Limits...
Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction.

Zhao X, Wu J, Gray CD, McGregor K, Rossi AG, Morrison H, Jansen MA, Gray GA - Am. J. Physiol. Heart Circ. Physiol. (2015)

Bottom Line: Experimental studies therefore commonly assess injury by histological analysis of sections sampled from the infarcted heart, an approach that is labor intensive, can be subjective, and does not fully assess the extent of injury.Tissue processing for OPT did not compromise subsequent immunohistochemical detection of endothelial cell and inflammatory cell markers.OPT is thus a nondestructive, efficient, and accurate approach for routine in vitro assessment of murine myocardial infarct volume.

View Article: PubMed Central - PubMed

Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;

Show MeSH

Related in: MedlinePlus

Comparison of LGE-MRI and 3D OPT for infarct detection and volume assessment. A longitudinal 4-chamber image obtained by cine-MRI (A) was used to position collection of short-axis sections imaged by LGE-MRI (B, white region indicates infarction while viable myocardium in black) or OPT reconstructions (C, infarct dark and viable myocardium bright). D: the OPT nontomographic projection of the same heart. Correlation (E) and Bland-Altman analysis (F) were performed to compare the 2 methods (n = 9 mice).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4537945&req=5

Figure 5: Comparison of LGE-MRI and 3D OPT for infarct detection and volume assessment. A longitudinal 4-chamber image obtained by cine-MRI (A) was used to position collection of short-axis sections imaged by LGE-MRI (B, white region indicates infarction while viable myocardium in black) or OPT reconstructions (C, infarct dark and viable myocardium bright). D: the OPT nontomographic projection of the same heart. Correlation (E) and Bland-Altman analysis (F) were performed to compare the 2 methods (n = 9 mice).

Mentions: Noninvasive cardiac LGE-MRI was performed in mice 7 days after induction of MI and immediately prior to collection of hearts for OPT imaging. Four-chamber images were generated from both techniques (Fig. 5, A and D), and 2D sections collected from both indicated matching localization and extent of injury (Fig. 5, B and C). The average infarct volume was 29.3 ± 3.1% LV when assessed by LGE-MRI and 23.3 ± 3.0% LV when assessed by OPT (P = 0.93, n = 9). Correlation analysis shows that the two methods correlated strongly for infarct volume measurement (R2 = 0.76, P = 0.002, n = 9, Fig. 5E). Bland-Altman analysis indicates no obvious systematic bias in measurements of infarct volume (6.01% LV, n = 9), and all plots were within the limits of agreement (± 1.96 SD, −3.19 to 15.21% LV, Fig. 5F).


Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction.

Zhao X, Wu J, Gray CD, McGregor K, Rossi AG, Morrison H, Jansen MA, Gray GA - Am. J. Physiol. Heart Circ. Physiol. (2015)

Comparison of LGE-MRI and 3D OPT for infarct detection and volume assessment. A longitudinal 4-chamber image obtained by cine-MRI (A) was used to position collection of short-axis sections imaged by LGE-MRI (B, white region indicates infarction while viable myocardium in black) or OPT reconstructions (C, infarct dark and viable myocardium bright). D: the OPT nontomographic projection of the same heart. Correlation (E) and Bland-Altman analysis (F) were performed to compare the 2 methods (n = 9 mice).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4537945&req=5

Figure 5: Comparison of LGE-MRI and 3D OPT for infarct detection and volume assessment. A longitudinal 4-chamber image obtained by cine-MRI (A) was used to position collection of short-axis sections imaged by LGE-MRI (B, white region indicates infarction while viable myocardium in black) or OPT reconstructions (C, infarct dark and viable myocardium bright). D: the OPT nontomographic projection of the same heart. Correlation (E) and Bland-Altman analysis (F) were performed to compare the 2 methods (n = 9 mice).
Mentions: Noninvasive cardiac LGE-MRI was performed in mice 7 days after induction of MI and immediately prior to collection of hearts for OPT imaging. Four-chamber images were generated from both techniques (Fig. 5, A and D), and 2D sections collected from both indicated matching localization and extent of injury (Fig. 5, B and C). The average infarct volume was 29.3 ± 3.1% LV when assessed by LGE-MRI and 23.3 ± 3.0% LV when assessed by OPT (P = 0.93, n = 9). Correlation analysis shows that the two methods correlated strongly for infarct volume measurement (R2 = 0.76, P = 0.002, n = 9, Fig. 5E). Bland-Altman analysis indicates no obvious systematic bias in measurements of infarct volume (6.01% LV, n = 9), and all plots were within the limits of agreement (± 1.96 SD, −3.19 to 15.21% LV, Fig. 5F).

Bottom Line: Experimental studies therefore commonly assess injury by histological analysis of sections sampled from the infarcted heart, an approach that is labor intensive, can be subjective, and does not fully assess the extent of injury.Tissue processing for OPT did not compromise subsequent immunohistochemical detection of endothelial cell and inflammatory cell markers.OPT is thus a nondestructive, efficient, and accurate approach for routine in vitro assessment of murine myocardial infarct volume.

View Article: PubMed Central - PubMed

Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;

Show MeSH
Related in: MedlinePlus