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Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae).

Goulart TM, de Castro CF, Machado VE, da Rocha Silva FB, Pinto MC - Parasit Vectors (2015)

Bottom Line: The most critical phase in sand fly colonization is the high mortality in the larval instars.The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae.Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Zoologia Animal, Universidade Estadual de Campinas, Campinas, SP, 13083-970, Brazil. thamarchi@gmail.com.

ABSTRACT

Background: The most critical phase in sand fly colonization is the high mortality in the larval instars. In this study, we sought out strategies for improving the colonization of Nyssomyia neivai, one of the vectors of cutaneous leishmaniasis agent in South America.

Methods: A colony of Ny. neivai was established in the laboratory from a field population, and the productivity of adults was evaluated considering carrying capacity, diet for larvae and surface for oviposition.

Results: The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae.

Conclusion: Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.

No MeSH data available.


Related in: MedlinePlus

Number of larvae on four different diets over time. Diet 1 (rabbit feces, rabbit food, fish food, and soil); 2 (rabbit feces and soil); 3 (rabbit feces, soil and fish food); and 4 (soil and mud). P < 0.05
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Fig2: Number of larvae on four different diets over time. Diet 1 (rabbit feces, rabbit food, fish food, and soil); 2 (rabbit feces and soil); 3 (rabbit feces, soil and fish food); and 4 (soil and mud). P < 0.05

Mentions: According to the results, the most attractive larval food was diet 1, followed by diet 3 (Fig. 2). Both diets contained fish food. Differences were significant among the four diets (p < 0.05), but there were no significant differences between diets 1 and 3 (p > 0.05) or 2 and 4 (p > 0.05). The presence of fish food appears to be an important factor in the attractiveness of a diet to the larval instars of Ny. neivai.Fig. 2


Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae).

Goulart TM, de Castro CF, Machado VE, da Rocha Silva FB, Pinto MC - Parasit Vectors (2015)

Number of larvae on four different diets over time. Diet 1 (rabbit feces, rabbit food, fish food, and soil); 2 (rabbit feces and soil); 3 (rabbit feces, soil and fish food); and 4 (soil and mud). P < 0.05
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4537559&req=5

Fig2: Number of larvae on four different diets over time. Diet 1 (rabbit feces, rabbit food, fish food, and soil); 2 (rabbit feces and soil); 3 (rabbit feces, soil and fish food); and 4 (soil and mud). P < 0.05
Mentions: According to the results, the most attractive larval food was diet 1, followed by diet 3 (Fig. 2). Both diets contained fish food. Differences were significant among the four diets (p < 0.05), but there were no significant differences between diets 1 and 3 (p > 0.05) or 2 and 4 (p > 0.05). The presence of fish food appears to be an important factor in the attractiveness of a diet to the larval instars of Ny. neivai.Fig. 2

Bottom Line: The most critical phase in sand fly colonization is the high mortality in the larval instars.The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae.Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Zoologia Animal, Universidade Estadual de Campinas, Campinas, SP, 13083-970, Brazil. thamarchi@gmail.com.

ABSTRACT

Background: The most critical phase in sand fly colonization is the high mortality in the larval instars. In this study, we sought out strategies for improving the colonization of Nyssomyia neivai, one of the vectors of cutaneous leishmaniasis agent in South America.

Methods: A colony of Ny. neivai was established in the laboratory from a field population, and the productivity of adults was evaluated considering carrying capacity, diet for larvae and surface for oviposition.

Results: The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae.

Conclusion: Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.

No MeSH data available.


Related in: MedlinePlus