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Gene Signature of Human Oral Mucosa Fibroblasts: Comparison with Dermal Fibroblasts and Induced Pluripotent Stem Cells.

Miyoshi K, Horiguchi T, Tanimura A, Hagita H, Noma T - Biomed Res Int (2015)

Bottom Line: As a common feature of fibroblasts, both hOFs and hDFs expressed glycolipid metabolism-related genes at higher levels compared with hOF-iPSCs.Distinct characteristics of hOFs compared with hDFs included a high expression of glycoprotein genes, involved in signaling, extracellular matrix, membrane, and receptor proteins, besides a low expression of HOX genes, the hDFs-markers.The results of the pathway analyses indicated that tissue-reconstructive, proliferative, and signaling pathways are active, whereas senescence-related genes in p53 pathway are inactive in hOFs.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.

ABSTRACT
Oral mucosa is a useful material for regeneration therapy with the advantages of its accessibility and versatility regardless of age and gender. However, little is known about the molecular characteristics of oral mucosa. Here we report the first comparative profiles of the gene signatures of human oral mucosa fibroblasts (hOFs), human dermal fibroblasts (hDFs), and hOF-derived induced pluripotent stem cells (hOF-iPSCs), linking these with biological roles by functional annotation and pathway analyses. As a common feature of fibroblasts, both hOFs and hDFs expressed glycolipid metabolism-related genes at higher levels compared with hOF-iPSCs. Distinct characteristics of hOFs compared with hDFs included a high expression of glycoprotein genes, involved in signaling, extracellular matrix, membrane, and receptor proteins, besides a low expression of HOX genes, the hDFs-markers. The results of the pathway analyses indicated that tissue-reconstructive, proliferative, and signaling pathways are active, whereas senescence-related genes in p53 pathway are inactive in hOFs. Furthermore, more than half of hOF-specific genes were similarly expressed to those of hOF-iPSC genes and might be controlled by WNT signaling. Our findings demonstrated that hOFs have unique cellular characteristics in specificity and plasticity. These data may provide useful insight into application of oral fibroblasts for direct reprograming.

No MeSH data available.


Related in: MedlinePlus

Summary of gene signatures in hOFs. (a) Overview of gene profiles in oral mucosal fibroblasts. Dotted-lined box indicates the category of genes. Red- and blue-colored words in italics show the biological function of gene categories with high and low expressions, respectively. (b) A proposed possible gene network in oral mucosal fibroblasts. Gene names in the different color are indicated as follows. Red: high expression in hOFs compared with that in hDFs (hOFs > hDFs); blue: low expression in hOFs compared with that in hDFs (hOFs < hDFs); purple: similar expression in hOFs and hDFs, but higher than hiPSCs (hOFs = hDF > hiPSCs). The box colors indicate biological characteristics or functions as follows. Pink box: possible “plastic” characteristics; green box: possible “specific” characteristics of hOFs; gray box: WNT signal regulators; lined box: biological function; dotted-lined box: the known key transcription factors. Dotted bar indicates indirect effect. Detailed explanations of (a) and (b) are described in the text.
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fig5: Summary of gene signatures in hOFs. (a) Overview of gene profiles in oral mucosal fibroblasts. Dotted-lined box indicates the category of genes. Red- and blue-colored words in italics show the biological function of gene categories with high and low expressions, respectively. (b) A proposed possible gene network in oral mucosal fibroblasts. Gene names in the different color are indicated as follows. Red: high expression in hOFs compared with that in hDFs (hOFs > hDFs); blue: low expression in hOFs compared with that in hDFs (hOFs < hDFs); purple: similar expression in hOFs and hDFs, but higher than hiPSCs (hOFs = hDF > hiPSCs). The box colors indicate biological characteristics or functions as follows. Pink box: possible “plastic” characteristics; green box: possible “specific” characteristics of hOFs; gray box: WNT signal regulators; lined box: biological function; dotted-lined box: the known key transcription factors. Dotted bar indicates indirect effect. Detailed explanations of (a) and (b) are described in the text.

Mentions: In this study, we elucidated the unique characteristics of hOFs through comparative analyses of gene expression profiles among hOFs, hDFs, and hOF-iPSCs. In Figure 5(a), we categorized the characteristic gene profile in hOFs that the common fibroblastic features as observed in hOFs and hDFs compared with hOF-iPSCs (upper box) and the specific characteristics of “hOFs” can be demonstrated by comparing with hDFs (lower box). Based on these findings, we developed the possible gene network in hOFs as shown in Figure 5(b).


