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Microbiological Analysis of Necrosols Collected from Urban Cemeteries in Poland.

Całkosiński I, Płoneczka-Janeczko K, Ostapska M, Dudek K, Gamian A, Rypuła K - Biomed Res Int (2015)

Bottom Line: The fungi Penicillium spp. and Aspergillus spp. were isolated from 51% and 6.4% of samples, respectively.Other bacterial species were in the ground cemetery relatively sparse.Sampling depth was not correlated with bacterial growth (p > 0.05), but it was correlated with several differences in microbiota composition (superficial versus deep layer).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neurotoxicology and Environmental Diagnosis, Faculty of Health Science, Wroclaw Medical University, 51-618 Wroclaw, Poland.

ABSTRACT
Decomposition of organic matter is the primary function in the soil ecosystem, which involves bacteria and fungi. Soil microbial content depends on many factors, and secondary biological and chemical contaminations change and affect environmental feedback. Little work has been done to estimate the microbiological risk for cemetery employees and visitors. The potential risk of infection for people in the cemetery is primarily associated with injury and wound contamination during performing the work. The aim of this study was to analyze the microbiota of cemetery soil obtained from cemeteries and bacterial composition in selected soil layers encountered by gravediggers and cemetery caretakers. The most common bacterial pathogens were Enterococcus spp. (80.6%), Bacillus spp. (77.4%), and E. coli (45.1%). The fungi Penicillium spp. and Aspergillus spp. were isolated from 51% and 6.4% of samples, respectively. Other bacterial species were in the ground cemetery relatively sparse. Sampling depth was not correlated with bacterial growth (p > 0.05), but it was correlated with several differences in microbiota composition (superficial versus deep layer).

No MeSH data available.


Related in: MedlinePlus

(a) The presence of bacterial microflora in the soil of burial at a depth of 0.15–0.20 m collected from the five necropolises (A–E). (b) The presence of bacterial microflora in the soil of burial at a depth of 2.0 m collected from the five necropolises (A–E). BS: Bacillus spp., EnS: Enterococcus spp., EsS: Escherichia spp., KES: Klebsiella spp., Enterobacter spp., and Serratia spp., StS: Staphylococcus spp.
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fig3: (a) The presence of bacterial microflora in the soil of burial at a depth of 0.15–0.20 m collected from the five necropolises (A–E). (b) The presence of bacterial microflora in the soil of burial at a depth of 2.0 m collected from the five necropolises (A–E). BS: Bacillus spp., EnS: Enterococcus spp., EsS: Escherichia spp., KES: Klebsiella spp., Enterobacter spp., and Serratia spp., StS: Staphylococcus spp.

Mentions: Figure 3 shows the bacterial growth levels of the two soil layers according to microorganisms identified. Bacillus spp. showed abundant growth (>104 CFU/mL) in samples from all cemeteries, independent of sampling depth. Growth of Enterococcus spp. ranged from <103 to >104 CFU/mL in both soil layers. The growth of Escherichia spp., predominantly E. coli, was greater in samples from the deep soil layer (from 103 to >104 CFU/mL) than in those from the superficial layer (<103 to 103). The KES group was identified in samples from only one grave (at cemetery E), with >104 CFU/mL observed in samples from both soil depths.


Microbiological Analysis of Necrosols Collected from Urban Cemeteries in Poland.

Całkosiński I, Płoneczka-Janeczko K, Ostapska M, Dudek K, Gamian A, Rypuła K - Biomed Res Int (2015)

(a) The presence of bacterial microflora in the soil of burial at a depth of 0.15–0.20 m collected from the five necropolises (A–E). (b) The presence of bacterial microflora in the soil of burial at a depth of 2.0 m collected from the five necropolises (A–E). BS: Bacillus spp., EnS: Enterococcus spp., EsS: Escherichia spp., KES: Klebsiella spp., Enterobacter spp., and Serratia spp., StS: Staphylococcus spp.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537714&req=5

fig3: (a) The presence of bacterial microflora in the soil of burial at a depth of 0.15–0.20 m collected from the five necropolises (A–E). (b) The presence of bacterial microflora in the soil of burial at a depth of 2.0 m collected from the five necropolises (A–E). BS: Bacillus spp., EnS: Enterococcus spp., EsS: Escherichia spp., KES: Klebsiella spp., Enterobacter spp., and Serratia spp., StS: Staphylococcus spp.
Mentions: Figure 3 shows the bacterial growth levels of the two soil layers according to microorganisms identified. Bacillus spp. showed abundant growth (>104 CFU/mL) in samples from all cemeteries, independent of sampling depth. Growth of Enterococcus spp. ranged from <103 to >104 CFU/mL in both soil layers. The growth of Escherichia spp., predominantly E. coli, was greater in samples from the deep soil layer (from 103 to >104 CFU/mL) than in those from the superficial layer (<103 to 103). The KES group was identified in samples from only one grave (at cemetery E), with >104 CFU/mL observed in samples from both soil depths.

Bottom Line: The fungi Penicillium spp. and Aspergillus spp. were isolated from 51% and 6.4% of samples, respectively.Other bacterial species were in the ground cemetery relatively sparse.Sampling depth was not correlated with bacterial growth (p > 0.05), but it was correlated with several differences in microbiota composition (superficial versus deep layer).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neurotoxicology and Environmental Diagnosis, Faculty of Health Science, Wroclaw Medical University, 51-618 Wroclaw, Poland.

ABSTRACT
Decomposition of organic matter is the primary function in the soil ecosystem, which involves bacteria and fungi. Soil microbial content depends on many factors, and secondary biological and chemical contaminations change and affect environmental feedback. Little work has been done to estimate the microbiological risk for cemetery employees and visitors. The potential risk of infection for people in the cemetery is primarily associated with injury and wound contamination during performing the work. The aim of this study was to analyze the microbiota of cemetery soil obtained from cemeteries and bacterial composition in selected soil layers encountered by gravediggers and cemetery caretakers. The most common bacterial pathogens were Enterococcus spp. (80.6%), Bacillus spp. (77.4%), and E. coli (45.1%). The fungi Penicillium spp. and Aspergillus spp. were isolated from 51% and 6.4% of samples, respectively. Other bacterial species were in the ground cemetery relatively sparse. Sampling depth was not correlated with bacterial growth (p > 0.05), but it was correlated with several differences in microbiota composition (superficial versus deep layer).

No MeSH data available.


Related in: MedlinePlus