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Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction.

Zhao X, Wu J, Gray CD, McGregor K, Rossi AG, Morrison H, Jansen MA, Gray GA - Am. J. Physiol. Heart Circ. Physiol. (2015)

Bottom Line: Experimental studies therefore commonly assess injury by histological analysis of sections sampled from the infarcted heart, an approach that is labor intensive, can be subjective, and does not fully assess the extent of injury.Tissue processing for OPT did not compromise subsequent immunohistochemical detection of endothelial cell and inflammatory cell markers.OPT is thus a nondestructive, efficient, and accurate approach for routine in vitro assessment of murine myocardial infarct volume.

View Article: PubMed Central - PubMed

Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;

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Comparison of infarct size determined by OPT and histology in 2D sections. Short-axis sections from the same heart (A) are shown from the level of ligation (1) to the heart apex (8), viewed both as OPT tomographic reconstructions (bottom panels) and corresponding Masson's trichrome-stained histological sections (top panels). Viable myocardium is bright (OPT) or red/pink (histology), while infarcted tissue is dark (OPT) or blue (histology). Correlation (B) and Bland-Altman analysis (C) were performed to compare the 2 methods on infarct size measurement. Sections 3–6 were used for 2D determination of infarct size (highlighted by red rectangles).
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Figure 4: Comparison of infarct size determined by OPT and histology in 2D sections. Short-axis sections from the same heart (A) are shown from the level of ligation (1) to the heart apex (8), viewed both as OPT tomographic reconstructions (bottom panels) and corresponding Masson's trichrome-stained histological sections (top panels). Viable myocardium is bright (OPT) or red/pink (histology), while infarcted tissue is dark (OPT) or blue (histology). Correlation (B) and Bland-Altman analysis (C) were performed to compare the 2 methods on infarct size measurement. Sections 3–6 were used for 2D determination of infarct size (highlighted by red rectangles).

Mentions: 2D OPT reconstruction provided remarkably similar identification of infarct injury to Masson's trichrome-stained histological sections subsequently collected at corresponding levels of the same heart (Fig. 4A). A wide range of infarct sizes were acquired (Fig. 4B), and significant correlation was evident between infarct sizes derived from the average of four histological sections sampled from the infarct and four optical OPT slices (r2 = 0.99, P < 0.0001, n = 8). Bland-Altman analysis indicated a bias of only 0.046% LV, and all plots were within the ±1.96 SD limits of agreement (−3.75 to 3.84% LV) (Fig. 4C).


Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction.

Zhao X, Wu J, Gray CD, McGregor K, Rossi AG, Morrison H, Jansen MA, Gray GA - Am. J. Physiol. Heart Circ. Physiol. (2015)

Comparison of infarct size determined by OPT and histology in 2D sections. Short-axis sections from the same heart (A) are shown from the level of ligation (1) to the heart apex (8), viewed both as OPT tomographic reconstructions (bottom panels) and corresponding Masson's trichrome-stained histological sections (top panels). Viable myocardium is bright (OPT) or red/pink (histology), while infarcted tissue is dark (OPT) or blue (histology). Correlation (B) and Bland-Altman analysis (C) were performed to compare the 2 methods on infarct size measurement. Sections 3–6 were used for 2D determination of infarct size (highlighted by red rectangles).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4537945&req=5

Figure 4: Comparison of infarct size determined by OPT and histology in 2D sections. Short-axis sections from the same heart (A) are shown from the level of ligation (1) to the heart apex (8), viewed both as OPT tomographic reconstructions (bottom panels) and corresponding Masson's trichrome-stained histological sections (top panels). Viable myocardium is bright (OPT) or red/pink (histology), while infarcted tissue is dark (OPT) or blue (histology). Correlation (B) and Bland-Altman analysis (C) were performed to compare the 2 methods on infarct size measurement. Sections 3–6 were used for 2D determination of infarct size (highlighted by red rectangles).
Mentions: 2D OPT reconstruction provided remarkably similar identification of infarct injury to Masson's trichrome-stained histological sections subsequently collected at corresponding levels of the same heart (Fig. 4A). A wide range of infarct sizes were acquired (Fig. 4B), and significant correlation was evident between infarct sizes derived from the average of four histological sections sampled from the infarct and four optical OPT slices (r2 = 0.99, P < 0.0001, n = 8). Bland-Altman analysis indicated a bias of only 0.046% LV, and all plots were within the ±1.96 SD limits of agreement (−3.75 to 3.84% LV) (Fig. 4C).

Bottom Line: Experimental studies therefore commonly assess injury by histological analysis of sections sampled from the infarcted heart, an approach that is labor intensive, can be subjective, and does not fully assess the extent of injury.Tissue processing for OPT did not compromise subsequent immunohistochemical detection of endothelial cell and inflammatory cell markers.OPT is thus a nondestructive, efficient, and accurate approach for routine in vitro assessment of murine myocardial infarct volume.

View Article: PubMed Central - PubMed

Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;

Show MeSH
Related in: MedlinePlus