Limits...
Features of Blastocystis spp. in xenic culture revealed by deconvolutional microscopy.

Nagel R, Gray C, Bielefeldt-Ohmann H, Traub RJ - Parasitol. Res. (2015)

Bottom Line: Surface pores of 1 μm in diameter were observed cyclically opening and closing over 24 h.Vacuolated forms extruded a viscous material from a single surface point with coincident deflation that may demonstrate osmoregulation.Tear-shaped granules were observed exiting from the surface of an amoebic form, but their origin and identity remain unknown.

View Article: PubMed Central - PubMed

Affiliation: School of Veterinary Science, University of Queensland, Gatton, Queensland, Australia, robyn@tgclinic.com.au.

ABSTRACT
Blastocystis spp. are common human enteric parasites with complex morphology and have been reported to cause irritable bowel syndrome (IBS). Deconvolutional microscopy with time-lapse imaging and fluorescent spectroscopy of xenic cultures of Blastocystis spp. from stool samples of IBS patients and from asymptomatic, healthy pigs allowed observations of living organisms in their natural microbial environment. Blastocystis organisms of the vacuolated, granular, amoebic and cystic forms were observed to autofluorescence in the 557/576 emission spectra. Autofluorescence could be distinguished from fluorescein-conjugated Blastocystis-specific antibody labelling in vacuolated and granular forms. This antibody labelled Blastocystis subtypes 1, 3 and 4 but not 5. Surface pores of 1 μm in diameter were observed cyclically opening and closing over 24 h. Vacuolated forms extruded a viscous material from a single surface point with coincident deflation that may demonstrate osmoregulation. Tear-shaped granules were observed exiting from the surface of an amoebic form, but their origin and identity remain unknown.

No MeSH data available.


Related in: MedlinePlus

Fluorescent microscopy of Blastocystis forms stained with DAPI
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4537705&req=5

Fig10: Fluorescent microscopy of Blastocystis forms stained with DAPI

Mentions: Another time-lapse series of images showed extrusion of a viscous material apparently from one single point on the external cell membrane of two VFs of Blastocystis (Fig. 9) (Supplementary video file 4). The Blastocystis organisms were first seen to increase in size over 13 h then deflate, but without disintegrating, as this substance extruded. This extrusion process took 11.5 h in total, and the extruded material had an amoebic shape. A separate DAPI-stained slide (with multiple images taken at cross sections through the slide, but not time-lapse) stained nuclei in adjacent VFs bright blue, but there was no discrete nuclear material staining seen in the adjacent amoebic shape. This amoebic-shaped structure appeared very similar in shape to the extruded material observed in the previous slide (Fig. 10). The centre of this amoebic structure stained a light diffuse blue, and the surface is covered in granules that do not fluoresce with the DAPI stain.Fig. 9


Features of Blastocystis spp. in xenic culture revealed by deconvolutional microscopy.

Nagel R, Gray C, Bielefeldt-Ohmann H, Traub RJ - Parasitol. Res. (2015)

Fluorescent microscopy of Blastocystis forms stained with DAPI
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537705&req=5

Fig10: Fluorescent microscopy of Blastocystis forms stained with DAPI
Mentions: Another time-lapse series of images showed extrusion of a viscous material apparently from one single point on the external cell membrane of two VFs of Blastocystis (Fig. 9) (Supplementary video file 4). The Blastocystis organisms were first seen to increase in size over 13 h then deflate, but without disintegrating, as this substance extruded. This extrusion process took 11.5 h in total, and the extruded material had an amoebic shape. A separate DAPI-stained slide (with multiple images taken at cross sections through the slide, but not time-lapse) stained nuclei in adjacent VFs bright blue, but there was no discrete nuclear material staining seen in the adjacent amoebic shape. This amoebic-shaped structure appeared very similar in shape to the extruded material observed in the previous slide (Fig. 10). The centre of this amoebic structure stained a light diffuse blue, and the surface is covered in granules that do not fluoresce with the DAPI stain.Fig. 9

Bottom Line: Surface pores of 1 μm in diameter were observed cyclically opening and closing over 24 h.Vacuolated forms extruded a viscous material from a single surface point with coincident deflation that may demonstrate osmoregulation.Tear-shaped granules were observed exiting from the surface of an amoebic form, but their origin and identity remain unknown.

View Article: PubMed Central - PubMed

Affiliation: School of Veterinary Science, University of Queensland, Gatton, Queensland, Australia, robyn@tgclinic.com.au.

ABSTRACT
Blastocystis spp. are common human enteric parasites with complex morphology and have been reported to cause irritable bowel syndrome (IBS). Deconvolutional microscopy with time-lapse imaging and fluorescent spectroscopy of xenic cultures of Blastocystis spp. from stool samples of IBS patients and from asymptomatic, healthy pigs allowed observations of living organisms in their natural microbial environment. Blastocystis organisms of the vacuolated, granular, amoebic and cystic forms were observed to autofluorescence in the 557/576 emission spectra. Autofluorescence could be distinguished from fluorescein-conjugated Blastocystis-specific antibody labelling in vacuolated and granular forms. This antibody labelled Blastocystis subtypes 1, 3 and 4 but not 5. Surface pores of 1 μm in diameter were observed cyclically opening and closing over 24 h. Vacuolated forms extruded a viscous material from a single surface point with coincident deflation that may demonstrate osmoregulation. Tear-shaped granules were observed exiting from the surface of an amoebic form, but their origin and identity remain unknown.

No MeSH data available.


Related in: MedlinePlus