Limits...
Oral Administration of Ganoderma lucidum to Lead-Exposed Rats Protects Erythrocytes against Hemolysis: Implicates to Anti-Anemia.

Hossain S, Bhowmick S, Islam S, Rozario L, Jahan S, Hassan M, Sarkar M, Choudhury BK, Ahmed S, Shahjalal H - Evid Based Complement Alternat Med (2015)

Bottom Line: The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively.The levels of TNFα and LPO also were determined from hepatic tissues.Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.

ABSTRACT
We studied the effect of chronic oral exposure to lead acetate (PbA) on the sensitivity of RBC to hemolysis and whether the sensitivity could be decreased by feeding the rats with extract of medicinal mushroom Ganoderma lucidum. Three groups of rats, control, PbA-exposed, and G. lucidum (Gl)+PbA, were used. PbA (3 mM) was administered via drinking water and G. lucidum extract by gavage at 300 mg/Kg BW/day for 12 weeks. Afterwards, the rats were killed and washed RBCs were subjected to hemolysis in the presence of Fenton's reagents. Hemolysis was determined by estimating the amount of released hemoglobin. The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively. The levels of TNFα and LPO also were determined from hepatic tissues. The RBCs of PbA-exposed rats displayed significantly higher sensitivity to hemolysis than those of the Gl+PbA rats. The levels of LPO increased and GSH decreased in the RBCs, with concomitant increases in the levels of hepatic TNFα and LPO in the PbA-exposed rats. The degree of hemolysis was significantly low in the RBCs of Gl+PbA rats, concurrently with amelioration of hepatic parameters. Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

No MeSH data available.


Related in: MedlinePlus

Experimental design. RBCs: red blood cells; GSH: reduced glutathione; Hb: hemoglobin; TNFα: tumor necrosis factor α.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4537730&req=5

fig1: Experimental design. RBCs: red blood cells; GSH: reduced glutathione; Hb: hemoglobin; TNFα: tumor necrosis factor α.

Mentions: Wistar rats obtained from animal breeding colony of the icddr,b, Dhaka, were used in the present study. The animals were fed on standard pellet diet and maintained under controlled laboratory conditions (12 h light: 12 h dark; temperature 25 ± 2°C; relative humidity 50 ± 10%). The inbred second generation rats (15 weeks old, 180–200 g body weight, [BW]) were randomly divided into three groups: the control group (n = 6), lead acetate- (PbA-) exposed group (n = 8), and G. lucidum-fed PbA-exposed group (Gl+PbA, n = 7) (Figure 1). The PbA-exposed group was orally fed PbA at 3 mM prepared drinking water. The extract of G. lucidum dissolved in distilled water was orally administered at 300 mg/Kg BW/day. The control group was orally fed a similar volume of the dH2O alone. Oral administration of PbA and/or G. lucidum was continued for 12 weeks. The rats were cared for and killed in accordance with the guidelines of laboratory animals and approved by the Institutional Animal Ethical Committee at Jahangirnagar University, Savar, Dhaka, Bangladesh.


Oral Administration of Ganoderma lucidum to Lead-Exposed Rats Protects Erythrocytes against Hemolysis: Implicates to Anti-Anemia.

Hossain S, Bhowmick S, Islam S, Rozario L, Jahan S, Hassan M, Sarkar M, Choudhury BK, Ahmed S, Shahjalal H - Evid Based Complement Alternat Med (2015)

Experimental design. RBCs: red blood cells; GSH: reduced glutathione; Hb: hemoglobin; TNFα: tumor necrosis factor α.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537730&req=5

fig1: Experimental design. RBCs: red blood cells; GSH: reduced glutathione; Hb: hemoglobin; TNFα: tumor necrosis factor α.
Mentions: Wistar rats obtained from animal breeding colony of the icddr,b, Dhaka, were used in the present study. The animals were fed on standard pellet diet and maintained under controlled laboratory conditions (12 h light: 12 h dark; temperature 25 ± 2°C; relative humidity 50 ± 10%). The inbred second generation rats (15 weeks old, 180–200 g body weight, [BW]) were randomly divided into three groups: the control group (n = 6), lead acetate- (PbA-) exposed group (n = 8), and G. lucidum-fed PbA-exposed group (Gl+PbA, n = 7) (Figure 1). The PbA-exposed group was orally fed PbA at 3 mM prepared drinking water. The extract of G. lucidum dissolved in distilled water was orally administered at 300 mg/Kg BW/day. The control group was orally fed a similar volume of the dH2O alone. Oral administration of PbA and/or G. lucidum was continued for 12 weeks. The rats were cared for and killed in accordance with the guidelines of laboratory animals and approved by the Institutional Animal Ethical Committee at Jahangirnagar University, Savar, Dhaka, Bangladesh.

Bottom Line: The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively.The levels of TNFα and LPO also were determined from hepatic tissues.Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Laboratory of Alternative Medicine and Behavioral Neurosciences, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.

ABSTRACT
We studied the effect of chronic oral exposure to lead acetate (PbA) on the sensitivity of RBC to hemolysis and whether the sensitivity could be decreased by feeding the rats with extract of medicinal mushroom Ganoderma lucidum. Three groups of rats, control, PbA-exposed, and G. lucidum (Gl)+PbA, were used. PbA (3 mM) was administered via drinking water and G. lucidum extract by gavage at 300 mg/Kg BW/day for 12 weeks. Afterwards, the rats were killed and washed RBCs were subjected to hemolysis in the presence of Fenton's reagents. Hemolysis was determined by estimating the amount of released hemoglobin. The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively. The levels of TNFα and LPO also were determined from hepatic tissues. The RBCs of PbA-exposed rats displayed significantly higher sensitivity to hemolysis than those of the Gl+PbA rats. The levels of LPO increased and GSH decreased in the RBCs, with concomitant increases in the levels of hepatic TNFα and LPO in the PbA-exposed rats. The degree of hemolysis was significantly low in the RBCs of Gl+PbA rats, concurrently with amelioration of hepatic parameters. Finally, the study suggests that PbA-induced-hemolysis and related oxidative-toxicity might be minimized by consumption of G. lucidum.

No MeSH data available.


Related in: MedlinePlus