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Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction.

Zhao X, Wu J, Gray CD, McGregor K, Rossi AG, Morrison H, Jansen MA, Gray GA - Am. J. Physiol. Heart Circ. Physiol. (2015)

Bottom Line: Experimental studies therefore commonly assess injury by histological analysis of sections sampled from the infarcted heart, an approach that is labor intensive, can be subjective, and does not fully assess the extent of injury.Tissue processing for OPT did not compromise subsequent immunohistochemical detection of endothelial cell and inflammatory cell markers.OPT is thus a nondestructive, efficient, and accurate approach for routine in vitro assessment of murine myocardial infarct volume.

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Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;

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Correlation of systolic function with infarct volume assessed by 3D OPT and LGE-MRI. Left ventricular ejection fraction (LVEF) was calculated as an index of systolic function by cine-MRI. Infarct volume generated from 3D OPT (A) and LGE-MRI (B) are both highly correlated with LVEF, n = 9.
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Figure 6: Correlation of systolic function with infarct volume assessed by 3D OPT and LGE-MRI. Left ventricular ejection fraction (LVEF) was calculated as an index of systolic function by cine-MRI. Infarct volume generated from 3D OPT (A) and LGE-MRI (B) are both highly correlated with LVEF, n = 9.

Mentions: Left ventricular ejection fraction (LVEF) was measured by cine-MRI at the time of LGE-MRI assessment as an index of systolic function. Infarct volume derived from OPT was found to correlate highly with function (R2 = 0.7684, P = 0.0019, Fig. 6A) and to a similar extent as correlation of infarct volume generated from LGE-MRI and LVEF (R2 = 0.7794, P = 0.0016, Fig. 6B).


Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction.

Zhao X, Wu J, Gray CD, McGregor K, Rossi AG, Morrison H, Jansen MA, Gray GA - Am. J. Physiol. Heart Circ. Physiol. (2015)

Correlation of systolic function with infarct volume assessed by 3D OPT and LGE-MRI. Left ventricular ejection fraction (LVEF) was calculated as an index of systolic function by cine-MRI. Infarct volume generated from 3D OPT (A) and LGE-MRI (B) are both highly correlated with LVEF, n = 9.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4537945&req=5

Figure 6: Correlation of systolic function with infarct volume assessed by 3D OPT and LGE-MRI. Left ventricular ejection fraction (LVEF) was calculated as an index of systolic function by cine-MRI. Infarct volume generated from 3D OPT (A) and LGE-MRI (B) are both highly correlated with LVEF, n = 9.
Mentions: Left ventricular ejection fraction (LVEF) was measured by cine-MRI at the time of LGE-MRI assessment as an index of systolic function. Infarct volume derived from OPT was found to correlate highly with function (R2 = 0.7684, P = 0.0019, Fig. 6A) and to a similar extent as correlation of infarct volume generated from LGE-MRI and LVEF (R2 = 0.7794, P = 0.0016, Fig. 6B).

Bottom Line: Experimental studies therefore commonly assess injury by histological analysis of sections sampled from the infarcted heart, an approach that is labor intensive, can be subjective, and does not fully assess the extent of injury.Tissue processing for OPT did not compromise subsequent immunohistochemical detection of endothelial cell and inflammatory cell markers.OPT is thus a nondestructive, efficient, and accurate approach for routine in vitro assessment of murine myocardial infarct volume.

View Article: PubMed Central - PubMed

Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;

Show MeSH
Related in: MedlinePlus