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Genetic Diversity of Human Adenovirus in Children with Acute Gastroenteritis, Albania, 2013-2015.

La Rosa G, Della Libera S, Petricca S, Iaconelli M, Donia D, Saccucci P, Cenko F, Xhelilaj G, Divizia M - Biomed Res Int (2015)

Bottom Line: Faecal specimens from children admitted with acute gastroenteritis to the Paediatric Hospital in Tirana were screened for HAdV, using broad-range primers targeting the hexon gene, in combination with species-specific primers targeting the fiber gene.Rotavirus coinfection was present in 9/33 (27.2%) positive samples.In the remaining 24 positive samples (12 enteric--F species; 12 nonenteric--A, B, or C species), HAdVs were detected as unique viral pathogens, suggesting that HAdV may be an important cause of diarrhoea in children requiring hospitalization.

View Article: PubMed Central - PubMed

Affiliation: Department of Environment and Primary Prevention, Istituto Superiore di Sanità, 00161 Rome, Italy.

ABSTRACT
The objectives of the present study were to assess the occurrence of human adenoviruses (HAdVs) in paediatric patients with gastroenteritis in Albania and to characterize HAdV strains. Faecal specimens from children admitted with acute gastroenteritis to the Paediatric Hospital in Tirana were screened for HAdV, using broad-range primers targeting the hexon gene, in combination with species-specific primers targeting the fiber gene. Phylogenetic analysis was then performed to assess the genetic relationships among the different sequences and between the sequences of the samples and those of the prototype strains. Adenovirus DNA was detected in 33/142 samples (23.2%); 14 belonged to species F (13 HAdV-41 and 1 HAdV-40), 13 to species C (1 HAdV-1, 8 HAdV-2, and 4 HAdV-5), 5 to species B (HAdV-3), and 1 to species A (HAdV-12). Rotavirus coinfection was present in 9/33 (27.2%) positive samples. In the remaining 24 positive samples (12 enteric--F species; 12 nonenteric--A, B, or C species), HAdVs were detected as unique viral pathogens, suggesting that HAdV may be an important cause of diarrhoea in children requiring hospitalization. This is the first study investigating the presence of human adenoviruses (species A-G) as etiologic agents of viral gastroenteritis in children in Albania.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree based on partial nucleotide sequences of the hexon region.
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Related In: Results  -  Collection


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fig1: Phylogenetic tree based on partial nucleotide sequences of the hexon region.

Mentions: The nucleotide sequences were compared to reference adenovirus strains available in the GenBank by BLAST, and molecular type identities were assigned based on the identity of the closest match. The phylogenetic tree was constructed based on the best fit model of nucleotide substitution, which was selected from 24 models available in the software, using the minimum Akaike Information Criterion (AIC). The reliability of the phylogenetic tree was determined by bootstrap resampling of 1,000 replicates. The phylogenetic tree based on nucleotide sequences of adenovirus hexon gene obtained in this study is presented in Figure 1.


Genetic Diversity of Human Adenovirus in Children with Acute Gastroenteritis, Albania, 2013-2015.

La Rosa G, Della Libera S, Petricca S, Iaconelli M, Donia D, Saccucci P, Cenko F, Xhelilaj G, Divizia M - Biomed Res Int (2015)

Phylogenetic tree based on partial nucleotide sequences of the hexon region.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4538309&req=5

fig1: Phylogenetic tree based on partial nucleotide sequences of the hexon region.
Mentions: The nucleotide sequences were compared to reference adenovirus strains available in the GenBank by BLAST, and molecular type identities were assigned based on the identity of the closest match. The phylogenetic tree was constructed based on the best fit model of nucleotide substitution, which was selected from 24 models available in the software, using the minimum Akaike Information Criterion (AIC). The reliability of the phylogenetic tree was determined by bootstrap resampling of 1,000 replicates. The phylogenetic tree based on nucleotide sequences of adenovirus hexon gene obtained in this study is presented in Figure 1.

Bottom Line: Faecal specimens from children admitted with acute gastroenteritis to the Paediatric Hospital in Tirana were screened for HAdV, using broad-range primers targeting the hexon gene, in combination with species-specific primers targeting the fiber gene.Rotavirus coinfection was present in 9/33 (27.2%) positive samples.In the remaining 24 positive samples (12 enteric--F species; 12 nonenteric--A, B, or C species), HAdVs were detected as unique viral pathogens, suggesting that HAdV may be an important cause of diarrhoea in children requiring hospitalization.

View Article: PubMed Central - PubMed

Affiliation: Department of Environment and Primary Prevention, Istituto Superiore di Sanità, 00161 Rome, Italy.

ABSTRACT
The objectives of the present study were to assess the occurrence of human adenoviruses (HAdVs) in paediatric patients with gastroenteritis in Albania and to characterize HAdV strains. Faecal specimens from children admitted with acute gastroenteritis to the Paediatric Hospital in Tirana were screened for HAdV, using broad-range primers targeting the hexon gene, in combination with species-specific primers targeting the fiber gene. Phylogenetic analysis was then performed to assess the genetic relationships among the different sequences and between the sequences of the samples and those of the prototype strains. Adenovirus DNA was detected in 33/142 samples (23.2%); 14 belonged to species F (13 HAdV-41 and 1 HAdV-40), 13 to species C (1 HAdV-1, 8 HAdV-2, and 4 HAdV-5), 5 to species B (HAdV-3), and 1 to species A (HAdV-12). Rotavirus coinfection was present in 9/33 (27.2%) positive samples. In the remaining 24 positive samples (12 enteric--F species; 12 nonenteric--A, B, or C species), HAdVs were detected as unique viral pathogens, suggesting that HAdV may be an important cause of diarrhoea in children requiring hospitalization. This is the first study investigating the presence of human adenoviruses (species A-G) as etiologic agents of viral gastroenteritis in children in Albania.

No MeSH data available.


Related in: MedlinePlus