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Oral Administration of Ganoderma lucidum to Lead-Exposed Rats Protects Erythrocytes against Hemolysis: Implicates to Anti-Anemia.

Hossain S, Bhowmick S, Islam S, Rozario L, Jahan S, Hassan M, Sarkar M, Choudhury BK, Ahmed S, Shahjalal H - Evid Based Complement Alternat Med (2015)

Bottom Line: The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively.The levels of TNFα and LPO also were determined from hepatic tissues.Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.

ABSTRACT
We studied the effect of chronic oral exposure to lead acetate (PbA) on the sensitivity of RBC to hemolysis and whether the sensitivity could be decreased by feeding the rats with extract of medicinal mushroom Ganoderma lucidum. Three groups of rats, control, PbA-exposed, and G. lucidum (Gl)+PbA, were used. PbA (3 mM) was administered via drinking water and G. lucidum extract by gavage at 300 mg/Kg BW/day for 12 weeks. Afterwards, the rats were killed and washed RBCs were subjected to hemolysis in the presence of Fenton's reagents. Hemolysis was determined by estimating the amount of released hemoglobin. The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively. The levels of TNFα and LPO also were determined from hepatic tissues. The RBCs of PbA-exposed rats displayed significantly higher sensitivity to hemolysis than those of the Gl+PbA rats. The levels of LPO increased and GSH decreased in the RBCs, with concomitant increases in the levels of hepatic TNFα and LPO in the PbA-exposed rats. The degree of hemolysis was significantly low in the RBCs of Gl+PbA rats, concurrently with amelioration of hepatic parameters. Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

No MeSH data available.


Related in: MedlinePlus

Effect of Pb on hemolysis. Results are mean ± SE (n = 6 ~ 8), each with duplicate determinations. Bars with different alphabets are significantly different at P < 0.05. Data were analyzed with one-way ANOVA, with Fisher's PLSD for post hoc comparison.
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fig2: Effect of Pb on hemolysis. Results are mean ± SE (n = 6 ~ 8), each with duplicate determinations. Bars with different alphabets are significantly different at P < 0.05. Data were analyzed with one-way ANOVA, with Fisher's PLSD for post hoc comparison.

Mentions: The susceptibility of RBCs to the oxidative stress-induced (Fenton's reagents) hemolysis was measured in all erythrocyte samples of the controls, PbA-exposed and G. lucidum+PbA-fed rats (Figure 2). The RBCs of the PbA-exposed rats displayed the highest sensitivity to Fenton's reagents-instigated hemolysis. It was ~51% higher (P < 0.05), as compared to that of the control. However, the oral administration of G. lucidum extract to the PbA-fed rats significantly reduced the degree of hemolysis in the rats (G. lucidum+PbA) (Figure 2).


Oral Administration of Ganoderma lucidum to Lead-Exposed Rats Protects Erythrocytes against Hemolysis: Implicates to Anti-Anemia.

Hossain S, Bhowmick S, Islam S, Rozario L, Jahan S, Hassan M, Sarkar M, Choudhury BK, Ahmed S, Shahjalal H - Evid Based Complement Alternat Med (2015)

Effect of Pb on hemolysis. Results are mean ± SE (n = 6 ~ 8), each with duplicate determinations. Bars with different alphabets are significantly different at P < 0.05. Data were analyzed with one-way ANOVA, with Fisher's PLSD for post hoc comparison.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537730&req=5

fig2: Effect of Pb on hemolysis. Results are mean ± SE (n = 6 ~ 8), each with duplicate determinations. Bars with different alphabets are significantly different at P < 0.05. Data were analyzed with one-way ANOVA, with Fisher's PLSD for post hoc comparison.
Mentions: The susceptibility of RBCs to the oxidative stress-induced (Fenton's reagents) hemolysis was measured in all erythrocyte samples of the controls, PbA-exposed and G. lucidum+PbA-fed rats (Figure 2). The RBCs of the PbA-exposed rats displayed the highest sensitivity to Fenton's reagents-instigated hemolysis. It was ~51% higher (P < 0.05), as compared to that of the control. However, the oral administration of G. lucidum extract to the PbA-fed rats significantly reduced the degree of hemolysis in the rats (G. lucidum+PbA) (Figure 2).

Bottom Line: The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively.The levels of TNFα and LPO also were determined from hepatic tissues.Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.

ABSTRACT
We studied the effect of chronic oral exposure to lead acetate (PbA) on the sensitivity of RBC to hemolysis and whether the sensitivity could be decreased by feeding the rats with extract of medicinal mushroom Ganoderma lucidum. Three groups of rats, control, PbA-exposed, and G. lucidum (Gl)+PbA, were used. PbA (3 mM) was administered via drinking water and G. lucidum extract by gavage at 300 mg/Kg BW/day for 12 weeks. Afterwards, the rats were killed and washed RBCs were subjected to hemolysis in the presence of Fenton's reagents. Hemolysis was determined by estimating the amount of released hemoglobin. The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively. The levels of TNFα and LPO also were determined from hepatic tissues. The RBCs of PbA-exposed rats displayed significantly higher sensitivity to hemolysis than those of the Gl+PbA rats. The levels of LPO increased and GSH decreased in the RBCs, with concomitant increases in the levels of hepatic TNFα and LPO in the PbA-exposed rats. The degree of hemolysis was significantly low in the RBCs of Gl+PbA rats, concurrently with amelioration of hepatic parameters. Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

No MeSH data available.


Related in: MedlinePlus