Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction.
Bottom Line: Experimental studies therefore commonly assess injury by histological analysis of sections sampled from the infarcted heart, an approach that is labor intensive, can be subjective, and does not fully assess the extent of injury.Tissue processing for OPT did not compromise subsequent immunohistochemical detection of endothelial cell and inflammatory cell markers.OPT is thus a nondestructive, efficient, and accurate approach for routine in vitro assessment of murine myocardial infarct volume.
Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;Show MeSH
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Mentions: Left ventricular ejection fraction (LVEF) was measured by cine-MRI at the time of LGE-MRI assessment as an index of systolic function. Infarct volume derived from OPT was found to correlate highly with function (R2 = 0.7684, P = 0.0019, Fig. 6A) and to a similar extent as correlation of infarct volume generated from LGE-MRI and LVEF (R2 = 0.7794, P = 0.0016, Fig. 6B).
Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;