Gene Signature of Human Oral Mucosa Fibroblasts: Comparison with Dermal Fibroblasts and Induced Pluripotent Stem Cells.

Miyoshi K, Horiguchi T, Tanimura A, Hagita H, Noma T - Biomed Res Int (2015)

Summary of gene signatures in hOFs. (a) Overview of gene profiles in oral mucosal fibroblasts. Dotted-lined box indicates the category of genes. Red- and blue-colored words in italics show the biological function of gene categories with high and low expressions, respectively. (b) A proposed possible gene network in oral mucosal fibroblasts. Gene names in the different color are indicated as follows. Red: high expression in hOFs compared with that in hDFs (hOFs > hDFs); blue: low expression in hOFs compared with that in hDFs (hOFs < hDFs); purple: similar expression in hOFs and hDFs, but higher than hiPSCs (hOFs = hDF > hiPSCs). The box colors indicate biological characteristics or functions as follows. Pink box: possible “plastic” characteristics; green box: possible “specific” characteristics of hOFs; gray box: WNT signal regulators; lined box: biological function; dotted-lined box: the known key transcription factors. Dotted bar indicates indirect effect. Detailed explanations of (a) and (b) are described in the text.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538314&req=5

fig5: Summary of gene signatures in hOFs. (a) Overview of gene profiles in oral mucosal fibroblasts. Dotted-lined box indicates the category of genes. Red- and blue-colored words in italics show the biological function of gene categories with high and low expressions, respectively. (b) A proposed possible gene network in oral mucosal fibroblasts. Gene names in the different color are indicated as follows. Red: high expression in hOFs compared with that in hDFs (hOFs > hDFs); blue: low expression in hOFs compared with that in hDFs (hOFs < hDFs); purple: similar expression in hOFs and hDFs, but higher than hiPSCs (hOFs = hDF > hiPSCs). The box colors indicate biological characteristics or functions as follows. Pink box: possible “plastic” characteristics; green box: possible “specific” characteristics of hOFs; gray box: WNT signal regulators; lined box: biological function; dotted-lined box: the known key transcription factors. Dotted bar indicates indirect effect. Detailed explanations of (a) and (b) are described in the text.
Mentions: In this study, we elucidated the unique characteristics of hOFs through comparative analyses of gene expression profiles among hOFs, hDFs, and hOF-iPSCs. In Figure 5(a), we categorized the characteristic gene profile in hOFs that the common fibroblastic features as observed in hOFs and hDFs compared with hOF-iPSCs (upper box) and the specific characteristics of “hOFs” can be demonstrated by comparing with hDFs (lower box). Based on these findings, we developed the possible gene network in hOFs as shown in Figure 5(b).

Bottom Line: As a common feature of fibroblasts, both hOFs and hDFs expressed glycolipid metabolism-related genes at higher levels compared with hOF-iPSCs.Distinct characteristics of hOFs compared with hDFs included a high expression of glycoprotein genes, involved in signaling, extracellular matrix, membrane, and receptor proteins, besides a low expression of HOX genes, the hDFs-markers.The results of the pathway analyses indicated that tissue-reconstructive, proliferative, and signaling pathways are active, whereas senescence-related genes in p53 pathway are inactive in hOFs.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.

ABSTRACT
Oral mucosa is a useful material for regeneration therapy with the advantages of its accessibility and versatility regardless of age and gender. However, little is known about the molecular characteristics of oral mucosa. Here we report the first comparative profiles of the gene signatures of human oral mucosa fibroblasts (hOFs), human dermal fibroblasts (hDFs), and hOF-derived induced pluripotent stem cells (hOF-iPSCs), linking these with biological roles by functional annotation and pathway analyses. As a common feature of fibroblasts, both hOFs and hDFs expressed glycolipid metabolism-related genes at higher levels compared with hOF-iPSCs. Distinct characteristics of hOFs compared with hDFs included a high expression of glycoprotein genes, involved in signaling, extracellular matrix, membrane, and receptor proteins, besides a low expression of HOX genes, the hDFs-markers. The results of the pathway analyses indicated that tissue-reconstructive, proliferative, and signaling pathways are active, whereas senescence-related genes in p53 pathway are inactive in hOFs. Furthermore, more than half of hOF-specific genes were similarly expressed to those of hOF-iPSC genes and might be controlled by WNT signaling. Our findings demonstrated that hOFs have unique cellular characteristics in specificity and plasticity. These data may provide useful insight into application of oral fibroblasts for direct reprograming.

No MeSH data available.


Related in: MedlinePlus