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Epicatechin Reduces Striatal MPP⁺-Induced Damage in Rats through Slight Increases in SOD-Cu,Zn Activity.

Rubio-Osornio M, Gorostieta-Salas E, Montes S, Pérez-Severiano F, Rubio C, Gómez C, Ríos C, Guevara J - Oxid Med Cell Longev (2015)

Bottom Line: Cu/Zn-dependent superoxide dismutase (Cu/Zn-SOD) activity was significantly (P < 0.05) reduced as a consequence of MPP(+) damage.Such effects persisted in animals injured with MPP(+).The results show that EC is effective against MPP(+)-induced biochemical and behavioral damage, which is possible by an increase in Cu/Zn-SOD activity.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio Experimental de Enfermedades Neurodegenerativas, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, 14269 Mexico City, Mexico.

ABSTRACT
Parkinson's disease is a neurodegenerative disorder characterized by movement alterations caused by reduced dopaminergic neurotransmission in the nigrostriatal pathway, presumably by oxidative stress (OS). MPP(+) intrastriatal injection leads to the overproduction of free radicals (FR). The increasing formation of FR produces OS, a decline in dopamine (DA) content, and behavioral disorders. Epicatechin (EC) has shown the ability to be FR scavenger, an antioxidant enzyme inductor, a redox state modulator, and transition metal chelator. Acute administration of 100 mg/kg of EC significantly prevented (P < 0.05) the circling MPP(+)-induced behavior (10 μg/8 μL). Likewise, EC significantly (P < 0.05) reduced the formation of fluorescent lipid products caused by MPP(+). MPP(+) injection produced (P < 0.05) increased enzymatic activity of the constitutive nitric oxide synthase (cNOS). This effect was blocked with acute EC pretreatment. Cu/Zn-dependent superoxide dismutase (Cu/Zn-SOD) activity was significantly (P < 0.05) reduced as a consequence of MPP(+) damage. EC produced a slight increase (≈20%) in Cu/Zn-SOD activity in the control group. Such effects persisted in animals injured with MPP(+). The results show that EC is effective against MPP(+)-induced biochemical and behavioral damage, which is possible by an increase in Cu/Zn-SOD activity.

No MeSH data available.


Related in: MedlinePlus

EC effect on striatal Ca2+-dependent and Ca2+-independent nitric oxide synthase activity. The Ca2+-dependent NOS activity was significantly increased after MPP+ treatment. The results are expressed as mean ± S.E.M. of 5–7 animals per group in ng L-citrulline/500 mg of protein/30 min. ∗P < 0.05, two-way ANOVA followed by Tukey's test.
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fig3: EC effect on striatal Ca2+-dependent and Ca2+-independent nitric oxide synthase activity. The Ca2+-dependent NOS activity was significantly increased after MPP+ treatment. The results are expressed as mean ± S.E.M. of 5–7 animals per group in ng L-citrulline/500 mg of protein/30 min. ∗P < 0.05, two-way ANOVA followed by Tukey's test.

Mentions: NOS striatal enzymatic activity was also assayed 6 hours after MPP+ infusion. The control group showed levels of Ca2+-dependent NOS activity (constitutive nitric oxide synthase (cNOS)) (3.46 ± 0.68, ng L-citrulline/500 mg protein/30 min), similar to those of the EC pretreated group (4.51 ± 0.69). However, NOS activity was statistically found to increase (P < 0.05) (8.86 ± 1.48) in the MPP+-treated group. Acute oral, 100 mg/kg, administration of EC attenuated the effect of MPP+ on Ca2+-dependent NOS activity (6.30 ± 0.82). Ca2+-independent NOS activity remained unchanged in the striatal tissue from all groups studied in this time and no statistical differences were found among groups (Figure 3).


Epicatechin Reduces Striatal MPP⁺-Induced Damage in Rats through Slight Increases in SOD-Cu,Zn Activity.

Rubio-Osornio M, Gorostieta-Salas E, Montes S, Pérez-Severiano F, Rubio C, Gómez C, Ríos C, Guevara J - Oxid Med Cell Longev (2015)

EC effect on striatal Ca2+-dependent and Ca2+-independent nitric oxide synthase activity. The Ca2+-dependent NOS activity was significantly increased after MPP+ treatment. The results are expressed as mean ± S.E.M. of 5–7 animals per group in ng L-citrulline/500 mg of protein/30 min. ∗P < 0.05, two-way ANOVA followed by Tukey's test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537749&req=5

fig3: EC effect on striatal Ca2+-dependent and Ca2+-independent nitric oxide synthase activity. The Ca2+-dependent NOS activity was significantly increased after MPP+ treatment. The results are expressed as mean ± S.E.M. of 5–7 animals per group in ng L-citrulline/500 mg of protein/30 min. ∗P < 0.05, two-way ANOVA followed by Tukey's test.
Mentions: NOS striatal enzymatic activity was also assayed 6 hours after MPP+ infusion. The control group showed levels of Ca2+-dependent NOS activity (constitutive nitric oxide synthase (cNOS)) (3.46 ± 0.68, ng L-citrulline/500 mg protein/30 min), similar to those of the EC pretreated group (4.51 ± 0.69). However, NOS activity was statistically found to increase (P < 0.05) (8.86 ± 1.48) in the MPP+-treated group. Acute oral, 100 mg/kg, administration of EC attenuated the effect of MPP+ on Ca2+-dependent NOS activity (6.30 ± 0.82). Ca2+-independent NOS activity remained unchanged in the striatal tissue from all groups studied in this time and no statistical differences were found among groups (Figure 3).

Bottom Line: Cu/Zn-dependent superoxide dismutase (Cu/Zn-SOD) activity was significantly (P < 0.05) reduced as a consequence of MPP(+) damage.Such effects persisted in animals injured with MPP(+).The results show that EC is effective against MPP(+)-induced biochemical and behavioral damage, which is possible by an increase in Cu/Zn-SOD activity.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio Experimental de Enfermedades Neurodegenerativas, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, 14269 Mexico City, Mexico.

ABSTRACT
Parkinson's disease is a neurodegenerative disorder characterized by movement alterations caused by reduced dopaminergic neurotransmission in the nigrostriatal pathway, presumably by oxidative stress (OS). MPP(+) intrastriatal injection leads to the overproduction of free radicals (FR). The increasing formation of FR produces OS, a decline in dopamine (DA) content, and behavioral disorders. Epicatechin (EC) has shown the ability to be FR scavenger, an antioxidant enzyme inductor, a redox state modulator, and transition metal chelator. Acute administration of 100 mg/kg of EC significantly prevented (P < 0.05) the circling MPP(+)-induced behavior (10 μg/8 μL). Likewise, EC significantly (P < 0.05) reduced the formation of fluorescent lipid products caused by MPP(+). MPP(+) injection produced (P < 0.05) increased enzymatic activity of the constitutive nitric oxide synthase (cNOS). This effect was blocked with acute EC pretreatment. Cu/Zn-dependent superoxide dismutase (Cu/Zn-SOD) activity was significantly (P < 0.05) reduced as a consequence of MPP(+) damage. EC produced a slight increase (≈20%) in Cu/Zn-SOD activity in the control group. Such effects persisted in animals injured with MPP(+). The results show that EC is effective against MPP(+)-induced biochemical and behavioral damage, which is possible by an increase in Cu/Zn-SOD activity.

No MeSH data available.


Related in: MedlinePlus