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ω-3 PUFAs and Resveratrol Differently Modulate Acute and Chronic Inflammatory Processes.

Schwager J, Richard N, Riegger C, Salem N - Biomed Res Int (2015)

Bottom Line: ω-3 PUFAs and polyphenols have multiple effects on inflammation in vivo and in vitro.Combination of RV and ω-3 PUFAs exerted synergistic effects on CCL5/RANTES and had additive effects on IL-6 or CXCL8/IL-8.Both ω-3 PUFAs and RV reduced catabolic gene expression (e.g., MMPs, ADAMTS-4, IL-1β, and IL-6) in activated chondrocytes.

View Article: PubMed Central - PubMed

Affiliation: DSM Nutritional Products Ltd., Department of Human Nutrition & Health, P.O. Box 2676, 4002 Basel, Switzerland.

ABSTRACT
ω-3 PUFAs and polyphenols have multiple effects on inflammation in vivo and in vitro. The effects of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and resveratrol (RV) were investigated in LPS-stimulated peripheral blood leukocytes (PBLs) (i.e., acute inflammation) and IL-1β activated human chondrocytes (i.e., chronic inflammation). Inflammatory mediators including chemokines, cytokines, interleukins, and PGE2 were measured by multiplex analysis and gene expression was quantified by RT-PCR. In PBLs, RV decreased the secretion of PGE2, CCL5/RANTES, and CXCL8/IL-8 but increased IL-1β, IL-6, and IL-10. In contrast to RV, ω-3 PUFAs augmented the production of PGE2 and CXCL8/IL-8. EPA and DHA similarly affected the pattern of inflammatory mediators. Combination of RV and ω-3 PUFAs exerted synergistic effects on CCL5/RANTES and had additive effects on IL-6 or CXCL8/IL-8. Both ω-3 PUFAs and RV reduced catabolic gene expression (e.g., MMPs, ADAMTS-4, IL-1β, and IL-6) in activated chondrocytes. The data suggest that ω-3 PUFAs and RV differ in the regulation of acute inflammation of peripheral blood leukocytes but have common properties in modulating features related to chronic inflammation of chondrocytes.

No MeSH data available.


Related in: MedlinePlus

Production of inflammatory mediators by activated PBLs and its modulation by RV, DHA, and EPA. PBLs from healthy individuals were activated with LPS in the presence of the indicated substances (in μmol/L) and the inflammatory mediators in supernatants of 24 h cultures were determined in duplicate. The results are expressed as the ratio of substance + LPS-treated cells/LPS-treated cells. Data are given as mean ± SD of experiments obtained from PBL from at least four individuals. The dotted line (at ratio 1) indicates the no-effect level. ¤, ¥ indicate statistically significant differences observed in 50% and >75% of the donors, respectively.
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fig1: Production of inflammatory mediators by activated PBLs and its modulation by RV, DHA, and EPA. PBLs from healthy individuals were activated with LPS in the presence of the indicated substances (in μmol/L) and the inflammatory mediators in supernatants of 24 h cultures were determined in duplicate. The results are expressed as the ratio of substance + LPS-treated cells/LPS-treated cells. Data are given as mean ± SD of experiments obtained from PBL from at least four individuals. The dotted line (at ratio 1) indicates the no-effect level. ¤, ¥ indicate statistically significant differences observed in 50% and >75% of the donors, respectively.

Mentions: In order to investigate the effects of nutrients on acute inflammatory responses, PBLs from different individuals were activated with LPS in the presence of graded amounts of substances. LPS triggered substantial secretion of cytokines, chemokines, and PGE2; some of these differed considerably between individuals with respect to IL-1β, TNF-α, and CCL5/RANTES showing the largest interindividual variations (Table 1). The effects of resveratrol (RV) and ω-3 PUFAs on the inflammatory mediators of activated PBLs are shown in Figure 1. In order to correct for interindividual variations, the data are expressed as a ratio [(substance + LPS-treated cells)/LPS-treated cells]. RV drastically reduced the secretion of PGE2, which is dependent on the LPS-induced expression of COX-2 in monocytes/macrophages (Table 1). Conversely, interleukin- (IL-) 1β, IL-6, and the anti-inflammatory IL-10 were increased in the presence of RV (6.25–25 μmol/L). We further investigated the impact of RV on chemokine secretion. CCL5/RANTES, which recruits activated T lymphocytes [24], was augmented by high concentrations of RV (25 μmol/L). The neutrophil recruiting chemokine CXCL8/IL-8 was blunted by increasing RV concentration. CCL2/MCP-1, which is involved in targeted migration of resident monocytes [24] and macrophage polarization [25], was not significantly altered. In contrast, RV enhanced production of CCL4/MIP-1β, a chemokine for subtypes of monocytes [26].


