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Platelet-Rich Fibrin Promotes an Accelerated Healing of Achilles Tendon When Compared to Platelet-Rich Plasma in Rat.

Dietrich F, L Duré G, P Klein C, F Bampi V, V Padoin A, D Silva V, Braga-Silva J - World J Plast Surg (2015)

Bottom Line: On the basis of lead time, we compared the effect of using platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in repairing rat Achilles tendon.In Sirius red staining, no significant treatment differences were found between groups.In H&E staining, PRF showed a better cellular organization when compared to the other groups at 28 days.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Medical Abilities and Surgical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil;

ABSTRACT

Background: Autologous platelet concentrate has been used to improve the function and regeneration of injured tissues. Tendinopathies are common in clinical practice, although long-term treatment is required. On the basis of lead time, we compared the effect of using platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in repairing rat Achilles tendon.

Methods: The effectiveness of using PRP and PRF was evaluated after 14 and 28 postoperative days by histological analysis. The quantification of collagen types I and III was performed by Sirius red staining. Qualitatively, the data were verified with hematoxylin-eosin (H&E) staining.

Results: In Sirius red staining, no significant treatment differences were found between groups. Statistical difference was observed only between PRP (37.2% collagen) and the control group (16.2%) 14 days after treatment. Intra-groups compared twice showed a difference for collagen I (27.8% and 47.7%) and III (66.9% and 46.0%) in the PRF group. The control group showed differences only in collagen I (14.2% and 40.9%) and no other finding was observed in the PRP group. In H&E staining, PRF showed a better cellular organization when compared to the other groups at 28 days.

Conclusion: Our study suggests that PRF promotes accelerated regeneration of the Achilles tendon in rats, offering promising prospects for future clinical use.

No MeSH data available.


Related in: MedlinePlus

Images captured with Sirius red staining (SRS) in a longitudinal section after treatments, at 14 (A, C, E) and 28 (B, D, F) days. Magnification: X50 Bar: 100 µm
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Figure 4: Images captured with Sirius red staining (SRS) in a longitudinal section after treatments, at 14 (A, C, E) and 28 (B, D, F) days. Magnification: X50 Bar: 100 µm

Mentions: Comparing type I collagen areas at the two times (14 and 28 days), a statistical difference was noticed in the control (p=0.01) and PRF (p<0.05) groups. The PRP group remained stable for the collagen type I and III indices over time. However, only the PRF group showed a statistical difference both to type I as to type III collagen indices between the two evaluated time periods (p<0.05) (Figure 3), and this can also be checked in the SRS images (Figure 4). Ckecking this information, there was a significant type I collagen increase and a type III collagen decrease in PRF group over time, suggesting a better healing process.


Platelet-Rich Fibrin Promotes an Accelerated Healing of Achilles Tendon When Compared to Platelet-Rich Plasma in Rat.

Dietrich F, L Duré G, P Klein C, F Bampi V, V Padoin A, D Silva V, Braga-Silva J - World J Plast Surg (2015)

Images captured with Sirius red staining (SRS) in a longitudinal section after treatments, at 14 (A, C, E) and 28 (B, D, F) days. Magnification: X50 Bar: 100 µm
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4537601&req=5

Figure 4: Images captured with Sirius red staining (SRS) in a longitudinal section after treatments, at 14 (A, C, E) and 28 (B, D, F) days. Magnification: X50 Bar: 100 µm
Mentions: Comparing type I collagen areas at the two times (14 and 28 days), a statistical difference was noticed in the control (p=0.01) and PRF (p<0.05) groups. The PRP group remained stable for the collagen type I and III indices over time. However, only the PRF group showed a statistical difference both to type I as to type III collagen indices between the two evaluated time periods (p<0.05) (Figure 3), and this can also be checked in the SRS images (Figure 4). Ckecking this information, there was a significant type I collagen increase and a type III collagen decrease in PRF group over time, suggesting a better healing process.

Bottom Line: On the basis of lead time, we compared the effect of using platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in repairing rat Achilles tendon.In Sirius red staining, no significant treatment differences were found between groups.In H&E staining, PRF showed a better cellular organization when compared to the other groups at 28 days.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Medical Abilities and Surgical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil;

ABSTRACT

Background: Autologous platelet concentrate has been used to improve the function and regeneration of injured tissues. Tendinopathies are common in clinical practice, although long-term treatment is required. On the basis of lead time, we compared the effect of using platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in repairing rat Achilles tendon.

Methods: The effectiveness of using PRP and PRF was evaluated after 14 and 28 postoperative days by histological analysis. The quantification of collagen types I and III was performed by Sirius red staining. Qualitatively, the data were verified with hematoxylin-eosin (H&E) staining.

Results: In Sirius red staining, no significant treatment differences were found between groups. Statistical difference was observed only between PRP (37.2% collagen) and the control group (16.2%) 14 days after treatment. Intra-groups compared twice showed a difference for collagen I (27.8% and 47.7%) and III (66.9% and 46.0%) in the PRF group. The control group showed differences only in collagen I (14.2% and 40.9%) and no other finding was observed in the PRP group. In H&E staining, PRF showed a better cellular organization when compared to the other groups at 28 days.

Conclusion: Our study suggests that PRF promotes accelerated regeneration of the Achilles tendon in rats, offering promising prospects for future clinical use.

No MeSH data available.


Related in: MedlinePlus