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Interleukin-1 β Modulates Melatonin Secretion in Ovine Pineal Gland: Ex Vivo Study.

Herman AP, Bochenek J, Skipor J, Król K, Krawczyńska A, Antushevich H, Pawlina B, Marciniak E, Tomaszewska-Zaremba D - Biomed Res Int (2015)

Bottom Line: Animals were euthanized three hours after the injection.It was found that IL-1β abolished (P < 0.05) NE-induced increase in melatonin release.Treatment with IL-1β also reduced (P < 0.05) expression of AA-NAT enzyme compared to NE-treated explants.

View Article: PubMed Central - PubMed

Affiliation: The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Street, 05-110 Jabłonna, Poland.

ABSTRACT
The study was designed to determine the effect of proinflammatory cytokine, interleukin- (IL-) 1β, on melatonin release and expression enzymes essential for this hormone synthesis: arylalkylamine-N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT) in ovine pineal gland, taking into account the immune status of animals before sacrificing. Ewes were injected by lipopolysaccharide (LPS; 400 ng/kg) or saline, two hours after sunset during short day period (December). Animals were euthanized three hours after the injection. Next, the pineal glands were collected and divided into four explants. The explants were incubated with (1) medium 199 (control explants), (2) norepinephrine (NE; 10 µM), (3) IL-1β (75 pg/mL), or (4) NE + IL-1β. It was found that IL-1β abolished (P < 0.05) NE-induced increase in melatonin release. Treatment with IL-1β also reduced (P < 0.05) expression of AA-NAT enzyme compared to NE-treated explants. There was no effect of NE or IL-1β treatment on gene expression of HIOMT; however, the pineal fragments isolated from LPS-treated animals were characterized by elevated (P < 0.05) expression of HIOMT mRNA and protein compared to the explants from saline-treated ewes. Our study proves that IL-1β suppresses melatonin secretion and its action seems to be targeted on the reduction of pineal AA-NAT protein expression.

No MeSH data available.


Related in: MedlinePlus

The effects of norepinephrine (NE, 10 μM), interleukin- (IL-) 1β (75 pg/mL), and mixture of NE with IL-1β on the poly(A) tail length of mRNAs encoding arylalkylamine-N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT) in the pineal gland explants collected from saline- and lipopolysaccharide- (LPS-) treated ewes. M: DNA ladder M50pz, numbers indicate each experimental group: (1) control, (2) NE, (3) IL-1β, and (4) NE + IL-1β. The minimum expected size of AA-NAT and HIOMT, amplified products, was 244 bp (209 bp of AA-NAT 3′ end + 35-bp oligo(dT)-anchor) and 373 bp (338 bp of HIOMT 3′ end + 35-bp oligo(dT)-anchor), respectively.
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fig4: The effects of norepinephrine (NE, 10 μM), interleukin- (IL-) 1β (75 pg/mL), and mixture of NE with IL-1β on the poly(A) tail length of mRNAs encoding arylalkylamine-N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT) in the pineal gland explants collected from saline- and lipopolysaccharide- (LPS-) treated ewes. M: DNA ladder M50pz, numbers indicate each experimental group: (1) control, (2) NE, (3) IL-1β, and (4) NE + IL-1β. The minimum expected size of AA-NAT and HIOMT, amplified products, was 244 bp (209 bp of AA-NAT 3′ end + 35-bp oligo(dT)-anchor) and 373 bp (338 bp of HIOMT 3′ end + 35-bp oligo(dT)-anchor), respectively.

Mentions: All pineal explants expressed mRNA encoding IL-1β. Moreover, in explants obtained from LPS-treated ewes, the gene expression of IL-1β was downregulated by NE (Figure 4(a)). On the other hand, IL-1β stimulated (P < 0.05) the gene expression of its type I receptor only in the explants from saline-treated ewes (Figure 4(b)). However, this stimulatory effect of IL-1β on IL-1R1 gene expression was abolished by NE treatment. No influence of IL-1β on the IL-1R1 mRNA level was found in explants from LPS-treated animals. In these groups of explants NE reduced IL-1R1 gene expression only when acting alone, because in explants coincubated with NE and IL-1β this reducing effect was completely diminished.


