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ω-3 PUFAs and Resveratrol Differently Modulate Acute and Chronic Inflammatory Processes.

Schwager J, Richard N, Riegger C, Salem N - Biomed Res Int (2015)

Bottom Line: ω-3 PUFAs and polyphenols have multiple effects on inflammation in vivo and in vitro.Combination of RV and ω-3 PUFAs exerted synergistic effects on CCL5/RANTES and had additive effects on IL-6 or CXCL8/IL-8.Both ω-3 PUFAs and RV reduced catabolic gene expression (e.g., MMPs, ADAMTS-4, IL-1β, and IL-6) in activated chondrocytes.

View Article: PubMed Central - PubMed

Affiliation: DSM Nutritional Products Ltd., Department of Human Nutrition & Health, P.O. Box 2676, 4002 Basel, Switzerland.

ABSTRACT
ω-3 PUFAs and polyphenols have multiple effects on inflammation in vivo and in vitro. The effects of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and resveratrol (RV) were investigated in LPS-stimulated peripheral blood leukocytes (PBLs) (i.e., acute inflammation) and IL-1β activated human chondrocytes (i.e., chronic inflammation). Inflammatory mediators including chemokines, cytokines, interleukins, and PGE2 were measured by multiplex analysis and gene expression was quantified by RT-PCR. In PBLs, RV decreased the secretion of PGE2, CCL5/RANTES, and CXCL8/IL-8 but increased IL-1β, IL-6, and IL-10. In contrast to RV, ω-3 PUFAs augmented the production of PGE2 and CXCL8/IL-8. EPA and DHA similarly affected the pattern of inflammatory mediators. Combination of RV and ω-3 PUFAs exerted synergistic effects on CCL5/RANTES and had additive effects on IL-6 or CXCL8/IL-8. Both ω-3 PUFAs and RV reduced catabolic gene expression (e.g., MMPs, ADAMTS-4, IL-1β, and IL-6) in activated chondrocytes. The data suggest that ω-3 PUFAs and RV differ in the regulation of acute inflammation of peripheral blood leukocytes but have common properties in modulating features related to chronic inflammation of chondrocytes.

No MeSH data available.


Related in: MedlinePlus

Expression of inflammatory genes in activated PBLs. Cells were activated with LPS with or without the indicated substances for 12 h. Gene expression was quantified by RT-PCR and the data expressed as fold change compared to levels observed in unstimulated cells. Mean ± standard errors of duplicates from three individuals are given. ∗p < 0.05, #p < 0.01, and §p < 0.001 (LPS only versus LPS + substance).
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fig2: Expression of inflammatory genes in activated PBLs. Cells were activated with LPS with or without the indicated substances for 12 h. Gene expression was quantified by RT-PCR and the data expressed as fold change compared to levels observed in unstimulated cells. Mean ± standard errors of duplicates from three individuals are given. ∗p < 0.05, #p < 0.01, and §p < 0.001 (LPS only versus LPS + substance).

Mentions: By using quantitative RT-PCR, we investigated the impact of the substances on the transcription of inflammatory genes in PBLs after 12 h of LPS-stimulation, when many LPS-responsive genes were still upregulated [19, 27]. RV had only a minor influence on IL-1β mRNA levels, whereas it significantly augmented IL-6 transcription (Figure 2), consistent with the increased IL-6 secretion (Figure 1). Similarly, IL-10 mRNA levels of RV-treated cells matched the higher secretion of IL-10. We observed a significant decrease of CXCL8/IL-8 expression by RV (at 25 μmol/L) (Figure 2). Conversely, CCL5/RANTES gene expression was not induced by LPS-stimulation nor changed by concomitant RV treatment. DHA altered gene expression patterns in a similar way, as it changed the secretion of the respective proteins: IL-6 expression and, to a lesser extent, IL-1β and IL-10 expression were augmented by DHA (at 20 μmol/L) (Figure 2). CXCL8/IL-8 expression was enhanced, when DHA was included at 20 μmol/L in the assay. Collectively, the data indicate that RV and ω-3 PUFAs regulate cytokine and chemokine production at the level of transcription.


