Limits...
Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae).

Goulart TM, de Castro CF, Machado VE, da Rocha Silva FB, Pinto MC - Parasit Vectors (2015)

Bottom Line: The most critical phase in sand fly colonization is the high mortality in the larval instars.The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae.Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Zoologia Animal, Universidade Estadual de Campinas, Campinas, SP, 13083-970, Brazil. thamarchi@gmail.com.

ABSTRACT

Background: The most critical phase in sand fly colonization is the high mortality in the larval instars. In this study, we sought out strategies for improving the colonization of Nyssomyia neivai, one of the vectors of cutaneous leishmaniasis agent in South America.

Methods: A colony of Ny. neivai was established in the laboratory from a field population, and the productivity of adults was evaluated considering carrying capacity, diet for larvae and surface for oviposition.

Results: The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae.

Conclusion: Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.

No MeSH data available.


Related in: MedlinePlus

Emergency of adults on non-sterilized and sterilized diets. Means with same letters are not significantly different (P>0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4537559&req=5

Fig4: Emergency of adults on non-sterilized and sterilized diets. Means with same letters are not significantly different (P>0.05)

Mentions: There were no significant differences among the treatments (p > 0.05). However, the means were higher with both of the sterilized methods, autoclave (46.7 insects) and tyndallization (47.8 insects), compared to only 38.4 insects in the non-autoclaved diet (Fig. 4).Fig. 4


Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae).

Goulart TM, de Castro CF, Machado VE, da Rocha Silva FB, Pinto MC - Parasit Vectors (2015)

Emergency of adults on non-sterilized and sterilized diets. Means with same letters are not significantly different (P>0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4537559&req=5

Fig4: Emergency of adults on non-sterilized and sterilized diets. Means with same letters are not significantly different (P>0.05)
Mentions: There were no significant differences among the treatments (p > 0.05). However, the means were higher with both of the sterilized methods, autoclave (46.7 insects) and tyndallization (47.8 insects), compared to only 38.4 insects in the non-autoclaved diet (Fig. 4).Fig. 4

Bottom Line: The most critical phase in sand fly colonization is the high mortality in the larval instars.The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae.Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Zoologia Animal, Universidade Estadual de Campinas, Campinas, SP, 13083-970, Brazil. thamarchi@gmail.com.

ABSTRACT

Background: The most critical phase in sand fly colonization is the high mortality in the larval instars. In this study, we sought out strategies for improving the colonization of Nyssomyia neivai, one of the vectors of cutaneous leishmaniasis agent in South America.

Methods: A colony of Ny. neivai was established in the laboratory from a field population, and the productivity of adults was evaluated considering carrying capacity, diet for larvae and surface for oviposition.

Results: The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae.

Conclusion: Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.

No MeSH data available.


Related in: MedlinePlus