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Correlation of cumulus gene expression of GJA1, PRSS35, PTX3, and SERPINE2 with oocyte maturation, fertilization, and embryo development.

Li SH, Lin MH, Hwu YM, Lu CH, Yeh LY, Chen YJ, Lee RK - Reprod. Biol. Endocrinol. (2015)

Bottom Line: PRSS35 mRNA levels in cumulus cells of fertilized oocytes were significantly higher than those in cumulus cells of unfertilized oocytes.GJA1 and SERPINE2 seemed to express higher mRNA levels, while PRSS35 showed lower expression in cumulus cells of oocytes that developed into embryos with good morphology; however, the expression levels of all three genes and PTX3 showed no significant differences between embryos with good or poor morphology.GJA1 and SERPINE2 represent potential gene markers associated with oocyte maturation.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Research, Mackay Memorial Hospital, Tamsui District, New Taipei City, 251, Taiwan. lsh@mmh.org.tw.

ABSTRACT

Background: GJA1 and PTX3 were proposed as gene markers for oocyte and embryo developmental competence, while SERPINE2 was reported to be associated with pregnancy outcome. PRSS35, which is exclusively expressed in the ovary, may be correlated with oocyte competence. This study was conducted to evaluate the correlation of cumulus GJA1, PRSS35, PTX3, and SERPINE2 gene expression levels with oocyte maturation, fertilization, and early embryo development.

Methods: In total, 308 cumulus cell samples separated from individual cumulus-oocyte complex were obtained from 40 patients undergoing the intracytoplasmic sperm injection treatment procedure. Gene expression levels (mRNA levels) in cumulus cells were assessed using quantitative real-time polymerase chain reaction.

Results: Gene expression levels of GJA1 and SERPINE2 in cumulus cells surrounding mature oocytes were significantly lower than those in cumulus cells enclosing immature oocytes. PRSS35 mRNA levels in cumulus cells of fertilized oocytes were significantly higher than those in cumulus cells of unfertilized oocytes. GJA1 and SERPINE2 seemed to express higher mRNA levels, while PRSS35 showed lower expression in cumulus cells of oocytes that developed into embryos with good morphology; however, the expression levels of all three genes and PTX3 showed no significant differences between embryos with good or poor morphology.

Conclusions: GJA1 and SERPINE2 represent potential gene markers associated with oocyte maturation. PRSS35 may be correlated with oocyte fertilization potential. However, GJA1, PRSS35, PTX3, and SERPINE2 may not be considered as marker genes for predicting embryo morphology.

No MeSH data available.


qRT-PCR revealed relative mRNA levels of GJA1 (a), PRSS35 (b), PTX3 (c), and SERPINE2 (d) in cumulus cells surrounding immature or mature oocytes. **P < 0.01; ***P < 0.001
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Fig2: qRT-PCR revealed relative mRNA levels of GJA1 (a), PRSS35 (b), PTX3 (c), and SERPINE2 (d) in cumulus cells surrounding immature or mature oocytes. **P < 0.01; ***P < 0.001

Mentions: Cumulus cells surrounding mature oocytes expressed significantly lower mRNA levels of GJA1 and SERPINE2 than those from immature oocytes (P = 0.0034 and P = 0.0002, respectively). On the other hand, PRSS35 and PTX3 mRNA levels did not show significant differences (Fig. 2).Fig. 2


Correlation of cumulus gene expression of GJA1, PRSS35, PTX3, and SERPINE2 with oocyte maturation, fertilization, and embryo development.

Li SH, Lin MH, Hwu YM, Lu CH, Yeh LY, Chen YJ, Lee RK - Reprod. Biol. Endocrinol. (2015)

qRT-PCR revealed relative mRNA levels of GJA1 (a), PRSS35 (b), PTX3 (c), and SERPINE2 (d) in cumulus cells surrounding immature or mature oocytes. **P < 0.01; ***P < 0.001
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4537566&req=5

Fig2: qRT-PCR revealed relative mRNA levels of GJA1 (a), PRSS35 (b), PTX3 (c), and SERPINE2 (d) in cumulus cells surrounding immature or mature oocytes. **P < 0.01; ***P < 0.001
Mentions: Cumulus cells surrounding mature oocytes expressed significantly lower mRNA levels of GJA1 and SERPINE2 than those from immature oocytes (P = 0.0034 and P = 0.0002, respectively). On the other hand, PRSS35 and PTX3 mRNA levels did not show significant differences (Fig. 2).Fig. 2

Bottom Line: PRSS35 mRNA levels in cumulus cells of fertilized oocytes were significantly higher than those in cumulus cells of unfertilized oocytes.GJA1 and SERPINE2 seemed to express higher mRNA levels, while PRSS35 showed lower expression in cumulus cells of oocytes that developed into embryos with good morphology; however, the expression levels of all three genes and PTX3 showed no significant differences between embryos with good or poor morphology.GJA1 and SERPINE2 represent potential gene markers associated with oocyte maturation.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Research, Mackay Memorial Hospital, Tamsui District, New Taipei City, 251, Taiwan. lsh@mmh.org.tw.

ABSTRACT

Background: GJA1 and PTX3 were proposed as gene markers for oocyte and embryo developmental competence, while SERPINE2 was reported to be associated with pregnancy outcome. PRSS35, which is exclusively expressed in the ovary, may be correlated with oocyte competence. This study was conducted to evaluate the correlation of cumulus GJA1, PRSS35, PTX3, and SERPINE2 gene expression levels with oocyte maturation, fertilization, and early embryo development.

Methods: In total, 308 cumulus cell samples separated from individual cumulus-oocyte complex were obtained from 40 patients undergoing the intracytoplasmic sperm injection treatment procedure. Gene expression levels (mRNA levels) in cumulus cells were assessed using quantitative real-time polymerase chain reaction.

Results: Gene expression levels of GJA1 and SERPINE2 in cumulus cells surrounding mature oocytes were significantly lower than those in cumulus cells enclosing immature oocytes. PRSS35 mRNA levels in cumulus cells of fertilized oocytes were significantly higher than those in cumulus cells of unfertilized oocytes. GJA1 and SERPINE2 seemed to express higher mRNA levels, while PRSS35 showed lower expression in cumulus cells of oocytes that developed into embryos with good morphology; however, the expression levels of all three genes and PTX3 showed no significant differences between embryos with good or poor morphology.

Conclusions: GJA1 and SERPINE2 represent potential gene markers associated with oocyte maturation. PRSS35 may be correlated with oocyte fertilization potential. However, GJA1, PRSS35, PTX3, and SERPINE2 may not be considered as marker genes for predicting embryo morphology.

No MeSH data available.