Optical projection tomography permits efficient assessment of infarct volume in the murine heart postmyocardial infarction.
Bottom Line: Experimental studies therefore commonly assess injury by histological analysis of sections sampled from the infarcted heart, an approach that is labor intensive, can be subjective, and does not fully assess the extent of injury.Intact, perfusion-fixed, optically cleared hearts, collected from mice 7 days after induction of MI by coronary artery occlusion, were scanned by a tomograph for autofluorescence emission after UV excitation, generating >400 transaxial sections for reconstruction.Tissue processing for OPT did not compromise subsequent immunohistochemical detection of endothelial cell and inflammatory cell markers.
Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;Show MeSH
Related in: MedlinePlus
Mentions: We obtained 10 male C57BL/6 mice, age 12–14 wk, from Harlan UK. All animal experiments were performed in accordance with the Animals (Scientific Procedures) Act (UK), 1986 and approved by the University of Edinburgh Preclinical Ethical Review Committee. All mice underwent the protocol described in Fig. 1.
Affiliation: BHF/University Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom;