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Decreased Activity in Neuropathic Pain Form and Gene Expression of Cyclin-Dependent Kinase5 and Glycogen Synthase Kinase-3 Beta in Soleus Muscle of Wistar Male Rats.

Rahmati M, Taherabadi SJ, Mehrabi M - Iran Red Crescent Med J (2015)

Bottom Line: Following 4 weeks after surgery, the left soleus muscle was removed and mRNA levels were determined by real-time Polymerase Chain Reaction (PCR).Soleus muscle weight as well as CDK5 mRNA levels (less than ~ 0.4 fold) was decreased and GSK-3β mRNA levels (up to ~ 7 folds) increased in L5 ligated animals.These results showed enhanced muscle atrophy processes following peripheral nerve damage and might provide a useful approach to study underlying muscle mechanisms associated with clinical neuropathic pain syndromes.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Education and Sport Sciences, Lorestan University, Khoram Abad, IR Iran.

ABSTRACT

Background: The relationship between decreased activity/neuropathic pain and gene expression alterations in soleus muscle has remained elusive.

Objectives: In this experimental study, we investigated the effects of decreased activity in neuropathic pain form on Cyclin-Dependent Kinase 5 (CDK5) and Glycogen Synthase Kinase-3 β (GSK-3β) gene expression in soleus muscle of rats.

Materials and methods: Twelve male Wistar rats were randomly divided into three groups: (1) tight ligation of the L5 spinal nerve (SNL: n = 4); (2) sham surgery (Sham: n = 4), and (3) control (C: n = 4). The threshold to produce a withdrawal response to a mechanical and thermal stimulus was measured using von Frey filaments and radiation heat apparatus, respectively. Following 4 weeks after surgery, the left soleus muscle was removed and mRNA levels were determined by real-time Polymerase Chain Reaction (PCR).

Results: Compared to control animals, L5 ligated animals developed mechanical and heat hypersensitivity during total period of study. Soleus muscle weight as well as CDK5 mRNA levels (less than ~ 0.4 fold) was decreased and GSK-3β mRNA levels (up to ~ 7 folds) increased in L5 ligated animals.

Conclusions: These results showed enhanced muscle atrophy processes following peripheral nerve damage and might provide a useful approach to study underlying muscle mechanisms associated with clinical neuropathic pain syndromes.

No MeSH data available.


Related in: MedlinePlus

Thermal Withdrawal Latency of the Left Hind PawsPWT decreased in the SNL rats postoperatively on the first, second, third, and fourth weeks (P < 0.05 versus sham group). Data are presented as the mean ± SD.
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fig20147: Thermal Withdrawal Latency of the Left Hind PawsPWT decreased in the SNL rats postoperatively on the first, second, third, and fourth weeks (P < 0.05 versus sham group). Data are presented as the mean ± SD.

Mentions: All rats that received L5 SNL developed mechanical and heat hypersensitivity on the ipsilateral hind paw. As shown in Figure 1 and 2, PWT as well as PWL in the SNL group were lower postoperatively on the first, second, third and fourth weeks (P < 0.05 versus sham group), indicating that mechanical allodynia and thermal hyperalgesia has been induced by SNL operation.


Decreased Activity in Neuropathic Pain Form and Gene Expression of Cyclin-Dependent Kinase5 and Glycogen Synthase Kinase-3 Beta in Soleus Muscle of Wistar Male Rats.

Rahmati M, Taherabadi SJ, Mehrabi M - Iran Red Crescent Med J (2015)

Thermal Withdrawal Latency of the Left Hind PawsPWT decreased in the SNL rats postoperatively on the first, second, third, and fourth weeks (P < 0.05 versus sham group). Data are presented as the mean ± SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4537785&req=5

fig20147: Thermal Withdrawal Latency of the Left Hind PawsPWT decreased in the SNL rats postoperatively on the first, second, third, and fourth weeks (P < 0.05 versus sham group). Data are presented as the mean ± SD.
Mentions: All rats that received L5 SNL developed mechanical and heat hypersensitivity on the ipsilateral hind paw. As shown in Figure 1 and 2, PWT as well as PWL in the SNL group were lower postoperatively on the first, second, third and fourth weeks (P < 0.05 versus sham group), indicating that mechanical allodynia and thermal hyperalgesia has been induced by SNL operation.

Bottom Line: Following 4 weeks after surgery, the left soleus muscle was removed and mRNA levels were determined by real-time Polymerase Chain Reaction (PCR).Soleus muscle weight as well as CDK5 mRNA levels (less than ~ 0.4 fold) was decreased and GSK-3β mRNA levels (up to ~ 7 folds) increased in L5 ligated animals.These results showed enhanced muscle atrophy processes following peripheral nerve damage and might provide a useful approach to study underlying muscle mechanisms associated with clinical neuropathic pain syndromes.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Education and Sport Sciences, Lorestan University, Khoram Abad, IR Iran.

ABSTRACT

Background: The relationship between decreased activity/neuropathic pain and gene expression alterations in soleus muscle has remained elusive.

Objectives: In this experimental study, we investigated the effects of decreased activity in neuropathic pain form on Cyclin-Dependent Kinase 5 (CDK5) and Glycogen Synthase Kinase-3 β (GSK-3β) gene expression in soleus muscle of rats.

Materials and methods: Twelve male Wistar rats were randomly divided into three groups: (1) tight ligation of the L5 spinal nerve (SNL: n = 4); (2) sham surgery (Sham: n = 4), and (3) control (C: n = 4). The threshold to produce a withdrawal response to a mechanical and thermal stimulus was measured using von Frey filaments and radiation heat apparatus, respectively. Following 4 weeks after surgery, the left soleus muscle was removed and mRNA levels were determined by real-time Polymerase Chain Reaction (PCR).

Results: Compared to control animals, L5 ligated animals developed mechanical and heat hypersensitivity during total period of study. Soleus muscle weight as well as CDK5 mRNA levels (less than ~ 0.4 fold) was decreased and GSK-3β mRNA levels (up to ~ 7 folds) increased in L5 ligated animals.

Conclusions: These results showed enhanced muscle atrophy processes following peripheral nerve damage and might provide a useful approach to study underlying muscle mechanisms associated with clinical neuropathic pain syndromes.

No MeSH data available.


Related in: MedlinePlus