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Features of Blastocystis spp. in xenic culture revealed by deconvolutional microscopy.

Nagel R, Gray C, Bielefeldt-Ohmann H, Traub RJ - Parasitol. Res. (2015)

Bottom Line: Surface pores of 1 μm in diameter were observed cyclically opening and closing over 24 h.Vacuolated forms extruded a viscous material from a single surface point with coincident deflation that may demonstrate osmoregulation.Tear-shaped granules were observed exiting from the surface of an amoebic form, but their origin and identity remain unknown.

View Article: PubMed Central - PubMed

Affiliation: School of Veterinary Science, University of Queensland, Gatton, Queensland, Australia, robyn@tgclinic.com.au.

ABSTRACT
Blastocystis spp. are common human enteric parasites with complex morphology and have been reported to cause irritable bowel syndrome (IBS). Deconvolutional microscopy with time-lapse imaging and fluorescent spectroscopy of xenic cultures of Blastocystis spp. from stool samples of IBS patients and from asymptomatic, healthy pigs allowed observations of living organisms in their natural microbial environment. Blastocystis organisms of the vacuolated, granular, amoebic and cystic forms were observed to autofluorescence in the 557/576 emission spectra. Autofluorescence could be distinguished from fluorescein-conjugated Blastocystis-specific antibody labelling in vacuolated and granular forms. This antibody labelled Blastocystis subtypes 1, 3 and 4 but not 5. Surface pores of 1 μm in diameter were observed cyclically opening and closing over 24 h. Vacuolated forms extruded a viscous material from a single surface point with coincident deflation that may demonstrate osmoregulation. Tear-shaped granules were observed exiting from the surface of an amoebic form, but their origin and identity remain unknown.

No MeSH data available.


Related in: MedlinePlus

Blastocystis form in xenic culture showing pores on surface
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Fig8: Blastocystis form in xenic culture showing pores on surface

Mentions: Surface pores, with an approximate diameter of 1 μm, were seen on the external surface of the cell membrane of a partially ruptured ST3 Blastocystis organism (either a VF or a GF). The time-lapse recording of this event shows a total of 15 pores opening and closing (Supplementary video file 3). They appear to open and close fully over 1 h during four 15-min interval observations. The regular movement of the pores continued for the length of the 24 h study. A time interval sequence is shown with two pores in the foreground demonstrating fully open and closed pore positions in Fig. 8.Fig. 8


Features of Blastocystis spp. in xenic culture revealed by deconvolutional microscopy.

Nagel R, Gray C, Bielefeldt-Ohmann H, Traub RJ - Parasitol. Res. (2015)

Blastocystis form in xenic culture showing pores on surface
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537705&req=5

Fig8: Blastocystis form in xenic culture showing pores on surface
Mentions: Surface pores, with an approximate diameter of 1 μm, were seen on the external surface of the cell membrane of a partially ruptured ST3 Blastocystis organism (either a VF or a GF). The time-lapse recording of this event shows a total of 15 pores opening and closing (Supplementary video file 3). They appear to open and close fully over 1 h during four 15-min interval observations. The regular movement of the pores continued for the length of the 24 h study. A time interval sequence is shown with two pores in the foreground demonstrating fully open and closed pore positions in Fig. 8.Fig. 8

Bottom Line: Surface pores of 1 μm in diameter were observed cyclically opening and closing over 24 h.Vacuolated forms extruded a viscous material from a single surface point with coincident deflation that may demonstrate osmoregulation.Tear-shaped granules were observed exiting from the surface of an amoebic form, but their origin and identity remain unknown.

View Article: PubMed Central - PubMed

Affiliation: School of Veterinary Science, University of Queensland, Gatton, Queensland, Australia, robyn@tgclinic.com.au.

ABSTRACT
Blastocystis spp. are common human enteric parasites with complex morphology and have been reported to cause irritable bowel syndrome (IBS). Deconvolutional microscopy with time-lapse imaging and fluorescent spectroscopy of xenic cultures of Blastocystis spp. from stool samples of IBS patients and from asymptomatic, healthy pigs allowed observations of living organisms in their natural microbial environment. Blastocystis organisms of the vacuolated, granular, amoebic and cystic forms were observed to autofluorescence in the 557/576 emission spectra. Autofluorescence could be distinguished from fluorescein-conjugated Blastocystis-specific antibody labelling in vacuolated and granular forms. This antibody labelled Blastocystis subtypes 1, 3 and 4 but not 5. Surface pores of 1 μm in diameter were observed cyclically opening and closing over 24 h. Vacuolated forms extruded a viscous material from a single surface point with coincident deflation that may demonstrate osmoregulation. Tear-shaped granules were observed exiting from the surface of an amoebic form, but their origin and identity remain unknown.

No MeSH data available.


Related in: MedlinePlus