Limits...
YKL-40 regulated epithelial-mesenchymal transition and migration/invasion enhancement in non-small cell lung cancer.

Jefri M, Huang YN, Huang WC, Tai CS, Chen WL - BMC Cancer (2015)

Bottom Line: YKL-40 is a secreted inflammatory protein that its overexpression has been reported to correlate with poor outcome of various malignant diseases, especially in cancer.Furthermore, determined by the PrognoScan database analysis, patients with high expression levels of YKL-40 were found with poor prognosis.In this study,YKL-40 was demonstrated to regulate EMT marker expressions such as Twist, Snail, Slug, N-cadherin, Vimentin, and E-cadherin.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 1001 University Road, Hsinchu, Taiwan, 300, ROC. malvinjefri@hotmail.com.

ABSTRACT

Background: YKL-40 is a secreted inflammatory protein that its overexpression has been reported to correlate with poor outcome of various malignant diseases, especially in cancer. However, the function of this protein is still unclear.

Methods: The clinical prognosis of non-small cell lung cancers (NSCLC) patients and their clinical YKL-40 expressions were obtained from the Prognoscan database. The expressions of YKL-40 in patient samples were determined by Western Blotting assay. YKL-40 gene knockdown and overexpression were performed on NSCLC cancer cells (CL1-1 and CL1-5). The cells were investigated for their epithelial-mesenchymal transition (EMT) markers gene modulation through Western Blotting and RT-PCR. Further cell metastatic abilities were assessed by transwell migration and invasion assay.

Result: In this study, YKL-40 was observed to be highly expressed in NSCLC specimens. Furthermore, determined by the PrognoScan database analysis, patients with high expression levels of YKL-40 were found with poor prognosis. In the in vitro study, different characteristics of NSCLC cell lines (CL1-1, H23, H838, CL1-5, and H2009) were used as study models, where YKL-40 expression levels were determined to correlate with the phenotypic characteristics of cancer metastasis. In this study,YKL-40 was demonstrated to regulate EMT marker expressions such as Twist, Snail, Slug, N-cadherin, Vimentin, and E-cadherin. The protein's affects in cancer cell migration and invasion were also observed in YKL-40 overexpression or knock down NSCLC cell lines.

Conclusion: All of results from this study suggest that YKL-40 is a major factor in NSCLC metastasis. Thus, YKL-40 may serve as therapeutic targets for NSCLC patients in the future.

No MeSH data available.


Related in: MedlinePlus

The effect of YKL-40 expression level on the invasion and migration abilities of the NSCLC cell lines. a The five NSCLC cell lines were divided into low metastasis (CL1-1, H23) and high metastasis (H838, CL1-5, H2009) groups by invasion and migration assay. b The YKL-40 expression level analysis by reverse transcription polymerase chain reaction (RT-PCR) and Western blot: indicating that the low and high metastasis groups expressed reduced and increased levels of YKL-40 mRNA and protein, respectively
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4537570&req=5

Fig2: The effect of YKL-40 expression level on the invasion and migration abilities of the NSCLC cell lines. a The five NSCLC cell lines were divided into low metastasis (CL1-1, H23) and high metastasis (H838, CL1-5, H2009) groups by invasion and migration assay. b The YKL-40 expression level analysis by reverse transcription polymerase chain reaction (RT-PCR) and Western blot: indicating that the low and high metastasis groups expressed reduced and increased levels of YKL-40 mRNA and protein, respectively

Mentions: To investigate the effect of YKL-40 expression level for tumor migration and invasion in NSCLC, five NSCLC cell lines (CL 1–1, CL 1–5, H23, H838, and H2009) were used for the evaluation of their migration/invasion abilities and YKL-40 expression levels. As shown in Fig. 2a, cell lines that had high migration activities were correlated, in general, with their high invasion. The cells were divided into low (CL 1–1 and H23) and high (H838, CL 1–5 and H2009) migration/invasion groups. Figure 2b shows that the cell lines with higher migration/invasion (H838, CL 1–5 and H2009) had greater YKL-40 mRNA and protein expression levels (determined by RT-PCR and Western blot), when compared with those that exhibited lower migration/invasion abilities (CL 1–1 and H23).Fig. 2


YKL-40 regulated epithelial-mesenchymal transition and migration/invasion enhancement in non-small cell lung cancer.

