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Decreased Activity in Neuropathic Pain Form and Gene Expression of Cyclin-Dependent Kinase5 and Glycogen Synthase Kinase-3 Beta in Soleus Muscle of Wistar Male Rats.

Rahmati M, Taherabadi SJ, Mehrabi M - Iran Red Crescent Med J (2015)

Bottom Line: Following 4 weeks after surgery, the left soleus muscle was removed and mRNA levels were determined by real-time Polymerase Chain Reaction (PCR).Soleus muscle weight as well as CDK5 mRNA levels (less than ~ 0.4 fold) was decreased and GSK-3β mRNA levels (up to ~ 7 folds) increased in L5 ligated animals.These results showed enhanced muscle atrophy processes following peripheral nerve damage and might provide a useful approach to study underlying muscle mechanisms associated with clinical neuropathic pain syndromes.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Education and Sport Sciences, Lorestan University, Khoram Abad, IR Iran.

ABSTRACT

Background: The relationship between decreased activity/neuropathic pain and gene expression alterations in soleus muscle has remained elusive.

Objectives: In this experimental study, we investigated the effects of decreased activity in neuropathic pain form on Cyclin-Dependent Kinase 5 (CDK5) and Glycogen Synthase Kinase-3 β (GSK-3β) gene expression in soleus muscle of rats.

Materials and methods: Twelve male Wistar rats were randomly divided into three groups: (1) tight ligation of the L5 spinal nerve (SNL: n = 4); (2) sham surgery (Sham: n = 4), and (3) control (C: n = 4). The threshold to produce a withdrawal response to a mechanical and thermal stimulus was measured using von Frey filaments and radiation heat apparatus, respectively. Following 4 weeks after surgery, the left soleus muscle was removed and mRNA levels were determined by real-time Polymerase Chain Reaction (PCR).

Results: Compared to control animals, L5 ligated animals developed mechanical and heat hypersensitivity during total period of study. Soleus muscle weight as well as CDK5 mRNA levels (less than ~ 0.4 fold) was decreased and GSK-3β mRNA levels (up to ~ 7 folds) increased in L5 ligated animals.

Conclusions: These results showed enhanced muscle atrophy processes following peripheral nerve damage and might provide a useful approach to study underlying muscle mechanisms associated with clinical neuropathic pain syndromes.

No MeSH data available.


Related in: MedlinePlus

Real-Time Amplification of CDK5 mRNA in Soleus MuscleData are presented as mean ± SD, which indicate significant differences with other groups (P < 0.05).
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fig20150: Real-Time Amplification of CDK5 mRNA in Soleus MuscleData are presented as mean ± SD, which indicate significant differences with other groups (P < 0.05).

Mentions: Weight of the soleus muscle decreased in SNL rats and this result indicates muscular atrophy as a consequence of tight ligation of the L5 spinal nerve (Figure 3). To elucidate the possible regulation of CDK5 and GSK-3β at mRNA level in rat models with neuropathic pain, we examined CDK5 and GSK-3β at mRNA level in soleus muscle after 4 weeks of tight ligation of L5 spinal nerves. Agarose gel electrophoresis presented a single band with the expected size (Figure 4) and as indicated in Figure 5 and 6CDK5 mRNA levels were down regulated and GSK-3β mRNA levels were up regulated.


Decreased Activity in Neuropathic Pain Form and Gene Expression of Cyclin-Dependent Kinase5 and Glycogen Synthase Kinase-3 Beta in Soleus Muscle of Wistar Male Rats.

Rahmati M, Taherabadi SJ, Mehrabi M - Iran Red Crescent Med J (2015)

Real-Time Amplification of CDK5 mRNA in Soleus MuscleData are presented as mean ± SD, which indicate significant differences with other groups (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4537785&req=5

fig20150: Real-Time Amplification of CDK5 mRNA in Soleus MuscleData are presented as mean ± SD, which indicate significant differences with other groups (P < 0.05).
Mentions: Weight of the soleus muscle decreased in SNL rats and this result indicates muscular atrophy as a consequence of tight ligation of the L5 spinal nerve (Figure 3). To elucidate the possible regulation of CDK5 and GSK-3β at mRNA level in rat models with neuropathic pain, we examined CDK5 and GSK-3β at mRNA level in soleus muscle after 4 weeks of tight ligation of L5 spinal nerves. Agarose gel electrophoresis presented a single band with the expected size (Figure 4) and as indicated in Figure 5 and 6CDK5 mRNA levels were down regulated and GSK-3β mRNA levels were up regulated.

Bottom Line: Following 4 weeks after surgery, the left soleus muscle was removed and mRNA levels were determined by real-time Polymerase Chain Reaction (PCR).Soleus muscle weight as well as CDK5 mRNA levels (less than ~ 0.4 fold) was decreased and GSK-3β mRNA levels (up to ~ 7 folds) increased in L5 ligated animals.These results showed enhanced muscle atrophy processes following peripheral nerve damage and might provide a useful approach to study underlying muscle mechanisms associated with clinical neuropathic pain syndromes.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Education and Sport Sciences, Lorestan University, Khoram Abad, IR Iran.

ABSTRACT

Background: The relationship between decreased activity/neuropathic pain and gene expression alterations in soleus muscle has remained elusive.

Objectives: In this experimental study, we investigated the effects of decreased activity in neuropathic pain form on Cyclin-Dependent Kinase 5 (CDK5) and Glycogen Synthase Kinase-3 β (GSK-3β) gene expression in soleus muscle of rats.

Materials and methods: Twelve male Wistar rats were randomly divided into three groups: (1) tight ligation of the L5 spinal nerve (SNL: n = 4); (2) sham surgery (Sham: n = 4), and (3) control (C: n = 4). The threshold to produce a withdrawal response to a mechanical and thermal stimulus was measured using von Frey filaments and radiation heat apparatus, respectively. Following 4 weeks after surgery, the left soleus muscle was removed and mRNA levels were determined by real-time Polymerase Chain Reaction (PCR).

Results: Compared to control animals, L5 ligated animals developed mechanical and heat hypersensitivity during total period of study. Soleus muscle weight as well as CDK5 mRNA levels (less than ~ 0.4 fold) was decreased and GSK-3β mRNA levels (up to ~ 7 folds) increased in L5 ligated animals.

Conclusions: These results showed enhanced muscle atrophy processes following peripheral nerve damage and might provide a useful approach to study underlying muscle mechanisms associated with clinical neuropathic pain syndromes.

No MeSH data available.


Related in: MedlinePlus