Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction.
Bottom Line: Experimental studies therefore commonly assess injury by histological analysis of sections sampled from the infarcted heart, an approach that is labor intensive, can be subjective, and does not fully assess the extent of injury.Intact, perfusion-fixed, optically cleared hearts, collected from mice 7 days after induction of MI by coronary artery occlusion, were scanned by a tomograph for autofluorescence emission after UV excitation, generating >400 transaxial sections for reconstruction.Tissue processing for OPT did not compromise subsequent immunohistochemical detection of endothelial cell and inflammatory cell markers.
Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;Show MeSH
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Mentions: 2D OPT reconstruction provided remarkably similar identification of infarct injury to Masson's trichrome-stained histological sections subsequently collected at corresponding levels of the same heart (Fig. 4A). A wide range of infarct sizes were acquired (Fig. 4B), and significant correlation was evident between infarct sizes derived from the average of four histological sections sampled from the infarct and four optical OPT slices (r2 = 0.99, P < 0.0001, n = 8). Bland-Altman analysis indicated a bias of only 0.046% LV, and all plots were within the ±1.96 SD limits of agreement (−3.75 to 3.84% LV) (Fig. 4C).
Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;