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Krill Oil Ameliorates Mitochondrial Dysfunctions in Rats Treated with High-Fat Diet.

Ferramosca A, Conte A, Zara V - Biomed Res Int (2015)

Bottom Line: On the other hand, krill oil, a novel dietary supplement of n-3 PUFAs, has the ability to improve lipid and glucose metabolism, exerting possible protective effects against hepatic steatosis.This effect is obtained by stimulating mitochondrial metabolic pathways such as fatty acid oxidation, Krebs cycle, and respiratory chain complexes activity.Modulation of the expression of carrier proteins involved in mitochondrial uncoupling was also observed.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Provinciale Lecce-Monteroni, 73100 Lecce, Italy.

ABSTRACT
In recent years, several studies focused their attention on the role of dietary fats in the pathogenesis of hepatic steatosis. It has been demonstrated that a high-fat diet is able to induce hyperglycemia, hyperinsulinemia, obesity, and nonalcoholic fatty liver disease. On the other hand, krill oil, a novel dietary supplement of n-3 PUFAs, has the ability to improve lipid and glucose metabolism, exerting possible protective effects against hepatic steatosis. In this study we have investigated the effects of krill oil on mitochondrial energetic metabolism in animals fed a high-fat diet. To this end, male Sprague-Dawley rats were divided into three groups and fed for 4 weeks with a standard diet (control group), a diet with 35% fat (HF group), or a high-fat diet supplemented with 2.5% krill oil (HF+KO group). The obtained results suggest that krill oil promotes the burning of fat excess introduced by the high-fat diet. This effect is obtained by stimulating mitochondrial metabolic pathways such as fatty acid oxidation, Krebs cycle, and respiratory chain complexes activity. Modulation of the expression of carrier proteins involved in mitochondrial uncoupling was also observed. Overall, krill oil counteracts the negative effects of a high-fat diet on mitochondrial energetic metabolism.

No MeSH data available.


Related in: MedlinePlus

Liver samples were stored in neutral formaldehyde and embedded in paraffin wax. Sections (15 μm) were stained with hematoxylin and eosin.
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Related In: Results  -  Collection


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fig1: Liver samples were stored in neutral formaldehyde and embedded in paraffin wax. Sections (15 μm) were stained with hematoxylin and eosin.

Mentions: No significant difference was detected in the serum lipid (triglyceride and cholesterol) levels between the three groups of animals. On the contrary, liver lipid content was dramatically enhanced in HF animals, thus reflecting the presence of hepatic steatosis (Figure 1). The supplementation with KO of the HF diet reversed this effect, thereby keeping the lipid concentrations similar to those found in control rats. Accordingly, the liver histologic examination revealed a beginning of microvesicular fat depositions only in HF rats.


Krill Oil Ameliorates Mitochondrial Dysfunctions in Rats Treated with High-Fat Diet.

Ferramosca A, Conte A, Zara V - Biomed Res Int (2015)

Liver samples were stored in neutral formaldehyde and embedded in paraffin wax. Sections (15 μm) were stained with hematoxylin and eosin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537729&req=5

fig1: Liver samples were stored in neutral formaldehyde and embedded in paraffin wax. Sections (15 μm) were stained with hematoxylin and eosin.
Mentions: No significant difference was detected in the serum lipid (triglyceride and cholesterol) levels between the three groups of animals. On the contrary, liver lipid content was dramatically enhanced in HF animals, thus reflecting the presence of hepatic steatosis (Figure 1). The supplementation with KO of the HF diet reversed this effect, thereby keeping the lipid concentrations similar to those found in control rats. Accordingly, the liver histologic examination revealed a beginning of microvesicular fat depositions only in HF rats.

Bottom Line: On the other hand, krill oil, a novel dietary supplement of n-3 PUFAs, has the ability to improve lipid and glucose metabolism, exerting possible protective effects against hepatic steatosis.This effect is obtained by stimulating mitochondrial metabolic pathways such as fatty acid oxidation, Krebs cycle, and respiratory chain complexes activity.Modulation of the expression of carrier proteins involved in mitochondrial uncoupling was also observed.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Provinciale Lecce-Monteroni, 73100 Lecce, Italy.

ABSTRACT
In recent years, several studies focused their attention on the role of dietary fats in the pathogenesis of hepatic steatosis. It has been demonstrated that a high-fat diet is able to induce hyperglycemia, hyperinsulinemia, obesity, and nonalcoholic fatty liver disease. On the other hand, krill oil, a novel dietary supplement of n-3 PUFAs, has the ability to improve lipid and glucose metabolism, exerting possible protective effects against hepatic steatosis. In this study we have investigated the effects of krill oil on mitochondrial energetic metabolism in animals fed a high-fat diet. To this end, male Sprague-Dawley rats were divided into three groups and fed for 4 weeks with a standard diet (control group), a diet with 35% fat (HF group), or a high-fat diet supplemented with 2.5% krill oil (HF+KO group). The obtained results suggest that krill oil promotes the burning of fat excess introduced by the high-fat diet. This effect is obtained by stimulating mitochondrial metabolic pathways such as fatty acid oxidation, Krebs cycle, and respiratory chain complexes activity. Modulation of the expression of carrier proteins involved in mitochondrial uncoupling was also observed. Overall, krill oil counteracts the negative effects of a high-fat diet on mitochondrial energetic metabolism.

No MeSH data available.


Related in: MedlinePlus