ω-3 PUFAs and Resveratrol Differently Modulate Acute and Chronic Inflammatory Processes.

Schwager J, Richard N, Riegger C, Salem N - Biomed Res Int (2015)

Production of inflammatory mediators by activated PBLs and its modulation by RV, DHA, and EPA. PBLs from healthy individuals were activated with LPS in the presence of the indicated substances (in μmol/L) and the inflammatory mediators in supernatants of 24 h cultures were determined in duplicate. The results are expressed as the ratio of substance + LPS-treated cells/LPS-treated cells. Data are given as mean ± SD of experiments obtained from PBL from at least four individuals. The dotted line (at ratio 1) indicates the no-effect level. ¤, ¥ indicate statistically significant differences observed in 50% and >75% of the donors, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537711&req=5

fig1: Production of inflammatory mediators by activated PBLs and its modulation by RV, DHA, and EPA. PBLs from healthy individuals were activated with LPS in the presence of the indicated substances (in μmol/L) and the inflammatory mediators in supernatants of 24 h cultures were determined in duplicate. The results are expressed as the ratio of substance + LPS-treated cells/LPS-treated cells. Data are given as mean ± SD of experiments obtained from PBL from at least four individuals. The dotted line (at ratio 1) indicates the no-effect level. ¤, ¥ indicate statistically significant differences observed in 50% and >75% of the donors, respectively.
Mentions: In order to investigate the effects of nutrients on acute inflammatory responses, PBLs from different individuals were activated with LPS in the presence of graded amounts of substances. LPS triggered substantial secretion of cytokines, chemokines, and PGE2; some of these differed considerably between individuals with respect to IL-1β, TNF-α, and CCL5/RANTES showing the largest interindividual variations (Table 1). The effects of resveratrol (RV) and ω-3 PUFAs on the inflammatory mediators of activated PBLs are shown in Figure 1. In order to correct for interindividual variations, the data are expressed as a ratio [(substance + LPS-treated cells)/LPS-treated cells]. RV drastically reduced the secretion of PGE2, which is dependent on the LPS-induced expression of COX-2 in monocytes/macrophages (Table 1). Conversely, interleukin- (IL-) 1β, IL-6, and the anti-inflammatory IL-10 were increased in the presence of RV (6.25–25 μmol/L). We further investigated the impact of RV on chemokine secretion. CCL5/RANTES, which recruits activated T lymphocytes [24], was augmented by high concentrations of RV (25 μmol/L). The neutrophil recruiting chemokine CXCL8/IL-8 was blunted by increasing RV concentration. CCL2/MCP-1, which is involved in targeted migration of resident monocytes [24] and macrophage polarization [25], was not significantly altered. In contrast, RV enhanced production of CCL4/MIP-1β, a chemokine for subtypes of monocytes [26].

Bottom Line: ω-3 PUFAs and polyphenols have multiple effects on inflammation in vivo and in vitro.Combination of RV and ω-3 PUFAs exerted synergistic effects on CCL5/RANTES and had additive effects on IL-6 or CXCL8/IL-8.Both ω-3 PUFAs and RV reduced catabolic gene expression (e.g., MMPs, ADAMTS-4, IL-1β, and IL-6) in activated chondrocytes.

View Article: PubMed Central - PubMed

Affiliation: DSM Nutritional Products Ltd., Department of Human Nutrition & Health, P.O. Box 2676, 4002 Basel, Switzerland.

ABSTRACT
ω-3 PUFAs and polyphenols have multiple effects on inflammation in vivo and in vitro. The effects of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and resveratrol (RV) were investigated in LPS-stimulated peripheral blood leukocytes (PBLs) (i.e., acute inflammation) and IL-1β activated human chondrocytes (i.e., chronic inflammation). Inflammatory mediators including chemokines, cytokines, interleukins, and PGE2 were measured by multiplex analysis and gene expression was quantified by RT-PCR. In PBLs, RV decreased the secretion of PGE2, CCL5/RANTES, and CXCL8/IL-8 but increased IL-1β, IL-6, and IL-10. In contrast to RV, ω-3 PUFAs augmented the production of PGE2 and CXCL8/IL-8. EPA and DHA similarly affected the pattern of inflammatory mediators. Combination of RV and ω-3 PUFAs exerted synergistic effects on CCL5/RANTES and had additive effects on IL-6 or CXCL8/IL-8. Both ω-3 PUFAs and RV reduced catabolic gene expression (e.g., MMPs, ADAMTS-4, IL-1β, and IL-6) in activated chondrocytes. The data suggest that ω-3 PUFAs and RV differ in the regulation of acute inflammation of peripheral blood leukocytes but have common properties in modulating features related to chronic inflammation of chondrocytes.

No MeSH data available.


Related in: MedlinePlus