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Complex Inflammation mRNA-Related Response in ALS Is Region Dependent.

Berjaoui S, Povedano M, Garcia-Esparcia P, Carmona M, Aso E, Ferrer I - Neural Plast. (2015)

Bottom Line: Increased numbers of astrocytes and activated microglia are found in the anterior horn of the spinal cord and pyramidal tracts.Significant increased expression of TLR7, CTSS, and CTSC mRNA and a trend to increased expression of IL10RA, TGFB1, and TGFB2 are found in the anterior lumbar spinal cord in ALS cases compared to control cases, whereas C1QTNF7 and TNFRSF1A mRNA expression levels are significantly decreased.IL-6 immunoreactivity is found in scattered monocyte-derived macrophages/microglia and TNF-α in a few cells of unknown origin in ALS cases.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuropathology, Bellvitge University Hospital, University of Barcelona, CIBERNED (Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas), 08907 L'Hospitalet de Llobregat, Spain.

ABSTRACT
Inflammatory changes are analyzed in the anterior spinal cord and frontal cortex area 8 in typical spinal-predominant ALS cases. Increased numbers of astrocytes and activated microglia are found in the anterior horn of the spinal cord and pyramidal tracts. Significant increased expression of TLR7, CTSS, and CTSC mRNA and a trend to increased expression of IL10RA, TGFB1, and TGFB2 are found in the anterior lumbar spinal cord in ALS cases compared to control cases, whereas C1QTNF7 and TNFRSF1A mRNA expression levels are significantly decreased. IL6 is significantly upregulated and IL1B shows a nonsignificant increased expression in frontal cortex area 8 in ALS cases. IL-6 immunoreactivity is found in scattered monocyte-derived macrophages/microglia and TNF-α in a few cells of unknown origin in ALS cases. Increased expression and abnormal distribution of IL-1β occurred in motor neurons of the lumbar spinal cord in ALS. Strong IL-10 immunoreactivity colocalizes with TDP-43-positive inclusions in motor neurons in ALS cases. The present observations show a complex participation of cytokines and mediators of the inflammatory response in ALS consistent with increased proinflammatory cytokines and sequestration of anti-inflammatory IL-10 in affected neurons.

No MeSH data available.


Related in: MedlinePlus

Double-labelling immunofluorescence and confocal microscopy show partial colocalization of IL-10 (green, a, d, g) and phosphorylated TDP-43 Ser409-410 (red, b, e, h) in globular and skein-like inclusions in motor neurons in ALS cases; merge (c, f, i); nuclei (blue) are with DRAQ5; bar = 25 µm.
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fig3: Double-labelling immunofluorescence and confocal microscopy show partial colocalization of IL-10 (green, a, d, g) and phosphorylated TDP-43 Ser409-410 (red, b, e, h) in globular and skein-like inclusions in motor neurons in ALS cases; merge (c, f, i); nuclei (blue) are with DRAQ5; bar = 25 µm.

Mentions: Double-labelling immunofluorescence and confocal microscopy further supported colocalization of IL-10 and phosphorylated TDP-43 Ser409-410 in globular and skein-like inclusions of motor neurons in ALS, involving about 80% of TDP-43-immunoreactive inclusions (Figure 3). No increased IL-10 immunoreactivity has been seen in glial cells containing phosphorylated TDP-43 Ser409-410 inclusions.


Complex Inflammation mRNA-Related Response in ALS Is Region Dependent.

Berjaoui S, Povedano M, Garcia-Esparcia P, Carmona M, Aso E, Ferrer I - Neural Plast. (2015)

Double-labelling immunofluorescence and confocal microscopy show partial colocalization of IL-10 (green, a, d, g) and phosphorylated TDP-43 Ser409-410 (red, b, e, h) in globular and skein-like inclusions in motor neurons in ALS cases; merge (c, f, i); nuclei (blue) are with DRAQ5; bar = 25 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537753&req=5

fig3: Double-labelling immunofluorescence and confocal microscopy show partial colocalization of IL-10 (green, a, d, g) and phosphorylated TDP-43 Ser409-410 (red, b, e, h) in globular and skein-like inclusions in motor neurons in ALS cases; merge (c, f, i); nuclei (blue) are with DRAQ5; bar = 25 µm.
Mentions: Double-labelling immunofluorescence and confocal microscopy further supported colocalization of IL-10 and phosphorylated TDP-43 Ser409-410 in globular and skein-like inclusions of motor neurons in ALS, involving about 80% of TDP-43-immunoreactive inclusions (Figure 3). No increased IL-10 immunoreactivity has been seen in glial cells containing phosphorylated TDP-43 Ser409-410 inclusions.

Bottom Line: Increased numbers of astrocytes and activated microglia are found in the anterior horn of the spinal cord and pyramidal tracts.Significant increased expression of TLR7, CTSS, and CTSC mRNA and a trend to increased expression of IL10RA, TGFB1, and TGFB2 are found in the anterior lumbar spinal cord in ALS cases compared to control cases, whereas C1QTNF7 and TNFRSF1A mRNA expression levels are significantly decreased.IL-6 immunoreactivity is found in scattered monocyte-derived macrophages/microglia and TNF-α in a few cells of unknown origin in ALS cases.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuropathology, Bellvitge University Hospital, University of Barcelona, CIBERNED (Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas), 08907 L'Hospitalet de Llobregat, Spain.

ABSTRACT
Inflammatory changes are analyzed in the anterior spinal cord and frontal cortex area 8 in typical spinal-predominant ALS cases. Increased numbers of astrocytes and activated microglia are found in the anterior horn of the spinal cord and pyramidal tracts. Significant increased expression of TLR7, CTSS, and CTSC mRNA and a trend to increased expression of IL10RA, TGFB1, and TGFB2 are found in the anterior lumbar spinal cord in ALS cases compared to control cases, whereas C1QTNF7 and TNFRSF1A mRNA expression levels are significantly decreased. IL6 is significantly upregulated and IL1B shows a nonsignificant increased expression in frontal cortex area 8 in ALS cases. IL-6 immunoreactivity is found in scattered monocyte-derived macrophages/microglia and TNF-α in a few cells of unknown origin in ALS cases. Increased expression and abnormal distribution of IL-1β occurred in motor neurons of the lumbar spinal cord in ALS. Strong IL-10 immunoreactivity colocalizes with TDP-43-positive inclusions in motor neurons in ALS cases. The present observations show a complex participation of cytokines and mediators of the inflammatory response in ALS consistent with increased proinflammatory cytokines and sequestration of anti-inflammatory IL-10 in affected neurons.

No MeSH data available.


Related in: MedlinePlus