline, two hours after sunset during short day period (December). Animals were euthanized three hours after the injection. Next, the pineal glands were collected and divided into four explants. The explants were incubated with (1) medium 199 (control explants), (2) norepinephrine (NE; 10 µM), (3) IL-1β (75 pg/mL), or (4) NE + IL-1β. It was found that IL-1β abolished (P < 0.05) NE-induced increase in melatonin release. Treatment with IL-1β also reduced (P < 0.05) expression of AA-NAT enzyme compared to NE-treated explants. There was no effect of NE or IL-1β treatment on gene expression of HIOMT; however, the pineal fragments isolated from LPS-treated animals were characterized by elevated (P < 0.05) expression of HIOMT mRNA and protein compared to the explants from saline-treated ewes. Our study proves that IL-1β suppresses melatonin secretion and its action seems to be targeted on the reduction of pineal AA-NAT protein expression.

No MeSH data available.


Related in: MedlinePlus

The effects of norepinephrine (NE, 10 μM), interleukin-1β (IL-1β, 75 pg/mL), and mixture of NE with interleukin- (IL-) 1β on IL-1β (a) and its type 1 receptor (IL-1R) (b) relative gene expression (mean ± SEM) in the pineal gland explants collected from saline- and lipopolysaccharide- (LPS-) treated ewes. Different lowercase letters indicate significant (P < 0.05) differences within the saline-treated group; different capital letters indicate differences within the LPS-treated group; asterisk designates a significant (P < 0.05) difference between the saline- and LPS-treated groups; control: no NE/IL-1β treatment.
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Related In: Results  -  Collection


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fig5: The effects of norepinephrine (NE, 10 μM), interleukin-1β (IL-1β, 75 pg/mL), and mixture of NE with interleukin- (IL-) 1β on IL-1β (a) and its type 1 receptor (IL-1R) (b) relative gene expression (mean ± SEM) in the pineal gland explants collected from saline- and lipopolysaccharide- (LPS-) treated ewes. Different lowercase letters indicate significant (P < 0.05) differences within the saline-treated group; different capital letters indicate differences within the LPS-treated group; asterisk designates a significant (P < 0.05) difference between the saline- and LPS-treated groups; control: no NE/IL-1β treatment.

Mentions: No effects of IL-1β, NE, and immune status on the length of poly(A) tail of mRNA encoding enzymes, AA-NAT and HIOMT, were found (Figure 5).


Interleukin-1 β Modulates Melatonin Secretion in Ovine Pineal Gland: Ex Vivo Study.

Herman AP, Bochenek J, Skipor J, Król K, Krawczyńska A, Antushevich H, Pawlina B, Marciniak E, Tomaszewska-Zaremba D - Biomed Res Int (2015)

The effects of norepinephrine (NE, 10 μM), interleukin-1β (IL-1β, 75 pg/mL), and mixture of NE with interleukin- (IL-) 1β on IL-1β (a) and its type 1 receptor (IL-1R) (b) relative gene expression (mean ± SEM) in the pineal gland explants collected from saline- and lipopolysaccharide- (LPS-) treated ewes. Different lowercase letters indicate significant (P < 0.05) differences within the saline-treated group; different capital letters indicate differences within the LPS-treated group; asterisk designates a significant (P < 0.05) difference between the saline- and LPS-treated groups; control: no NE/IL-1β treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538322&req=5

fig5: The effects of norepinephrine (NE, 10 μM), interleukin-1β (IL-1β, 75 pg/mL), and mixture of NE with interleukin- (IL-) 1β on IL-1β (a) and its type 1 receptor (IL-1R) (b) relative gene expression (mean ± SEM) in the pineal gland explants collected from saline- and lipopolysaccharide- (LPS-) treated ewes. Different lowercase letters indicate significant (P < 0.05) differences within the saline-treated group; different capital letters indicate differences within the LPS-treated group; asterisk designates a significant (P < 0.05) difference between the saline- and LPS-treated groups; control: no NE/IL-1β treatment.
Mentions: No effects of IL-1β, NE, and immune status on the length of poly(A) tail of mRNA encoding enzymes, AA-NAT and HIOMT, were found (Figure 5).

Bottom Line: Animals were euthanized three hours after the injection.It was found that IL-1β abolished (P < 0.05) NE-induced increase in melatonin release.Treatment with IL-1β also reduced (P < 0.05) expression of AA-NAT enzyme compared to NE-treated explants.

View Article: PubMed Central - PubMed

Affiliation: The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Street, 05-110 Jabłonna, Poland.

ABSTRACT
The study was designed to determine the effect of proinflammatory cytokine, interleukin- (IL-) 1β, on melatonin release and expression enzymes essential for this hormone synthesis: arylalkylamine-N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT) in ovine pineal gland, taking into account the immune status of animals before sacrificing. Ewes were injected by lipopolysaccharide (LPS; 400 ng/kg) or saline, two hours after sunset during short day period (December). Animals were euthanized three hours after the injection. Next, the pineal glands were collected and divided into four explants. The explants were incubated with (1) medium 199 (control explants), (2) norepinephrine (NE; 10 µM), (3) IL-1β (75 pg/mL), or (4) NE + IL-1β. It was found that IL-1β abolished (P < 0.05) NE-induced increase in melatonin release. Treatment with IL-1β also reduced (P < 0.05) expression of AA-NAT enzyme compared to NE-treated explants. There was no effect of NE or IL-1β treatment on gene expression of HIOMT; however, the pineal fragments isolated from LPS-treated animals were characterized by elevated (P < 0.05) expression of HIOMT mRNA and protein compared to the explants from saline-treated ewes. Our study proves that IL-1β suppresses melatonin secretion and its action seems to be targeted on the reduction of pineal AA-NAT protein expression.

No MeSH data available.


Related in: MedlinePlus