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Microbiological Analysis of Necrosols Collected from Urban Cemeteries in Poland.

Całkosiński I, Płoneczka-Janeczko K, Ostapska M, Dudek K, Gamian A, Rypuła K - Biomed Res Int (2015)

Bottom Line: The fungi Penicillium spp. and Aspergillus spp. were isolated from 51% and 6.4% of samples, respectively.Other bacterial species were in the ground cemetery relatively sparse.Sampling depth was not correlated with bacterial growth (p > 0.05), but it was correlated with several differences in microbiota composition (superficial versus deep layer).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neurotoxicology and Environmental Diagnosis, Faculty of Health Science, Wroclaw Medical University, 51-618 Wroclaw, Poland.

ABSTRACT
Decomposition of organic matter is the primary function in the soil ecosystem, which involves bacteria and fungi. Soil microbial content depends on many factors, and secondary biological and chemical contaminations change and affect environmental feedback. Little work has been done to estimate the microbiological risk for cemetery employees and visitors. The potential risk of infection for people in the cemetery is primarily associated with injury and wound contamination during performing the work. The aim of this study was to analyze the microbiota of cemetery soil obtained from cemeteries and bacterial composition in selected soil layers encountered by gravediggers and cemetery caretakers. The most common bacterial pathogens were Enterococcus spp. (80.6%), Bacillus spp. (77.4%), and E. coli (45.1%). The fungi Penicillium spp. and Aspergillus spp. were isolated from 51% and 6.4% of samples, respectively. Other bacterial species were in the ground cemetery relatively sparse. Sampling depth was not correlated with bacterial growth (p > 0.05), but it was correlated with several differences in microbiota composition (superficial versus deep layer).

No MeSH data available.


Related in: MedlinePlus

The diagram of sampling for analysis from one place of burial: on each of two depths (I, II), in each case the soil collected at the points P1–P4 (the corners of the grave) and P5 (a point defined at the intersection of the diagonals).
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fig2: The diagram of sampling for analysis from one place of burial: on each of two depths (I, II), in each case the soil collected at the points P1–P4 (the corners of the grave) and P5 (a point defined at the intersection of the diagonals).

Mentions: Samples for microbiological examination were collected from a total of 155 burial sites (A, n = 45; B, n = 20; C, n = 30; D, n = 35; E, n = 25) between summer 2013 and spring 2014. Each site was sampled only once. Soil samples were collected during simple grave preparation (reused places after 20 years) using 150 mL containers (Medlab, Poland). At two depths (0.15–0.20 m and 2 m), 200 g soil was taken from each of five points (four grave corners P1–P4 and center P5, identified by the intersection of diagonal lines from the corners; Figure 2). Samples were packed for shipping and transported by automobile to the EPI-Vet Diagnostic Laboratory, Faculty of Veterinary Medicine, Wroclaw, for microbial analysis. The laboratory implements a quality management system (ISO/IEC 17025:2005 + API:2007 + AC:2007).


Microbiological Analysis of Necrosols Collected from Urban Cemeteries in Poland.

Całkosiński I, Płoneczka-Janeczko K, Ostapska M, Dudek K, Gamian A, Rypuła K - Biomed Res Int (2015)

The diagram of sampling for analysis from one place of burial: on each of two depths (I, II), in each case the soil collected at the points P1–P4 (the corners of the grave) and P5 (a point defined at the intersection of the diagonals).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537714&req=5

fig2: The diagram of sampling for analysis from one place of burial: on each of two depths (I, II), in each case the soil collected at the points P1–P4 (the corners of the grave) and P5 (a point defined at the intersection of the diagonals).
Mentions: Samples for microbiological examination were collected from a total of 155 burial sites (A, n = 45; B, n = 20; C, n = 30; D, n = 35; E, n = 25) between summer 2013 and spring 2014. Each site was sampled only once. Soil samples were collected during simple grave preparation (reused places after 20 years) using 150 mL containers (Medlab, Poland). At two depths (0.15–0.20 m and 2 m), 200 g soil was taken from each of five points (four grave corners P1–P4 and center P5, identified by the intersection of diagonal lines from the corners; Figure 2). Samples were packed for shipping and transported by automobile to the EPI-Vet Diagnostic Laboratory, Faculty of Veterinary Medicine, Wroclaw, for microbial analysis. The laboratory implements a quality management system (ISO/IEC 17025:2005 + API:2007 + AC:2007).

Bottom Line: The fungi Penicillium spp. and Aspergillus spp. were isolated from 51% and 6.4% of samples, respectively.Other bacterial species were in the ground cemetery relatively sparse.Sampling depth was not correlated with bacterial growth (p > 0.05), but it was correlated with several differences in microbiota composition (superficial versus deep layer).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neurotoxicology and Environmental Diagnosis, Faculty of Health Science, Wroclaw Medical University, 51-618 Wroclaw, Poland.

ABSTRACT
Decomposition of organic matter is the primary function in the soil ecosystem, which involves bacteria and fungi. Soil microbial content depends on many factors, and secondary biological and chemical contaminations change and affect environmental feedback. Little work has been done to estimate the microbiological risk for cemetery employees and visitors. The potential risk of infection for people in the cemetery is primarily associated with injury and wound contamination during performing the work. The aim of this study was to analyze the microbiota of cemetery soil obtained from cemeteries and bacterial composition in selected soil layers encountered by gravediggers and cemetery caretakers. The most common bacterial pathogens were Enterococcus spp. (80.6%), Bacillus spp. (77.4%), and E. coli (45.1%). The fungi Penicillium spp. and Aspergillus spp. were isolated from 51% and 6.4% of samples, respectively. Other bacterial species were in the ground cemetery relatively sparse. Sampling depth was not correlated with bacterial growth (p > 0.05), but it was correlated with several differences in microbiota composition (superficial versus deep layer).

No MeSH data available.


Related in: MedlinePlus