Limits...
Anti-Inflammatory Property of Plantago major Leaf Extract Reduces the Inflammatory Reaction in Experimental Acetaminophen-Induced Liver Injury.

Hussan F, Mansor AS, Hassan SN, Tengku Nor Effendy Kamaruddin TN, Budin SB, Othman F - Evid Based Complement Alternat Med (2015)

Bottom Line: The levels of liver transaminases were measured at day 1 and day 7 after APAP induction.The ALT and AST levels were significantly higher in the APAP groups at day 1 whereas the enzyme levels of all groups showed no significant difference at day 7.The extracts treatment significantly reduced the proinflammatory cytokine levels and significantly increased the 11β-HSD type 1 enzyme activity (p < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia.

ABSTRACT
Hepatic injury induces inflammatory process and cell necrosis. Plantago major is traditionally used for various diseases. This study aimed to determine the anti-inflammatory property of P. major leaf extracts on inflammatory reaction following acetaminophen (APAP) hepatotoxicity. Thirty male Sprague-Dawley rats were divided into 5 groups, namely, normal control (C), APAP, aqueous (APAP + AQ), methanol (APAP + MT), and ethanol (APAP + ET) extract treated groups. All APAP groups received oral APAP (2 g/kg) at day 0. Then, 1000 mg/kg dose of P. major extracts was given for six days. The levels of liver transaminases were measured at day 1 and day 7 after APAP induction. At day 7, the blood and liver tissue were collected to determine plasma cytokines and tissue 11β-HSD type 1 enzyme. The in vitro anti-inflammatory activities of methanol, ethanol, and aqueous extracts were 26.74 ± 1.6%, 21.69 ± 2.81%, and 12.23 ± 3.15%, respectively. The ALT and AST levels were significantly higher in the APAP groups at day 1 whereas the enzyme levels of all groups showed no significant difference at day 7. The extracts treatment significantly reduced the proinflammatory cytokine levels and significantly increased the 11β-HSD type 1 enzyme activity (p < 0.05). In conclusion, the P. major extracts attenuate the inflammatory reaction following APAP-induced liver injury.

No MeSH data available.


Related in: MedlinePlus

Effects of three different P. major extracts on the activity of 11β-HSD type 1 enzyme in APAP-induced liver tissue;  ∗significant difference from the APAP group (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4537734&req=5

fig3: Effects of three different P. major extracts on the activity of 11β-HSD type 1 enzyme in APAP-induced liver tissue;  ∗significant difference from the APAP group (p < 0.05).

Mentions: The activity of 11β-HSD type 1 enzyme in all groups was shown in Figure 3. The enzyme activity between the control and APAP groups showed no significant difference whereas the activity was significantly reduced in APAP group compared to the APAP + MT and APAP + ET groups (p < 0.05). Therefore, the methanol and ethanol extract were able to enhance the enzyme activity following APAP-induced liver injury.


Anti-Inflammatory Property of Plantago major Leaf Extract Reduces the Inflammatory Reaction in Experimental Acetaminophen-Induced Liver Injury.

Hussan F, Mansor AS, Hassan SN, Tengku Nor Effendy Kamaruddin TN, Budin SB, Othman F - Evid Based Complement Alternat Med (2015)

Effects of three different P. major extracts on the activity of 11β-HSD type 1 enzyme in APAP-induced liver tissue;  ∗significant difference from the APAP group (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537734&req=5

fig3: Effects of three different P. major extracts on the activity of 11β-HSD type 1 enzyme in APAP-induced liver tissue;  ∗significant difference from the APAP group (p < 0.05).
Mentions: The activity of 11β-HSD type 1 enzyme in all groups was shown in Figure 3. The enzyme activity between the control and APAP groups showed no significant difference whereas the activity was significantly reduced in APAP group compared to the APAP + MT and APAP + ET groups (p < 0.05). Therefore, the methanol and ethanol extract were able to enhance the enzyme activity following APAP-induced liver injury.

Bottom Line: The levels of liver transaminases were measured at day 1 and day 7 after APAP induction.The ALT and AST levels were significantly higher in the APAP groups at day 1 whereas the enzyme levels of all groups showed no significant difference at day 7.The extracts treatment significantly reduced the proinflammatory cytokine levels and significantly increased the 11β-HSD type 1 enzyme activity (p < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia.

ABSTRACT
Hepatic injury induces inflammatory process and cell necrosis. Plantago major is traditionally used for various diseases. This study aimed to determine the anti-inflammatory property of P. major leaf extracts on inflammatory reaction following acetaminophen (APAP) hepatotoxicity. Thirty male Sprague-Dawley rats were divided into 5 groups, namely, normal control (C), APAP, aqueous (APAP + AQ), methanol (APAP + MT), and ethanol (APAP + ET) extract treated groups. All APAP groups received oral APAP (2 g/kg) at day 0. Then, 1000 mg/kg dose of P. major extracts was given for six days. The levels of liver transaminases were measured at day 1 and day 7 after APAP induction. At day 7, the blood and liver tissue were collected to determine plasma cytokines and tissue 11β-HSD type 1 enzyme. The in vitro anti-inflammatory activities of methanol, ethanol, and aqueous extracts were 26.74 ± 1.6%, 21.69 ± 2.81%, and 12.23 ± 3.15%, respectively. The ALT and AST levels were significantly higher in the APAP groups at day 1 whereas the enzyme levels of all groups showed no significant difference at day 7. The extracts treatment significantly reduced the proinflammatory cytokine levels and significantly increased the 11β-HSD type 1 enzyme activity (p < 0.05). In conclusion, the P. major extracts attenuate the inflammatory reaction following APAP-induced liver injury.

No MeSH data available.


Related in: MedlinePlus