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Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae).

Goulart TM, de Castro CF, Machado VE, da Rocha Silva FB, Pinto MC - Parasit Vectors (2015)

Bottom Line: The most critical phase in sand fly colonization is the high mortality in the larval instars.The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae.Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Zoologia Animal, Universidade Estadual de Campinas, Campinas, SP, 13083-970, Brazil. thamarchi@gmail.com.

ABSTRACT

Background: The most critical phase in sand fly colonization is the high mortality in the larval instars. In this study, we sought out strategies for improving the colonization of Nyssomyia neivai, one of the vectors of cutaneous leishmaniasis agent in South America.

Methods: A colony of Ny. neivai was established in the laboratory from a field population, and the productivity of adults was evaluated considering carrying capacity, diet for larvae and surface for oviposition.

Results: The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae.

Conclusion: Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.

No MeSH data available.


Related in: MedlinePlus

Percentages of adult emerged per number of eggs in different size containers. a ● five couples, ▄ ten couples, ▲fifteen couples, ○ twenty couples, ♦ twenty five couples, * thirty couples in 250 mL and b ● five couples, ▄ ten couples, ▲fifteen couples in 150 mL containers
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Fig1: Percentages of adult emerged per number of eggs in different size containers. a ● five couples, ▄ ten couples, ▲fifteen couples, ○ twenty couples, ♦ twenty five couples, * thirty couples in 250 mL and b ● five couples, ▄ ten couples, ▲fifteen couples in 150 mL containers

Mentions: The number of adults emerged was negatively correlated with the number of eggs per rearing container. Adult emergency rates were over 50 % in the 250 mL containers that contained approximately 400 eggs. For 150 mL containers, the emergency rates were 30 % (around 600 eggs per pot). The best results for both the smaller and larger containers were seen with five to ten insect couples (Fig. 1).Fig. 1


Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae).

Goulart TM, de Castro CF, Machado VE, da Rocha Silva FB, Pinto MC - Parasit Vectors (2015)

Percentages of adult emerged per number of eggs in different size containers. a ● five couples, ▄ ten couples, ▲fifteen couples, ○ twenty couples, ♦ twenty five couples, * thirty couples in 250 mL and b ● five couples, ▄ ten couples, ▲fifteen couples in 150 mL containers
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4537559&req=5

Fig1: Percentages of adult emerged per number of eggs in different size containers. a ● five couples, ▄ ten couples, ▲fifteen couples, ○ twenty couples, ♦ twenty five couples, * thirty couples in 250 mL and b ● five couples, ▄ ten couples, ▲fifteen couples in 150 mL containers
Mentions: The number of adults emerged was negatively correlated with the number of eggs per rearing container. Adult emergency rates were over 50 % in the 250 mL containers that contained approximately 400 eggs. For 150 mL containers, the emergency rates were 30 % (around 600 eggs per pot). The best results for both the smaller and larger containers were seen with five to ten insect couples (Fig. 1).Fig. 1

Bottom Line: The most critical phase in sand fly colonization is the high mortality in the larval instars.The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae.Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Zoologia Animal, Universidade Estadual de Campinas, Campinas, SP, 13083-970, Brazil. thamarchi@gmail.com.

ABSTRACT

Background: The most critical phase in sand fly colonization is the high mortality in the larval instars. In this study, we sought out strategies for improving the colonization of Nyssomyia neivai, one of the vectors of cutaneous leishmaniasis agent in South America.

Methods: A colony of Ny. neivai was established in the laboratory from a field population, and the productivity of adults was evaluated considering carrying capacity, diet for larvae and surface for oviposition.

Results: The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae.

Conclusion: Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.

No MeSH data available.


Related in: MedlinePlus