Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction.
Bottom Line: Experimental studies therefore commonly assess injury by histological analysis of sections sampled from the infarcted heart, an approach that is labor intensive, can be subjective, and does not fully assess the extent of injury.Intact, perfusion-fixed, optically cleared hearts, collected from mice 7 days after induction of MI by coronary artery occlusion, were scanned by a tomograph for autofluorescence emission after UV excitation, generating >400 transaxial sections for reconstruction.Tissue processing for OPT did not compromise subsequent immunohistochemical detection of endothelial cell and inflammatory cell markers.
Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;Show MeSH
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Mentions: Noninvasive cardiac LGE-MRI was performed in mice 7 days after induction of MI and immediately prior to collection of hearts for OPT imaging. Four-chamber images were generated from both techniques (Fig. 5, A and D), and 2D sections collected from both indicated matching localization and extent of injury (Fig. 5, B and C). The average infarct volume was 29.3 ± 3.1% LV when assessed by LGE-MRI and 23.3 ± 3.0% LV when assessed by OPT (P = 0.93, n = 9). Correlation analysis shows that the two methods correlated strongly for infarct volume measurement (R2 = 0.76, P = 0.002, n = 9, Fig. 5E). Bland-Altman analysis indicates no obvious systematic bias in measurements of infarct volume (6.01% LV, n = 9), and all plots were within the limits of agreement (± 1.96 SD, −3.19 to 15.21% LV, Fig. 5F).
Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;