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Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media.

Weyand B, Israelowitz M, Kramer J, Bodmer C, Noehre M, Strauss S, Schmälzlin E, Gille C, von Schroeder HP, Reimers K, Vogt PM - Biomed Res Int (2015)

Bottom Line: The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities.Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2).Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.

ABSTRACT
A three-dimensional computational fluid dynamics- (CFD-) model based on a differential pressure laminar flow bioreactor prototype was developed to further examine performance under changing culture conditions. Cell growth inside scaffolds was simulated by decreasing intrinsic permeability values and led to pressure build-up in the upper culture chamber. Pressure release by an integrated bypass system allowed continuation of culture. The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities. Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2). Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

No MeSH data available.


Related in: MedlinePlus

(a) Schematics of differential pressure laminar flow bioreactor system with integrated laser-based oxygen sensor system (from PCT/EP2011/067344/WO 2012/045756). 1: Control box, 2: pressure ports, 3: bioreactor vessel with scaffold holder, 4: integrated bypass system, 5: sampling probe (for medium analysis), 6: culture medium reservoir, 7: peristaltic pump, 8: laser light with filters and lenses, 9: photomultiplier (light detector), 10: temperature sensor, 11: OPAL system with built-in sinusoidal frequency generator and lock-in amplifier, 12: computer with software, and →: medium flow direction. (b) Photo of system. (c) Scaffold holder suitable for cultivation of up to seven cylindrical scaffolds.
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fig1: (a) Schematics of differential pressure laminar flow bioreactor system with integrated laser-based oxygen sensor system (from PCT/EP2011/067344/WO 2012/045756). 1: Control box, 2: pressure ports, 3: bioreactor vessel with scaffold holder, 4: integrated bypass system, 5: sampling probe (for medium analysis), 6: culture medium reservoir, 7: peristaltic pump, 8: laser light with filters and lenses, 9: photomultiplier (light detector), 10: temperature sensor, 11: OPAL system with built-in sinusoidal frequency generator and lock-in amplifier, 12: computer with software, and →: medium flow direction. (b) Photo of system. (c) Scaffold holder suitable for cultivation of up to seven cylindrical scaffolds.

Mentions: The bioreactor was equipped with a laser-based oxygen measurement system called “OPAL,” which measures oxygen-dependent phosphorescence lifetime of microbeads (50 μm diameter), and uses a two-frequency modulation technique to eliminate interference by background fluorescence [37]. The system, which actually is intended to be used with fluorescence microscopes, has been described in detail previously [37–39]. In order to detect the signal of the spherical oxygen microsensors, the photomultiplier unit was linked to a camera objective and placed in front of a window, which had been integrated in the bioreactor wall (see Figure 1).


Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media.

Weyand B, Israelowitz M, Kramer J, Bodmer C, Noehre M, Strauss S, Schmälzlin E, Gille C, von Schroeder HP, Reimers K, Vogt PM - Biomed Res Int (2015)

(a) Schematics of differential pressure laminar flow bioreactor system with integrated laser-based oxygen sensor system (from PCT/EP2011/067344/WO 2012/045756). 1: Control box, 2: pressure ports, 3: bioreactor vessel with scaffold holder, 4: integrated bypass system, 5: sampling probe (for medium analysis), 6: culture medium reservoir, 7: peristaltic pump, 8: laser light with filters and lenses, 9: photomultiplier (light detector), 10: temperature sensor, 11: OPAL system with built-in sinusoidal frequency generator and lock-in amplifier, 12: computer with software, and →: medium flow direction. (b) Photo of system. (c) Scaffold holder suitable for cultivation of up to seven cylindrical scaffolds.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4537716&req=5

fig1: (a) Schematics of differential pressure laminar flow bioreactor system with integrated laser-based oxygen sensor system (from PCT/EP2011/067344/WO 2012/045756). 1: Control box, 2: pressure ports, 3: bioreactor vessel with scaffold holder, 4: integrated bypass system, 5: sampling probe (for medium analysis), 6: culture medium reservoir, 7: peristaltic pump, 8: laser light with filters and lenses, 9: photomultiplier (light detector), 10: temperature sensor, 11: OPAL system with built-in sinusoidal frequency generator and lock-in amplifier, 12: computer with software, and →: medium flow direction. (b) Photo of system. (c) Scaffold holder suitable for cultivation of up to seven cylindrical scaffolds.
Mentions: The bioreactor was equipped with a laser-based oxygen measurement system called “OPAL,” which measures oxygen-dependent phosphorescence lifetime of microbeads (50 μm diameter), and uses a two-frequency modulation technique to eliminate interference by background fluorescence [37]. The system, which actually is intended to be used with fluorescence microscopes, has been described in detail previously [37–39]. In order to detect the signal of the spherical oxygen microsensors, the photomultiplier unit was linked to a camera objective and placed in front of a window, which had been integrated in the bioreactor wall (see Figure 1).

Bottom Line: The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities.Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2).Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.

ABSTRACT
A three-dimensional computational fluid dynamics- (CFD-) model based on a differential pressure laminar flow bioreactor prototype was developed to further examine performance under changing culture conditions. Cell growth inside scaffolds was simulated by decreasing intrinsic permeability values and led to pressure build-up in the upper culture chamber. Pressure release by an integrated bypass system allowed continuation of culture. The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities. Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2). Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

No MeSH data available.


Related in: MedlinePlus