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Assessment of Vascular Endothelial Growth Factor in Fresh versus Frozen Platelet Rich Plasma.

Hosny N, Goubran F, BadrEldin Hasan B, Kamel N - J Blood Transfus (2015)

Bottom Line: Platelets count was significantly increased compared to baseline whole blood values in all fresh and frozen PRP samples (p value was <0.05).No significant difference was found between VEGF concentrations after activating fresh and frozen-thawed PRP samples for one and three weeks by calcium alone or calcium with thrombin, and also no significant difference was found when freezing period was extended from one to three weeks.Our results showed that platelets count does not correlate with variable levels of VEGF.

View Article: PubMed Central - PubMed

Affiliation: Clinical Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt.

ABSTRACT
Platelet rich plasma (PRP) is hemoconcentration with platelets concentration above baseline values and high concentration of many growth factors. The aim of this study was to assess freezing effect on vascular endothelial growth factor (VEGF) release from PRP using two different activation methods to simplify its use in different clinical applications. PRP was prepared using two-centrifugation steps method from 12 qualified blood donors. VEGF concentrations were measured in fresh PRP and after freezing/thawing for one and three weeks with two methods of activation using (i) calcium gluconate and (ii) calcium gluconate and thrombin. Platelets count was significantly increased compared to baseline whole blood values in all fresh and frozen PRP samples (p value was <0.05). No significant difference was found between VEGF concentrations after activating fresh and frozen-thawed PRP samples for one and three weeks by calcium alone or calcium with thrombin, and also no significant difference was found when freezing period was extended from one to three weeks. Our results showed that platelets count does not correlate with variable levels of VEGF. PRP could be prepared once and preserved frozen for at least three weeks for the next treatment sessions and activation with thrombin addition to calcium will not augment the growth factor release.

No MeSH data available.


Correlations between VEGF concentrations and platelets count in different PRP activation and storage periods. Rs, Spearman rank correlation coefficient.
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Related In: Results  -  Collection


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fig1: Correlations between VEGF concentrations and platelets count in different PRP activation and storage periods. Rs, Spearman rank correlation coefficient.

Mentions: Using the Spearman rank correlation coefficient, variation in VEGF levels did not significantly correlate with variations in PRP platelets count (they showed only fair degree of linear relationship where Rs ranged from 0.34 to 0.50) (Figure 1).


Assessment of Vascular Endothelial Growth Factor in Fresh versus Frozen Platelet Rich Plasma.

Hosny N, Goubran F, BadrEldin Hasan B, Kamel N - J Blood Transfus (2015)

Correlations between VEGF concentrations and platelets count in different PRP activation and storage periods. Rs, Spearman rank correlation coefficient.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537731&req=5

fig1: Correlations between VEGF concentrations and platelets count in different PRP activation and storage periods. Rs, Spearman rank correlation coefficient.
Mentions: Using the Spearman rank correlation coefficient, variation in VEGF levels did not significantly correlate with variations in PRP platelets count (they showed only fair degree of linear relationship where Rs ranged from 0.34 to 0.50) (Figure 1).

Bottom Line: Platelets count was significantly increased compared to baseline whole blood values in all fresh and frozen PRP samples (p value was <0.05).No significant difference was found between VEGF concentrations after activating fresh and frozen-thawed PRP samples for one and three weeks by calcium alone or calcium with thrombin, and also no significant difference was found when freezing period was extended from one to three weeks.Our results showed that platelets count does not correlate with variable levels of VEGF.

View Article: PubMed Central - PubMed

Affiliation: Clinical Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt.

ABSTRACT
Platelet rich plasma (PRP) is hemoconcentration with platelets concentration above baseline values and high concentration of many growth factors. The aim of this study was to assess freezing effect on vascular endothelial growth factor (VEGF) release from PRP using two different activation methods to simplify its use in different clinical applications. PRP was prepared using two-centrifugation steps method from 12 qualified blood donors. VEGF concentrations were measured in fresh PRP and after freezing/thawing for one and three weeks with two methods of activation using (i) calcium gluconate and (ii) calcium gluconate and thrombin. Platelets count was significantly increased compared to baseline whole blood values in all fresh and frozen PRP samples (p value was <0.05). No significant difference was found between VEGF concentrations after activating fresh and frozen-thawed PRP samples for one and three weeks by calcium alone or calcium with thrombin, and also no significant difference was found when freezing period was extended from one to three weeks. Our results showed that platelets count does not correlate with variable levels of VEGF. PRP could be prepared once and preserved frozen for at least three weeks for the next treatment sessions and activation with thrombin addition to calcium will not augment the growth factor release.

No MeSH data available.