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The Healing Effect of Arnebia Euchroma Ointment versus Silver Sulfadiazine on Burn Wounds in Rat.

Nasiri E, Hosseinimehr SJ, Azadbakht M, Akbari J, Enayati-Fard R, Azizi S, Azadbakht M - World J Plast Surg (2015)

Bottom Line: Wound size was significantly lower in 10% AEO than 1% SSD and control groups on the 10(th) day post-burn injury.On day 11, the percentage of wound contraction in 5% and 10% AEO was 53.9%±14.7% and 55.9±10.5% which was more than 1% SSD (15.3±10.8%).The collagen fibers were well formed and horizontally-oriented in 5% and 10% AEO groups when compared with other groups.

View Article: PubMed Central - PubMed

Affiliation: Traditional and Complementary Medicine Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran;

ABSTRACT

Background: Burn is still a majordevastating condition in emergency medicine departments among both genders and all age groups in all developed and developing countries, leading to physical, psychological scars and economical burden. The present study aimed to determine the healing effect of topical treatment with Arnebia euchroma on second-degree burn wound in rats.

Methods: Fifty rats were divided into 4 equal groups receiving the ointment base, normal saline (NS), standard 1% silver sulfadiazine (SSD), and 5% and 10% Arnebia euchroma ointments (AEO). The mean of burn area, percentage of wound contraction, histopathological and bacteriological assessments in the injured area were dtermined during the study.

Results: Average area of wound on the 10(th) day was 10.2±2.3, 8.4±2.6, 12.4±2.5, 5.9±2.2 and 5.7±2 cm(2) for ointment base, NS, 1% SSD, and 5% and 10% AEO, respectively. Wound size was significantly lower in 10% AEO than 1% SSD and control groups on the 10(th) day post-burn injury. On day 11, the percentage of wound contraction in 5% and 10% AEO was 53.9%±14.7% and 55.9±10.5% which was more than 1% SSD (15.3±10.8%). The collagen fibers were well formed and horizontally-oriented in 5% and 10% AEO groups when compared with other groups.

Conclusion: Arnebia euchroma ointment was an effective treatment for healing of burn wounds in comparison with SSD and can be regarded as an alternative topical treatment for burn wounds.

No MeSH data available.


Related in: MedlinePlus

Comparison of the histopathology of samples from second degree burn wounds on 7th and 21st days after treatment with ointment base (BO), normal saline (N/S), silver sulfadiazine (1% SSD), and 5% Arnebia euchroma (5% A) and 10% Arnebia euchroma (10% A). Macrophage infiltration, neovascularization and fibroblastic proliferation were more in 10% A, and 5% A, compared with 1% SSD, BO and N/S groups on the 7th day. Also, thickness of granular cell layer and epidermal thickness extent were prominent in Arnebia euchroma groups in comparison with other groups on 7th and 21st days. A=5% Arnebia euchroma_7th day, A1=5% Arnebia euchroma_21st day, B=10% Arnebia euchroma_7th day, B1=10% Arnebia euchroma_21st day, C=1% Silver sulfadiazine_7th day, C1=1% Silver sulfadiazine_21st day, D=Ointment base_7th day, D1=Ointment base_21st day, E=Normal saline_7th day, E1=Normal saline_21st day.
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Figure 3: Comparison of the histopathology of samples from second degree burn wounds on 7th and 21st days after treatment with ointment base (BO), normal saline (N/S), silver sulfadiazine (1% SSD), and 5% Arnebia euchroma (5% A) and 10% Arnebia euchroma (10% A). Macrophage infiltration, neovascularization and fibroblastic proliferation were more in 10% A, and 5% A, compared with 1% SSD, BO and N/S groups on the 7th day. Also, thickness of granular cell layer and epidermal thickness extent were prominent in Arnebia euchroma groups in comparison with other groups on 7th and 21st days. A=5% Arnebia euchroma_7th day, A1=5% Arnebia euchroma_21st day, B=10% Arnebia euchroma_7th day, B1=10% Arnebia euchroma_21st day, C=1% Silver sulfadiazine_7th day, C1=1% Silver sulfadiazine_21st day, D=Ointment base_7th day, D1=Ointment base_21st day, E=Normal saline_7th day, E1=Normal saline_21st day.

Mentions: The extent of granulation tissue formation, matrix organization, re-epithelialization and formation of a new dermis were determined. On day 7, samples revealed an increase in macrophage and cellular infiltration in animals treated with 5 and 10% AEO in comparison to SSD and NS groups. The extent of maturation, tissue organization and re-epithelialization was more in groups treated with 5 and 19% AEO when compared with SSD and ointment base groups (Figure 3).