Jefri M, Huang YN, Huang WC, Tai CS, Chen WL - BMC Cancer (2015)

The effect of YKL-40 expression level on the invasion and migration abilities of the NSCLC cell lines. a The five NSCLC cell lines were divided into low metastasis (CL1-1, H23) and high metastasis (H838, CL1-5, H2009) groups by invasion and migration assay. b The YKL-40 expression level analysis by reverse transcription polymerase chain reaction (RT-PCR) and Western blot: indicating that the low and high metastasis groups expressed reduced and increased levels of YKL-40 mRNA and protein, respectively
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4537570&req=5

Fig2: The effect of YKL-40 expression level on the invasion and migration abilities of the NSCLC cell lines. a The five NSCLC cell lines were divided into low metastasis (CL1-1, H23) and high metastasis (H838, CL1-5, H2009) groups by invasion and migration assay. b The YKL-40 expression level analysis by reverse transcription polymerase chain reaction (RT-PCR) and Western blot: indicating that the low and high metastasis groups expressed reduced and increased levels of YKL-40 mRNA and protein, respectively
Mentions: To investigate the effect of YKL-40 expression level for tumor migration and invasion in NSCLC, five NSCLC cell lines (CL 1–1, CL 1–5, H23, H838, and H2009) were used for the evaluation of their migration/invasion abilities and YKL-40 expression levels. As shown in Fig. 2a, cell lines that had high migration activities were correlated, in general, with their high invasion. The cells were divided into low (CL 1–1 and H23) and high (H838, CL 1–5 and H2009) migration/invasion groups. Figure 2b shows that the cell lines with higher migration/invasion (H838, CL 1–5 and H2009) had greater YKL-40 mRNA and protein expression levels (determined by RT-PCR and Western blot), when compared with those that exhibited lower migration/invasion abilities (CL 1–1 and H23).Fig. 2

Bottom Line: YKL-40 is a secreted inflammatory protein that its overexpression has been reported to correlate with poor outcome of various malignant diseases, especially in cancer.Furthermore, determined by the PrognoScan database analysis, patients with high expression levels of YKL-40 were found with poor prognosis.In this study,YKL-40 was demonstrated to regulate EMT marker expressions such as Twist, Snail, Slug, N-cadherin, Vimentin, and E-cadherin.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 1001 University Road, Hsinchu, Taiwan, 300, ROC. malvinjefri@hotmail.com.

ABSTRACT

Background: YKL-40 is a secreted inflammatory protein that its overexpression has been reported to correlate with poor outcome of various malignant diseases, especially in cancer. However, the function of this protein is still unclear.

Methods: The clinical prognosis of non-small cell lung cancers (NSCLC) patients and their clinical YKL-40 expressions were obtained from the Prognoscan database. The expressions of YKL-40 in patient samples were determined by Western Blotting assay. YKL-40 gene knockdown and overexpression were performed on NSCLC cancer cells (CL1-1 and CL1-5). The cells were investigated for their epithelial-mesenchymal transition (EMT) markers gene modulation through Western Blotting and RT-PCR. Further cell metastatic abilities were assessed by transwell migration and invasion assay.

Result: In this study, YKL-40 was observed to be highly expressed in NSCLC specimens. Furthermore, determined by the PrognoScan database analysis, patients with high expression levels of YKL-40 were found with poor prognosis. In the in vitro study, different characteristics of NSCLC cell lines (CL1-1, H23, H838, CL1-5, and H2009) were used as study models, where YKL-40 expression levels were determined to correlate with the phenotypic characteristics of cancer metastasis. In this study,YKL-40 was demonstrated to regulate EMT marker expressions such as Twist, Snail, Slug, N-cadherin, Vimentin, and E-cadherin. The protein's affects in cancer cell migration and invasion were also observed in YKL-40 overexpression or knock down NSCLC cell lines.

Conclusion: All of results from this study suggest that YKL-40 is a major factor in NSCLC metastasis. Thus, YKL-40 may serve as therapeutic targets for NSCLC patients in the future.

No MeSH data available.


Related in: MedlinePlus