Interleukin-1 β Modulates Melatonin Secretion in Ovine Pineal Gland: Ex Vivo Study.

Herman AP, Bochenek J, Skipor J, Król K, Krawczyńska A, Antushevich H, Pawlina B, Marciniak E, Tomaszewska-Zaremba D - Biomed Res Int (2015)

The effects of norepinephrine (NE, 10 μM), interleukin- (IL-) 1β (75 pg/mL), and mixture of NE with IL-1β on the poly(A) tail length of mRNAs encoding arylalkylamine-N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT) in the pineal gland explants collected from saline- and lipopolysaccharide- (LPS-) treated ewes. M: DNA ladder M50pz, numbers indicate each experimental group: (1) control, (2) NE, (3) IL-1β, and (4) NE + IL-1β. The minimum expected size of AA-NAT and HIOMT, amplified products, was 244 bp (209 bp of AA-NAT 3′ end + 35-bp oligo(dT)-anchor) and 373 bp (338 bp of HIOMT 3′ end + 35-bp oligo(dT)-anchor), respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538322&req=5

fig4: The effects of norepinephrine (NE, 10 μM), interleukin- (IL-) 1β (75 pg/mL), and mixture of NE with IL-1β on the poly(A) tail length of mRNAs encoding arylalkylamine-N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT) in the pineal gland explants collected from saline- and lipopolysaccharide- (LPS-) treated ewes. M: DNA ladder M50pz, numbers indicate each experimental group: (1) control, (2) NE, (3) IL-1β, and (4) NE + IL-1β. The minimum expected size of AA-NAT and HIOMT, amplified products, was 244 bp (209 bp of AA-NAT 3′ end + 35-bp oligo(dT)-anchor) and 373 bp (338 bp of HIOMT 3′ end + 35-bp oligo(dT)-anchor), respectively.
Mentions: All pineal explants expressed mRNA encoding IL-1β. Moreover, in explants obtained from LPS-treated ewes, the gene expression of IL-1β was downregulated by NE (Figure 4(a)). On the other hand, IL-1β stimulated (P < 0.05) the gene expression of its type I receptor only in the explants from saline-treated ewes (Figure 4(b)). However, this stimulatory effect of IL-1β on IL-1R1 gene expression was abolished by NE treatment. No influence of IL-1β on the IL-1R1 mRNA level was found in explants from LPS-treated animals. In these groups of explants NE reduced IL-1R1 gene expression only when acting alone, because in explants coincubated with NE and IL-1β this reducing effect was completely diminished.

Bottom Line: Animals were euthanized three hours after the injection.It was found that IL-1β abolished (P < 0.05) NE-induced increase in melatonin release.Treatment with IL-1β also reduced (P < 0.05) expression of AA-NAT enzyme compared to NE-treated explants.

View Article: PubMed Central - PubMed

Affiliation: The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 Street, 05-110 Jabłonna, Poland.

ABSTRACT
The study was designed to determine the effect of proinflammatory cytokine, interleukin- (IL-) 1β, on melatonin release and expression enzymes essential for this hormone synthesis: arylalkylamine-N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT) in ovine pineal gland, taking into account the immune status of animals before sacrificing. Ewes were injected by lipopolysaccharide (LPS; 400 ng/kg) or saline, two hours after sunset during short day period (December). Animals were euthanized three hours after the injection. Next, the pineal glands were collected and divided into four explants. The explants were incubated with (1) medium 199 (control explants), (2) norepinephrine (NE; 10 µM), (3) IL-1β (75 pg/mL), or (4) NE + IL-1β. It was found that IL-1β abolished (P < 0.05) NE-induced increase in melatonin release. Treatment with IL-1β also reduced (P < 0.05) expression of AA-NAT enzyme compared to NE-treated explants. There was no effect of NE or IL-1β treatment on gene expression of HIOMT; however, the pineal fragments isolated from LPS-treated animals were characterized by elevated (P < 0.05) expression of HIOMT mRNA and protein compared to the explants from saline-treated ewes. Our study proves that IL-1β suppresses melatonin secretion and its action seems to be targeted on the reduction of pineal AA-NAT protein expression.

No MeSH data available.


Related in: MedlinePlus