The Healing Effect of Arnebia Euchroma Ointment versus Silver Sulfadiazine on Burn Wounds in Rat.

Nasiri E, Hosseinimehr SJ, Azadbakht M, Akbari J, Enayati-Fard R, Azizi S, Azadbakht M - World J Plast Surg (2015)

Comparison of the histopathology of samples from second degree burn wounds on 7th and 21st days after treatment with ointment base (BO), normal saline (N/S), silver sulfadiazine (1% SSD), and 5% Arnebia euchroma (5% A) and 10% Arnebia euchroma (10% A). Macrophage infiltration, neovascularization and fibroblastic proliferation were more in 10% A, and 5% A, compared with 1% SSD, BO and N/S groups on the 7th day. Also, thickness of granular cell layer and epidermal thickness extent were prominent in Arnebia euchroma groups in comparison with other groups on 7th and 21st days. A=5% Arnebia euchroma_7th day, A1=5% Arnebia euchroma_21st day, B=10% Arnebia euchroma_7th day, B1=10% Arnebia euchroma_21st day, C=1% Silver sulfadiazine_7th day, C1=1% Silver sulfadiazine_21st day, D=Ointment base_7th day, D1=Ointment base_21st day, E=Normal saline_7th day, E1=Normal saline_21st day.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
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Figure 3: Comparison of the histopathology of samples from second degree burn wounds on 7th and 21st days after treatment with ointment base (BO), normal saline (N/S), silver sulfadiazine (1% SSD), and 5% Arnebia euchroma (5% A) and 10% Arnebia euchroma (10% A). Macrophage infiltration, neovascularization and fibroblastic proliferation were more in 10% A, and 5% A, compared with 1% SSD, BO and N/S groups on the 7th day. Also, thickness of granular cell layer and epidermal thickness extent were prominent in Arnebia euchroma groups in comparison with other groups on 7th and 21st days. A=5% Arnebia euchroma_7th day, A1=5% Arnebia euchroma_21st day, B=10% Arnebia euchroma_7th day, B1=10% Arnebia euchroma_21st day, C=1% Silver sulfadiazine_7th day, C1=1% Silver sulfadiazine_21st day, D=Ointment base_7th day, D1=Ointment base_21st day, E=Normal saline_7th day, E1=Normal saline_21st day.
Mentions: The extent of granulation tissue formation, matrix organization, re-epithelialization and formation of a new dermis were determined. On day 7, samples revealed an increase in macrophage and cellular infiltration in animals treated with 5 and 10% AEO in comparison to SSD and NS groups. The extent of maturation, tissue organization and re-epithelialization was more in groups treated with 5 and 19% AEO when compared with SSD and ointment base groups (Figure 3).

Bottom Line: Wound size was significantly lower in 10% AEO than 1% SSD and control groups on the 10(th) day post-burn injury.On day 11, the percentage of wound contraction in 5% and 10% AEO was 53.9%±14.7% and 55.9±10.5% which was more than 1% SSD (15.3±10.8%).The collagen fibers were well formed and horizontally-oriented in 5% and 10% AEO groups when compared with other groups.

View Article: PubMed Central - PubMed

Affiliation: Traditional and Complementary Medicine Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran;

ABSTRACT

Background: Burn is still a majordevastating condition in emergency medicine departments among both genders and all age groups in all developed and developing countries, leading to physical, psychological scars and economical burden. The present study aimed to determine the healing effect of topical treatment with Arnebia euchroma on second-degree burn wound in rats.

Methods: Fifty rats were divided into 4 equal groups receiving the ointment base, normal saline (NS), standard 1% silver sulfadiazine (SSD), and 5% and 10% Arnebia euchroma ointments (AEO). The mean of burn area, percentage of wound contraction, histopathological and bacteriological assessments in the injured area were dtermined during the study.

Results: Average area of wound on the 10(th) day was 10.2±2.3, 8.4±2.6, 12.4±2.5, 5.9±2.2 and 5.7±2 cm(2) for ointment base, NS, 1% SSD, and 5% and 10% AEO, respectively. Wound size was significantly lower in 10% AEO than 1% SSD and control groups on the 10(th) day post-burn injury. On day 11, the percentage of wound contraction in 5% and 10% AEO was 53.9%±14.7% and 55.9±10.5% which was more than 1% SSD (15.3±10.8%). The collagen fibers were well formed and horizontally-oriented in 5% and 10% AEO groups when compared with other groups.

Conclusion: Arnebia euchroma ointment was an effective treatment for healing of burn wounds in comparison with SSD and can be regarded as an alternative topical treatment for burn wounds.

No MeSH data available.


Related in: MedlinePlus