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Comparative Effect of Lisinopril and Fosinopril in Mitigating Learning and Memory Deficit in Scopolamine-Induced Amnesic Rats.

Deb D, Bairy KL, Nayak V, Rao M - Adv Pharmacol Sci (2015)

Bottom Line: Scopolamine induced marked impairment of memory in behavioral tests which correlated with morphological changes in hippocampus.Pretreatment with fosinopril 1.80 mg/kg was found to significantly ameliorate the memory deficits and hippocampal degeneration induced by scopolamine.Fosinopril exhibits antiamnesic activity, indicating its possible role in preventing memory deficits seen in dementia though the precise mechanism underlying this effect needs to be further evaluated.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Melaka Manipal Medical College, Manipal University, Manipal Campus, Manipal 576104, India.

ABSTRACT
Lisinopril and fosinopril were compared on scopolamine-induced learning and memory deficits in rats. A total of eighty-four male Wistar rats were divided into seven groups. Group I received 2% gum acacia orally for 4 weeks, group II received normal saline, and group III received scopolamine (2 mg/kg/ip) as single dose. Groups IV and V received lisinopril ( 0.225 mg/kg and 0.45 mg/kg), while Groups VI and VII received fosinopril (0.90 mg/kg and 1.80 mg/kg), respectively, orally for four weeks, followed by scopolamine (2 mg/kg/ip) given 45 minutes prior to experimental procedure. Evaluation of learning and memory was assessed by using passive avoidance, Morris water maze, and elevated plus maze tests followed by analysis of hippocampal morphology and quantification of the number of surviving neurons. Scopolamine induced marked impairment of memory in behavioral tests which correlated with morphological changes in hippocampus. Pretreatment with fosinopril 1.80 mg/kg was found to significantly ameliorate the memory deficits and hippocampal degeneration induced by scopolamine. Fosinopril exhibits antiamnesic activity, indicating its possible role in preventing memory deficits seen in dementia though the precise mechanism underlying this effect needs to be further evaluated.

No MeSH data available.


Related in: MedlinePlus

Effect of lisinopril and fosinopril on the morphology of CA3 region of hippocampus in scopolamine-induced amnesic rats. Light photomicrographs of CA3 layer of hippocampus in (a) control rats, (b) scopolamine, (c) lisinopril (0.225 mg/kg) + scop., (d) lisinopril (0.45 mg/kg) + scop., (e) fosinopril (0.90 mg/kg) + scop., and (f) fosinopril (1.80 mg/kg) + scop. Scale bar represents 1 µ.
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fig3: Effect of lisinopril and fosinopril on the morphology of CA3 region of hippocampus in scopolamine-induced amnesic rats. Light photomicrographs of CA3 layer of hippocampus in (a) control rats, (b) scopolamine, (c) lisinopril (0.225 mg/kg) + scop., (d) lisinopril (0.45 mg/kg) + scop., (e) fosinopril (0.90 mg/kg) + scop., and (f) fosinopril (1.80 mg/kg) + scop. Scale bar represents 1 µ.

Mentions: In hematoxylin and eosin stained sections of hippocampal CA3 (Figure 3), CA1 (Figure 4), and dentate gyrus regions (Figure 5), cells with lightly stained nucleus, healthy cell membrane, and clear cytoplasm were considered as normal neurons while flame-shaped cells with pyknotic cell bodies, homogenous cytoplasm, and intense basophilic appearance were considered as damaged cells. Control rats and rats treated with higher dose of fosinopril demonstrated healthy neurons in all the three regions of the hippocampus compared to scopolamine treated rats which showed damaged neuronal cells. However, treatment with lower and higher doses of lisinopril could not markedly reverse the scopolamine-induced morphological changes produced in the hippocampus as demonstrated in Figures 3, 4, and 5.


Comparative Effect of Lisinopril and Fosinopril in Mitigating Learning and Memory Deficit in Scopolamine-Induced Amnesic Rats.

Deb D, Bairy KL, Nayak V, Rao M - Adv Pharmacol Sci (2015)

Effect of lisinopril and fosinopril on the morphology of CA3 region of hippocampus in scopolamine-induced amnesic rats. Light photomicrographs of CA3 layer of hippocampus in (a) control rats, (b) scopolamine, (c) lisinopril (0.225 mg/kg) + scop., (d) lisinopril (0.45 mg/kg) + scop., (e) fosinopril (0.90 mg/kg) + scop., and (f) fosinopril (1.80 mg/kg) + scop. Scale bar represents 1 µ.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537708&req=5

fig3: Effect of lisinopril and fosinopril on the morphology of CA3 region of hippocampus in scopolamine-induced amnesic rats. Light photomicrographs of CA3 layer of hippocampus in (a) control rats, (b) scopolamine, (c) lisinopril (0.225 mg/kg) + scop., (d) lisinopril (0.45 mg/kg) + scop., (e) fosinopril (0.90 mg/kg) + scop., and (f) fosinopril (1.80 mg/kg) + scop. Scale bar represents 1 µ.
Mentions: In hematoxylin and eosin stained sections of hippocampal CA3 (Figure 3), CA1 (Figure 4), and dentate gyrus regions (Figure 5), cells with lightly stained nucleus, healthy cell membrane, and clear cytoplasm were considered as normal neurons while flame-shaped cells with pyknotic cell bodies, homogenous cytoplasm, and intense basophilic appearance were considered as damaged cells. Control rats and rats treated with higher dose of fosinopril demonstrated healthy neurons in all the three regions of the hippocampus compared to scopolamine treated rats which showed damaged neuronal cells. However, treatment with lower and higher doses of lisinopril could not markedly reverse the scopolamine-induced morphological changes produced in the hippocampus as demonstrated in Figures 3, 4, and 5.

Bottom Line: Scopolamine induced marked impairment of memory in behavioral tests which correlated with morphological changes in hippocampus.Pretreatment with fosinopril 1.80 mg/kg was found to significantly ameliorate the memory deficits and hippocampal degeneration induced by scopolamine.Fosinopril exhibits antiamnesic activity, indicating its possible role in preventing memory deficits seen in dementia though the precise mechanism underlying this effect needs to be further evaluated.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Melaka Manipal Medical College, Manipal University, Manipal Campus, Manipal 576104, India.

ABSTRACT
Lisinopril and fosinopril were compared on scopolamine-induced learning and memory deficits in rats. A total of eighty-four male Wistar rats were divided into seven groups. Group I received 2% gum acacia orally for 4 weeks, group II received normal saline, and group III received scopolamine (2 mg/kg/ip) as single dose. Groups IV and V received lisinopril ( 0.225 mg/kg and 0.45 mg/kg), while Groups VI and VII received fosinopril (0.90 mg/kg and 1.80 mg/kg), respectively, orally for four weeks, followed by scopolamine (2 mg/kg/ip) given 45 minutes prior to experimental procedure. Evaluation of learning and memory was assessed by using passive avoidance, Morris water maze, and elevated plus maze tests followed by analysis of hippocampal morphology and quantification of the number of surviving neurons. Scopolamine induced marked impairment of memory in behavioral tests which correlated with morphological changes in hippocampus. Pretreatment with fosinopril 1.80 mg/kg was found to significantly ameliorate the memory deficits and hippocampal degeneration induced by scopolamine. Fosinopril exhibits antiamnesic activity, indicating its possible role in preventing memory deficits seen in dementia though the precise mechanism underlying this effect needs to be further evaluated.

No MeSH data available.


Related in: MedlinePlus