Limits...
Binding of CXCL8/IL-8 to Mycobacterium tuberculosis Modulates the Innate Immune Response.

Krupa A, Fol M, Dziadek BR, Kepka E, Wojciechowska D, Brzostek A, Torzewska A, Dziadek J, Baughman RP, Griffith D, Kurdowska AK - Mediators Inflamm. (2015)

Bottom Line: In addition, we have shown that significant amount of IL-8 present in the blood of TB patients associates with erythrocytes.Finally, we have noted that IL-8 is the major chemokine responsible for recruiting T lymphocytes (CD3(+), CD4(+), and CD8(+) T cells).In summary, our data suggest that the association of IL-8 with M. tb molecules may modify and possibly enhance the innate immune response in patients with TB.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Biology, University of Texas Health Science Center at Tyler, Tyler, TX 75708, USA ; Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland.

ABSTRACT
Interleukin-8 (IL-8) has been implicated in the pathogenesis of several human respiratory diseases, including tuberculosis (TB). Importantly and in direct relevance to the objectives of this report quite a few findings suggest that the presence of IL-8 may be beneficial for the host. IL-8 may aid with mounting an adequate response during infection with Mycobacterium tuberculosis (M. tb); however, the underlying mechanism remains largely unknown. The major goal of our study was to investigate the contribution of IL-8 to the inflammatory processes that are typically elicited in patients with TB. We have shown for the first time that IL-8 can directly bind to tubercle bacilli. We have also demonstrated that association of IL-8 with M. tb molecules leads to the augmentation of the ability of leukocytes (neutrophils and macrophages) to phagocyte and kill these bacilli. In addition, we have shown that significant amount of IL-8 present in the blood of TB patients associates with erythrocytes. Finally, we have noted that IL-8 is the major chemokine responsible for recruiting T lymphocytes (CD3(+), CD4(+), and CD8(+) T cells). In summary, our data suggest that the association of IL-8 with M. tb molecules may modify and possibly enhance the innate immune response in patients with TB.

No MeSH data available.


Related in: MedlinePlus

IL-8-induced chemotaxis of T lymphocytes.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4537748&req=5

fig6: IL-8-induced chemotaxis of T lymphocytes.

Mentions: Since the role of IL-8 as a chemoattractant for T lymphocytes remains controversial [38–41], we evaluated the ability of IL-8 to recruit these cells. We observed that IL-8 induced a significant chemotaxis of T lymphocytes (Figure 6). This response was highly reproducible and dose dependent (Figure 6). Moreover, both CD4+ and CD8+ T cell subsets play a central role in developing resistance to M. tb [42]; thus, CD4+ and CD8+ T lymphocytes were also tested in the chemotactic assay (Figure 6). These cells were isolated using magnetic beads conjugated to CD4 or CD8 (purity of >95%). We found that both CD4+ and CD3+ T cells reached maximum response at an IL-8 concentration of 10−10 M (Figure 6). The response of CD8+ T lymphocytes was weaker than of other cell types and reached a maximum at an IL-8 concentration of 10−11 M (Figure 6).


Binding of CXCL8/IL-8 to Mycobacterium tuberculosis Modulates the Innate Immune Response.

Krupa A, Fol M, Dziadek BR, Kepka E, Wojciechowska D, Brzostek A, Torzewska A, Dziadek J, Baughman RP, Griffith D, Kurdowska AK - Mediators Inflamm. (2015)

IL-8-induced chemotaxis of T lymphocytes.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537748&req=5

fig6: IL-8-induced chemotaxis of T lymphocytes.
Mentions: Since the role of IL-8 as a chemoattractant for T lymphocytes remains controversial [38–41], we evaluated the ability of IL-8 to recruit these cells. We observed that IL-8 induced a significant chemotaxis of T lymphocytes (Figure 6). This response was highly reproducible and dose dependent (Figure 6). Moreover, both CD4+ and CD8+ T cell subsets play a central role in developing resistance to M. tb [42]; thus, CD4+ and CD8+ T lymphocytes were also tested in the chemotactic assay (Figure 6). These cells were isolated using magnetic beads conjugated to CD4 or CD8 (purity of >95%). We found that both CD4+ and CD3+ T cells reached maximum response at an IL-8 concentration of 10−10 M (Figure 6). The response of CD8+ T lymphocytes was weaker than of other cell types and reached a maximum at an IL-8 concentration of 10−11 M (Figure 6).

Bottom Line: In addition, we have shown that significant amount of IL-8 present in the blood of TB patients associates with erythrocytes.Finally, we have noted that IL-8 is the major chemokine responsible for recruiting T lymphocytes (CD3(+), CD4(+), and CD8(+) T cells).In summary, our data suggest that the association of IL-8 with M. tb molecules may modify and possibly enhance the innate immune response in patients with TB.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Biology, University of Texas Health Science Center at Tyler, Tyler, TX 75708, USA ; Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland.

ABSTRACT
Interleukin-8 (IL-8) has been implicated in the pathogenesis of several human respiratory diseases, including tuberculosis (TB). Importantly and in direct relevance to the objectives of this report quite a few findings suggest that the presence of IL-8 may be beneficial for the host. IL-8 may aid with mounting an adequate response during infection with Mycobacterium tuberculosis (M. tb); however, the underlying mechanism remains largely unknown. The major goal of our study was to investigate the contribution of IL-8 to the inflammatory processes that are typically elicited in patients with TB. We have shown for the first time that IL-8 can directly bind to tubercle bacilli. We have also demonstrated that association of IL-8 with M. tb molecules leads to the augmentation of the ability of leukocytes (neutrophils and macrophages) to phagocyte and kill these bacilli. In addition, we have shown that significant amount of IL-8 present in the blood of TB patients associates with erythrocytes. Finally, we have noted that IL-8 is the major chemokine responsible for recruiting T lymphocytes (CD3(+), CD4(+), and CD8(+) T cells). In summary, our data suggest that the association of IL-8 with M. tb molecules may modify and possibly enhance the innate immune response in patients with TB.

No MeSH data available.


Related in: MedlinePlus