ω-3 PUFAs and Resveratrol Differently Modulate Acute and Chronic Inflammatory Processes.

Schwager J, Richard N, Riegger C, Salem N - Biomed Res Int (2015)

Expression of inflammatory genes in activated PBLs. Cells were activated with LPS with or without the indicated substances for 12 h. Gene expression was quantified by RT-PCR and the data expressed as fold change compared to levels observed in unstimulated cells. Mean ± standard errors of duplicates from three individuals are given. ∗p < 0.05, #p < 0.01, and §p < 0.001 (LPS only versus LPS + substance).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537711&req=5

fig2: Expression of inflammatory genes in activated PBLs. Cells were activated with LPS with or without the indicated substances for 12 h. Gene expression was quantified by RT-PCR and the data expressed as fold change compared to levels observed in unstimulated cells. Mean ± standard errors of duplicates from three individuals are given. ∗p < 0.05, #p < 0.01, and §p < 0.001 (LPS only versus LPS + substance).
Mentions: By using quantitative RT-PCR, we investigated the impact of the substances on the transcription of inflammatory genes in PBLs after 12 h of LPS-stimulation, when many LPS-responsive genes were still upregulated [19, 27]. RV had only a minor influence on IL-1β mRNA levels, whereas it significantly augmented IL-6 transcription (Figure 2), consistent with the increased IL-6 secretion (Figure 1). Similarly, IL-10 mRNA levels of RV-treated cells matched the higher secretion of IL-10. We observed a significant decrease of CXCL8/IL-8 expression by RV (at 25 μmol/L) (Figure 2). Conversely, CCL5/RANTES gene expression was not induced by LPS-stimulation nor changed by concomitant RV treatment. DHA altered gene expression patterns in a similar way, as it changed the secretion of the respective proteins: IL-6 expression and, to a lesser extent, IL-1β and IL-10 expression were augmented by DHA (at 20 μmol/L) (Figure 2). CXCL8/IL-8 expression was enhanced, when DHA was included at 20 μmol/L in the assay. Collectively, the data indicate that RV and ω-3 PUFAs regulate cytokine and chemokine production at the level of transcription.

Bottom Line: ω-3 PUFAs and polyphenols have multiple effects on inflammation in vivo and in vitro.Combination of RV and ω-3 PUFAs exerted synergistic effects on CCL5/RANTES and had additive effects on IL-6 or CXCL8/IL-8.Both ω-3 PUFAs and RV reduced catabolic gene expression (e.g., MMPs, ADAMTS-4, IL-1β, and IL-6) in activated chondrocytes.

View Article: PubMed Central - PubMed

Affiliation: DSM Nutritional Products Ltd., Department of Human Nutrition & Health, P.O. Box 2676, 4002 Basel, Switzerland.

ABSTRACT
ω-3 PUFAs and polyphenols have multiple effects on inflammation in vivo and in vitro. The effects of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and resveratrol (RV) were investigated in LPS-stimulated peripheral blood leukocytes (PBLs) (i.e., acute inflammation) and IL-1β activated human chondrocytes (i.e., chronic inflammation). Inflammatory mediators including chemokines, cytokines, interleukins, and PGE2 were measured by multiplex analysis and gene expression was quantified by RT-PCR. In PBLs, RV decreased the secretion of PGE2, CCL5/RANTES, and CXCL8/IL-8 but increased IL-1β, IL-6, and IL-10. In contrast to RV, ω-3 PUFAs augmented the production of PGE2 and CXCL8/IL-8. EPA and DHA similarly affected the pattern of inflammatory mediators. Combination of RV and ω-3 PUFAs exerted synergistic effects on CCL5/RANTES and had additive effects on IL-6 or CXCL8/IL-8. Both ω-3 PUFAs and RV reduced catabolic gene expression (e.g., MMPs, ADAMTS-4, IL-1β, and IL-6) in activated chondrocytes. The data suggest that ω-3 PUFAs and RV differ in the regulation of acute inflammation of peripheral blood leukocytes but have common properties in modulating features related to chronic inflammation of chondrocytes.

No MeSH data available.


Related in: MedlinePlus