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Clindamycin Phosphate Absorption from Nanoliposomal Formulations through Third-Degree Burn Eschar.

Ghaffari A, Manafi A, Moghimi HR - World J Plast Surg (2015)

Bottom Line: Then the effect of liposomal lipid concentration on CP absorption was investigated.Data also showed that increasing liposomal lipid concentration from 20 to 100 mM, clindamycin permeation decreased by about 2 times.There was no difference between normal liposome and ultradeformable liposome in terms of clindamycin absorption.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran; ; Department of Food and Drug Control, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran;

ABSTRACT

Background: It has been shown that topical nanoliposomal formulations improve burn healing process. On the other hand, it has been shown that liposomal formulations increase drug deposition in the normal skin while decrease their systemic absorption; there is not such data available for burn eschar. Present investigation studies permeation of clindamycin phosphate (CP) through burn eschar from liposomal formulations to answer this question. In this investigation, permeation of CP through fully hydrated third-degree burn eschar was evaluated using solution, normal nanoliposomes and ultradeformable nanoliposomes.

Methods: Liposomal CP were prepared by thin-film hydration and characterized in terms of size, size distribution, zeta potential, encapsulation efficiency and short-time stability. Then the effect of liposomal lipid concentration on CP absorption was investigated.

Results: The permeability coefficient ratio (liposome/solution) and permeation lag-time ratio (liposome/solution) of CP through burn eschar at 20 Mm lipid concentration were 0.81±0.21 and 1.19±1.30 respectively, indicating the retardation effects of liposomes. Data also showed that increasing liposomal lipid concentration from 20 to 100 mM, clindamycin permeation decreased by about 2 times. There was no difference between normal liposome and ultradeformable liposome in terms of clindamycin absorption.

Conclusion: Nanoliposomes could decrease trans-eschar absorption of CP, in good agreement with normal skin data, and might indicate CP deposition in the eschar tissue.

No MeSH data available.


Related in: MedlinePlus

Permeatiom lag-time ratio (liposomal formulation/solution). Data are mean+SEM, n=3-7. Statistical comparisons in t-test for p<0.05 are shown as *(Non significant).
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Figure 2: Permeatiom lag-time ratio (liposomal formulation/solution). Data are mean+SEM, n=3-7. Statistical comparisons in t-test for p<0.05 are shown as *(Non significant).

Mentions: The permeability coefficient and permeation lag-time of CP permeated through burn eschars from CP solutions in different sets of experiments were calculated to be in the range of 4.23-1.48×10-3 cm/hr and 0.6-1.87 hr respectively. These ranges are broad because CP absorption tests were done on the samples that were obtained from different burned patients and also from different place of burning in one patient. So, differences between Kp and permeation lag-time of different eschars are due to biological variations of burn eschars. Therefore, in this study, to reduce the effect of such unavoidable variations, control (CP solution) and test (liposomal formulation) study of each set of experiment were performed on eschar samples prepared from a single eschar tissue. Even with such considerations, CV% of permeation parameters is relatively high but, still acceptable considering the complexity of burn eschar barrier (Table 3). Permeability coefficient (Kp) ratios, liposomal formulations to solutions Kps and also lag-time ratios, liposomal formulations / solutions lag time ratios, are shown in Table 3 and Figure 1 and 2.


Clindamycin Phosphate Absorption from Nanoliposomal Formulations through Third-Degree Burn Eschar.

Ghaffari A, Manafi A, Moghimi HR - World J Plast Surg (2015)

Permeatiom lag-time ratio (liposomal formulation/solution). Data are mean+SEM, n=3-7. Statistical comparisons in t-test for p<0.05 are shown as *(Non significant).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4537606&req=5

Figure 2: Permeatiom lag-time ratio (liposomal formulation/solution). Data are mean+SEM, n=3-7. Statistical comparisons in t-test for p<0.05 are shown as *(Non significant).
Mentions: The permeability coefficient and permeation lag-time of CP permeated through burn eschars from CP solutions in different sets of experiments were calculated to be in the range of 4.23-1.48×10-3 cm/hr and 0.6-1.87 hr respectively. These ranges are broad because CP absorption tests were done on the samples that were obtained from different burned patients and also from different place of burning in one patient. So, differences between Kp and permeation lag-time of different eschars are due to biological variations of burn eschars. Therefore, in this study, to reduce the effect of such unavoidable variations, control (CP solution) and test (liposomal formulation) study of each set of experiment were performed on eschar samples prepared from a single eschar tissue. Even with such considerations, CV% of permeation parameters is relatively high but, still acceptable considering the complexity of burn eschar barrier (Table 3). Permeability coefficient (Kp) ratios, liposomal formulations to solutions Kps and also lag-time ratios, liposomal formulations / solutions lag time ratios, are shown in Table 3 and Figure 1 and 2.

Bottom Line: Then the effect of liposomal lipid concentration on CP absorption was investigated.Data also showed that increasing liposomal lipid concentration from 20 to 100 mM, clindamycin permeation decreased by about 2 times.There was no difference between normal liposome and ultradeformable liposome in terms of clindamycin absorption.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran; ; Department of Food and Drug Control, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran;

ABSTRACT

Background: It has been shown that topical nanoliposomal formulations improve burn healing process. On the other hand, it has been shown that liposomal formulations increase drug deposition in the normal skin while decrease their systemic absorption; there is not such data available for burn eschar. Present investigation studies permeation of clindamycin phosphate (CP) through burn eschar from liposomal formulations to answer this question. In this investigation, permeation of CP through fully hydrated third-degree burn eschar was evaluated using solution, normal nanoliposomes and ultradeformable nanoliposomes.

Methods: Liposomal CP were prepared by thin-film hydration and characterized in terms of size, size distribution, zeta potential, encapsulation efficiency and short-time stability. Then the effect of liposomal lipid concentration on CP absorption was investigated.

Results: The permeability coefficient ratio (liposome/solution) and permeation lag-time ratio (liposome/solution) of CP through burn eschar at 20 Mm lipid concentration were 0.81±0.21 and 1.19±1.30 respectively, indicating the retardation effects of liposomes. Data also showed that increasing liposomal lipid concentration from 20 to 100 mM, clindamycin permeation decreased by about 2 times. There was no difference between normal liposome and ultradeformable liposome in terms of clindamycin absorption.

Conclusion: Nanoliposomes could decrease trans-eschar absorption of CP, in good agreement with normal skin data, and might indicate CP deposition in the eschar tissue.

No MeSH data available.


Related in: MedlinePlus