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Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction.

Zhao X, Wu J, Gray CD, McGregor K, Rossi AG, Morrison H, Jansen MA, Gray GA - Am. J. Physiol. Heart Circ. Physiol. (2015)

Bottom Line: Experimental studies therefore commonly assess injury by histological analysis of sections sampled from the infarcted heart, an approach that is labor intensive, can be subjective, and does not fully assess the extent of injury.Tissue processing for OPT did not compromise subsequent immunohistochemical detection of endothelial cell and inflammatory cell markers.OPT is thus a nondestructive, efficient, and accurate approach for routine in vitro assessment of murine myocardial infarct volume.

View Article: PubMed Central - PubMed

Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;

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Immunohistochemical staining of heart sections are not compromised by prior tissue preparation for OPT scanning. Sections, collected from paraffin-embedded hearts of 7 days postinfarct mice, were immunostained for detection of macrophages (A, Mac-2) and endothelial cells (B, CD31). Scale bars are 500 μm in left panels and 50 μm in right panels.
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Figure 7: Immunohistochemical staining of heart sections are not compromised by prior tissue preparation for OPT scanning. Sections, collected from paraffin-embedded hearts of 7 days postinfarct mice, were immunostained for detection of macrophages (A, Mac-2) and endothelial cells (B, CD31). Scale bars are 500 μm in left panels and 50 μm in right panels.

Mentions: In hearts previously subjected to OPT scanning, immunohistochemical staining using antibodies directed against markers of macrophages (Mac-2) and endothelial cells (CD31) occurred with the expected intensity and distribution (Fig. 7).


Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction.

Zhao X, Wu J, Gray CD, McGregor K, Rossi AG, Morrison H, Jansen MA, Gray GA - Am. J. Physiol. Heart Circ. Physiol. (2015)

Immunohistochemical staining of heart sections are not compromised by prior tissue preparation for OPT scanning. Sections, collected from paraffin-embedded hearts of 7 days postinfarct mice, were immunostained for detection of macrophages (A, Mac-2) and endothelial cells (B, CD31). Scale bars are 500 μm in left panels and 50 μm in right panels.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4537945&req=5

Figure 7: Immunohistochemical staining of heart sections are not compromised by prior tissue preparation for OPT scanning. Sections, collected from paraffin-embedded hearts of 7 days postinfarct mice, were immunostained for detection of macrophages (A, Mac-2) and endothelial cells (B, CD31). Scale bars are 500 μm in left panels and 50 μm in right panels.
Mentions: In hearts previously subjected to OPT scanning, immunohistochemical staining using antibodies directed against markers of macrophages (Mac-2) and endothelial cells (CD31) occurred with the expected intensity and distribution (Fig. 7).

Bottom Line: Experimental studies therefore commonly assess injury by histological analysis of sections sampled from the infarcted heart, an approach that is labor intensive, can be subjective, and does not fully assess the extent of injury.Tissue processing for OPT did not compromise subsequent immunohistochemical detection of endothelial cell and inflammatory cell markers.OPT is thus a nondestructive, efficient, and accurate approach for routine in vitro assessment of murine myocardial infarct volume.

View Article: PubMed Central - PubMed

Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;

Show MeSH
Related in: MedlinePlus