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Blood Monocytes and Their Subsets: Established Features and Open Questions.

Ziegler-Heitbrock L - Front Immunol (2015)

Bottom Line: In contrast to the past reliance on morphology, the identification and enumeration of blood monocytes are nowadays done with monoclonal antibodies and flow cytometry and this allows for subdivision into classical, intermediate, and non-classical monocytes.Using specific cell surface markers, dendritic cells in blood can be segregated from these monocytes.While in the past, changes in monocyte numbers as determined in standard hematology counters have not had any relevant clinical impact, the subset analysis now has uncovered informative changes that may be used in management of disease.

View Article: PubMed Central - PubMed

Affiliation: Helmholtz Zentrum München, Asklepios Fachkliniken München-Gauting , Gauting , Germany.

ABSTRACT
In contrast to the past reliance on morphology, the identification and enumeration of blood monocytes are nowadays done with monoclonal antibodies and flow cytometry and this allows for subdivision into classical, intermediate, and non-classical monocytes. Using specific cell surface markers, dendritic cells in blood can be segregated from these monocytes. While in the past, changes in monocyte numbers as determined in standard hematology counters have not had any relevant clinical impact, the subset analysis now has uncovered informative changes that may be used in management of disease.

No MeSH data available.


Blood monocyte subsets in man. Illustration of the definition of human monocyte subsets in health based on a typical distribution of events in a CD14 CD16 staining.
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Figure 1: Blood monocyte subsets in man. Illustration of the definition of human monocyte subsets in health based on a typical distribution of events in a CD14 CD16 staining.

Mentions: In 2010, an international consortium under the auspices of the IUIS and the WHO has proposed a nomenclature for monocyte subpopulations (38). The proposal defined the major population of CD14high cells found in human blood as classical monocytes and the minor population of cells with low CD14 and high CD16 as non-classical monocytes. A population in between these two subsets was termed intermediate monocytes (see Figure 1).


Blood Monocytes and Their Subsets: Established Features and Open Questions.

Ziegler-Heitbrock L - Front Immunol (2015)

Blood monocyte subsets in man. Illustration of the definition of human monocyte subsets in health based on a typical distribution of events in a CD14 CD16 staining.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4538304&req=5

Figure 1: Blood monocyte subsets in man. Illustration of the definition of human monocyte subsets in health based on a typical distribution of events in a CD14 CD16 staining.
Mentions: In 2010, an international consortium under the auspices of the IUIS and the WHO has proposed a nomenclature for monocyte subpopulations (38). The proposal defined the major population of CD14high cells found in human blood as classical monocytes and the minor population of cells with low CD14 and high CD16 as non-classical monocytes. A population in between these two subsets was termed intermediate monocytes (see Figure 1).

Bottom Line: In contrast to the past reliance on morphology, the identification and enumeration of blood monocytes are nowadays done with monoclonal antibodies and flow cytometry and this allows for subdivision into classical, intermediate, and non-classical monocytes.Using specific cell surface markers, dendritic cells in blood can be segregated from these monocytes.While in the past, changes in monocyte numbers as determined in standard hematology counters have not had any relevant clinical impact, the subset analysis now has uncovered informative changes that may be used in management of disease.

View Article: PubMed Central - PubMed

Affiliation: Helmholtz Zentrum München, Asklepios Fachkliniken München-Gauting , Gauting , Germany.

ABSTRACT
In contrast to the past reliance on morphology, the identification and enumeration of blood monocytes are nowadays done with monoclonal antibodies and flow cytometry and this allows for subdivision into classical, intermediate, and non-classical monocytes. Using specific cell surface markers, dendritic cells in blood can be segregated from these monocytes. While in the past, changes in monocyte numbers as determined in standard hematology counters have not had any relevant clinical impact, the subset analysis now has uncovered informative changes that may be used in management of disease.

No MeSH data available.