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Oral Administration of Ganoderma lucidum to Lead-Exposed Rats Protects Erythrocytes against Hemolysis: Implicates to Anti-Anemia.

Hossain S, Bhowmick S, Islam S, Rozario L, Jahan S, Hassan M, Sarkar M, Choudhury BK, Ahmed S, Shahjalal H - Evid Based Complement Alternat Med (2015)

Bottom Line: The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively.The levels of TNFα and LPO also were determined from hepatic tissues.Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.

ABSTRACT
We studied the effect of chronic oral exposure to lead acetate (PbA) on the sensitivity of RBC to hemolysis and whether the sensitivity could be decreased by feeding the rats with extract of medicinal mushroom Ganoderma lucidum. Three groups of rats, control, PbA-exposed, and G. lucidum (Gl)+PbA, were used. PbA (3 mM) was administered via drinking water and G. lucidum extract by gavage at 300 mg/Kg BW/day for 12 weeks. Afterwards, the rats were killed and washed RBCs were subjected to hemolysis in the presence of Fenton's reagents. Hemolysis was determined by estimating the amount of released hemoglobin. The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively. The levels of TNFα and LPO also were determined from hepatic tissues. The RBCs of PbA-exposed rats displayed significantly higher sensitivity to hemolysis than those of the Gl+PbA rats. The levels of LPO increased and GSH decreased in the RBCs, with concomitant increases in the levels of hepatic TNFα and LPO in the PbA-exposed rats. The degree of hemolysis was significantly low in the RBCs of Gl+PbA rats, concurrently with amelioration of hepatic parameters. Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

No MeSH data available.


Related in: MedlinePlus

Effect of α-tocopherol on oxidative stress (FR: Fenton's reagents) induced hemolysis. Results are mean ± SE (n = 6 ~ 8). Bars with different alphabets are significantly different at P < 0.05. α-tocopherol (α-TF) dose-dependently inhibited the degree of hemolysis, as indicated by the gradual decreases in the levels of released hemoglobin. 1% SDS was used as positive control. Data were analyzed with one-way ANOVA, with Fisher's PLSD for post hoc comparison. (−) indicates absence. (+) indicates presence.
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fig3: Effect of α-tocopherol on oxidative stress (FR: Fenton's reagents) induced hemolysis. Results are mean ± SE (n = 6 ~ 8). Bars with different alphabets are significantly different at P < 0.05. α-tocopherol (α-TF) dose-dependently inhibited the degree of hemolysis, as indicated by the gradual decreases in the levels of released hemoglobin. 1% SDS was used as positive control. Data were analyzed with one-way ANOVA, with Fisher's PLSD for post hoc comparison. (−) indicates absence. (+) indicates presence.

Mentions: To understand whether the antioxidative defense was involved in lowering the extent of hemolysis, the RBCs from the non-Pb exposed rats were subjected to the oxidative stress by Fenton's reagents in the absence (0 μM) or presence of α-tocopherol (10–100 μM). 1% SDS was used as positive control of hemolysis (Figure 3). Hemolysis data were normalized to those of the positive control. The levels of hemolysis were decreased with increases in the concentrations α-tocopherol in the samples, as indicated by the decreased amount of released hemoglobin in the buffer (Figure 3).


Oral Administration of Ganoderma lucidum to Lead-Exposed Rats Protects Erythrocytes against Hemolysis: Implicates to Anti-Anemia.

Hossain S, Bhowmick S, Islam S, Rozario L, Jahan S, Hassan M, Sarkar M, Choudhury BK, Ahmed S, Shahjalal H - Evid Based Complement Alternat Med (2015)

Effect of α-tocopherol on oxidative stress (FR: Fenton's reagents) induced hemolysis. Results are mean ± SE (n = 6 ~ 8). Bars with different alphabets are significantly different at P < 0.05. α-tocopherol (α-TF) dose-dependently inhibited the degree of hemolysis, as indicated by the gradual decreases in the levels of released hemoglobin. 1% SDS was used as positive control. Data were analyzed with one-way ANOVA, with Fisher's PLSD for post hoc comparison. (−) indicates absence. (+) indicates presence.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537730&req=5

fig3: Effect of α-tocopherol on oxidative stress (FR: Fenton's reagents) induced hemolysis. Results are mean ± SE (n = 6 ~ 8). Bars with different alphabets are significantly different at P < 0.05. α-tocopherol (α-TF) dose-dependently inhibited the degree of hemolysis, as indicated by the gradual decreases in the levels of released hemoglobin. 1% SDS was used as positive control. Data were analyzed with one-way ANOVA, with Fisher's PLSD for post hoc comparison. (−) indicates absence. (+) indicates presence.
Mentions: To understand whether the antioxidative defense was involved in lowering the extent of hemolysis, the RBCs from the non-Pb exposed rats were subjected to the oxidative stress by Fenton's reagents in the absence (0 μM) or presence of α-tocopherol (10–100 μM). 1% SDS was used as positive control of hemolysis (Figure 3). Hemolysis data were normalized to those of the positive control. The levels of hemolysis were decreased with increases in the concentrations α-tocopherol in the samples, as indicated by the decreased amount of released hemoglobin in the buffer (Figure 3).

Bottom Line: The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively.The levels of TNFα and LPO also were determined from hepatic tissues.Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.

ABSTRACT
We studied the effect of chronic oral exposure to lead acetate (PbA) on the sensitivity of RBC to hemolysis and whether the sensitivity could be decreased by feeding the rats with extract of medicinal mushroom Ganoderma lucidum. Three groups of rats, control, PbA-exposed, and G. lucidum (Gl)+PbA, were used. PbA (3 mM) was administered via drinking water and G. lucidum extract by gavage at 300 mg/Kg BW/day for 12 weeks. Afterwards, the rats were killed and washed RBCs were subjected to hemolysis in the presence of Fenton's reagents. Hemolysis was determined by estimating the amount of released hemoglobin. The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively. The levels of TNFα and LPO also were determined from hepatic tissues. The RBCs of PbA-exposed rats displayed significantly higher sensitivity to hemolysis than those of the Gl+PbA rats. The levels of LPO increased and GSH decreased in the RBCs, with concomitant increases in the levels of hepatic TNFα and LPO in the PbA-exposed rats. The degree of hemolysis was significantly low in the RBCs of Gl+PbA rats, concurrently with amelioration of hepatic parameters. Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

No MeSH data available.


Related in: MedlinePlus