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Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media.

Weyand B, Israelowitz M, Kramer J, Bodmer C, Noehre M, Strauss S, Schmälzlin E, Gille C, von Schroeder HP, Reimers K, Vogt PM - Biomed Res Int (2015)

Bottom Line: The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities.Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2).Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.

ABSTRACT
A three-dimensional computational fluid dynamics- (CFD-) model based on a differential pressure laminar flow bioreactor prototype was developed to further examine performance under changing culture conditions. Cell growth inside scaffolds was simulated by decreasing intrinsic permeability values and led to pressure build-up in the upper culture chamber. Pressure release by an integrated bypass system allowed continuation of culture. The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities. Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2). Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

No MeSH data available.


Related in: MedlinePlus

Mesh of the bioreactor created with the Star-CCM+ program.
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fig2: Mesh of the bioreactor created with the Star-CCM+ program.

Mentions: The laminar flow bioreactor and the oxygen sensor system are being presented in Figure 1. Figure 1(a) shows a schematic view of the whole setup, Figure 1(b) shows the bioreactor during an experimental course, and Figure 1(c) demonstrates the scaffold holder device suitable for simultaneous cultivation of up to seven cylindrical scaffolds with a diameter of 10 mm. The lateral positioned bypass system, which can be seen in Figure 1(a) number 4, was designed to release pressure build-up and prevent high shear forces inside the scaffolds during cell growth inside the porous matrices. For CFD simulation, a mesh of the bioreactor was created with the surface wrapper and polyhedral volume mesher of the Star-CCM+ program which is presented in Figure 2; here the open bypass systems are marked by an arrow. For the closed bypass model, bypasses were blocked by an interface layer which was integrated in the mesh.


Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media.

Weyand B, Israelowitz M, Kramer J, Bodmer C, Noehre M, Strauss S, Schmälzlin E, Gille C, von Schroeder HP, Reimers K, Vogt PM - Biomed Res Int (2015)

Mesh of the bioreactor created with the Star-CCM+ program.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537716&req=5

fig2: Mesh of the bioreactor created with the Star-CCM+ program.
Mentions: The laminar flow bioreactor and the oxygen sensor system are being presented in Figure 1. Figure 1(a) shows a schematic view of the whole setup, Figure 1(b) shows the bioreactor during an experimental course, and Figure 1(c) demonstrates the scaffold holder device suitable for simultaneous cultivation of up to seven cylindrical scaffolds with a diameter of 10 mm. The lateral positioned bypass system, which can be seen in Figure 1(a) number 4, was designed to release pressure build-up and prevent high shear forces inside the scaffolds during cell growth inside the porous matrices. For CFD simulation, a mesh of the bioreactor was created with the surface wrapper and polyhedral volume mesher of the Star-CCM+ program which is presented in Figure 2; here the open bypass systems are marked by an arrow. For the closed bypass model, bypasses were blocked by an interface layer which was integrated in the mesh.

Bottom Line: The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities.Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2).Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.

ABSTRACT
A three-dimensional computational fluid dynamics- (CFD-) model based on a differential pressure laminar flow bioreactor prototype was developed to further examine performance under changing culture conditions. Cell growth inside scaffolds was simulated by decreasing intrinsic permeability values and led to pressure build-up in the upper culture chamber. Pressure release by an integrated bypass system allowed continuation of culture. The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities. Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2). Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

No MeSH data available.


Related in: MedlinePlus