Limits...
Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media.

Weyand B, Israelowitz M, Kramer J, Bodmer C, Noehre M, Strauss S, Schmälzlin E, Gille C, von Schroeder HP, Reimers K, Vogt PM - Biomed Res Int (2015)

Bottom Line: The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities.Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2).Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.

ABSTRACT
A three-dimensional computational fluid dynamics- (CFD-) model based on a differential pressure laminar flow bioreactor prototype was developed to further examine performance under changing culture conditions. Cell growth inside scaffolds was simulated by decreasing intrinsic permeability values and led to pressure build-up in the upper culture chamber. Pressure release by an integrated bypass system allowed continuation of culture. The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities. Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2). Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

No MeSH data available.


Related in: MedlinePlus

(a and b) Scanning electron microscopy shows the dense fiber network of the collagen matrix with mesenchymal stem cells after 10 days of dynamic culture in the bioreactor. (c) Cross-section of collagen matrix stained with DAPI fluorescence stain shows collagen fibrous network (in green) with adipose mesenchymal stem cells (blue: nuclei of MSC) cultured in the bioreactor after 14 days of culture. (d) Fluorescence microscopy shows vital mesenchymal cells longitudinally aligned after 1-week dynamic culture in perfusion bioreactor (cell vitality stain).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4537716&req=5

fig6: (a and b) Scanning electron microscopy shows the dense fiber network of the collagen matrix with mesenchymal stem cells after 10 days of dynamic culture in the bioreactor. (c) Cross-section of collagen matrix stained with DAPI fluorescence stain shows collagen fibrous network (in green) with adipose mesenchymal stem cells (blue: nuclei of MSC) cultured in the bioreactor after 14 days of culture. (d) Fluorescence microscopy shows vital mesenchymal cells longitudinally aligned after 1-week dynamic culture in perfusion bioreactor (cell vitality stain).

Mentions: Results of the CFD simulation are presented in Figures 3–6. Figure 3 shows the pressure distribution in the bioreactor system simulated with various scaffold permeability. With the bypass system of the bioreactor closed, the pressure difference between the upper and lower parts of the culture chamber separated by the scaffold increases with decreasing intrinsic scaffold permeability as seen in Figures 3(a)–3(c). The “pressure drop” accounts for 6.5 Pa for a permeability of 5 ∗ 10−11 and 63 Pa for a permeability of 5 ∗ 10−12 and increases up to 360 Pa when permeability decreases to 5 ∗ 10−13. Opening the bypass system results in pressure release, and pressure values in the upper and lower part of the culture chamber adjust to a pressure difference of about 3.3 Pa, as seen here in Figure 3(d) for the simulation with the lowest scaffold permeability 5 ∗ 10−13.


Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media.

Weyand B, Israelowitz M, Kramer J, Bodmer C, Noehre M, Strauss S, Schmälzlin E, Gille C, von Schroeder HP, Reimers K, Vogt PM - Biomed Res Int (2015)

(a and b) Scanning electron microscopy shows the dense fiber network of the collagen matrix with mesenchymal stem cells after 10 days of dynamic culture in the bioreactor. (c) Cross-section of collagen matrix stained with DAPI fluorescence stain shows collagen fibrous network (in green) with adipose mesenchymal stem cells (blue: nuclei of MSC) cultured in the bioreactor after 14 days of culture. (d) Fluorescence microscopy shows vital mesenchymal cells longitudinally aligned after 1-week dynamic culture in perfusion bioreactor (cell vitality stain).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537716&req=5

fig6: (a and b) Scanning electron microscopy shows the dense fiber network of the collagen matrix with mesenchymal stem cells after 10 days of dynamic culture in the bioreactor. (c) Cross-section of collagen matrix stained with DAPI fluorescence stain shows collagen fibrous network (in green) with adipose mesenchymal stem cells (blue: nuclei of MSC) cultured in the bioreactor after 14 days of culture. (d) Fluorescence microscopy shows vital mesenchymal cells longitudinally aligned after 1-week dynamic culture in perfusion bioreactor (cell vitality stain).
Mentions: Results of the CFD simulation are presented in Figures 3–6. Figure 3 shows the pressure distribution in the bioreactor system simulated with various scaffold permeability. With the bypass system of the bioreactor closed, the pressure difference between the upper and lower parts of the culture chamber separated by the scaffold increases with decreasing intrinsic scaffold permeability as seen in Figures 3(a)–3(c). The “pressure drop” accounts for 6.5 Pa for a permeability of 5 ∗ 10−11 and 63 Pa for a permeability of 5 ∗ 10−12 and increases up to 360 Pa when permeability decreases to 5 ∗ 10−13. Opening the bypass system results in pressure release, and pressure values in the upper and lower part of the culture chamber adjust to a pressure difference of about 3.3 Pa, as seen here in Figure 3(d) for the simulation with the lowest scaffold permeability 5 ∗ 10−13.

Bottom Line: The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities.Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2).Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.

ABSTRACT
A three-dimensional computational fluid dynamics- (CFD-) model based on a differential pressure laminar flow bioreactor prototype was developed to further examine performance under changing culture conditions. Cell growth inside scaffolds was simulated by decreasing intrinsic permeability values and led to pressure build-up in the upper culture chamber. Pressure release by an integrated bypass system allowed continuation of culture. The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities. Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2). Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

No MeSH data available.


Related in: MedlinePlus